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2.4.1.20: cellobiose phosphorylase

This is an abbreviated version!
For detailed information about cellobiose phosphorylase, go to the full flat file.

Word Map on EC 2.4.1.20

Reaction

cellobiose
+
phosphate
=
alpha-D-glucose 1-phosphate
 +
D-glucose

Synonyms

Cbp, CbpA, cellobiose phosphorylase A, cellobiose:orthophosphate alpha-D-glucosyltransferase, CPase, CuCbP, GH94 cellobiose phosphorylase, lactose phosphorylase, ra2122, THA_1941, Tm_1848

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.20 cellobiose phosphorylase

Substrates Products

Substrates Products on EC 2.4.1.20 - cellobiose phosphorylase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,5-anhydroglucitol + glucose-1-phosphate
?
show the reaction diagram
-
-
-
-
?
alpha-D -glucose 1-fluoride + D-glucose
cellobiose + fluoride
show the reaction diagram
-
-
-
-
ir
alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
? + phosphate
show the reaction diagram
alpha-D-glucose 1-phosphate + 2-deoxy-2-fluoro-D-glucose
?
show the reaction diagram
-
-
-
-
?
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
?
show the reaction diagram
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
? + phosphate
show the reaction diagram
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
phosphate + ?
show the reaction diagram
-
-
-
r
alpha-D-glucose 1-phosphate + 5a-carba-alpha-D,L-glucopyranose
4-O-beta-D-glucopyranosyl-5a-carba-beta-D-glucopyranose + phosphate
show the reaction diagram
-
very poor substrate
-
-
?
alpha-D-glucose 1-phosphate + 5a-carba-beta-D,L-glucopyranose
?
show the reaction diagram
-
much better substrate than alpha-anomer, 22% of activity with D-glucose
-
-
?
alpha-D-glucose 1-phosphate + 6-deoxy-6-fluoro-D-glucose
?
show the reaction diagram
-
-
-
-
?
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
?
show the reaction diagram
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
? + phosphate
show the reaction diagram
alpha-D-glucose 1-phosphate + butyl beta-D-glucoside
? + phosphate
show the reaction diagram
-
no substrate for wild-type
-
-
r
alpha-D-glucose 1-phosphate + cellobiose
? + phosphate
show the reaction diagram
-
no substrate for wilalpha-D-type
-
-
r
alpha-D-glucose 1-phosphate + D-altrose
?
show the reaction diagram
alpha-D-glucose 1-phosphate + D-arabinose
?
show the reaction diagram
alpha-D-glucose 1-phosphate + D-arabinose
phosphate + ?
show the reaction diagram
-
-
-
r
alpha-D-glucose 1-phosphate + D-fructose
phosphate + ?
show the reaction diagram
-
-
-
r
alpha-D-glucose 1-phosphate + D-galactose
phosphate + ?
show the reaction diagram
-
-
-
r
alpha-D-glucose 1-phosphate + D-gentobiose
? + phosphate
show the reaction diagram
-
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucosamine
4-O-beta-D-glucopyranosyl-D-glucosamine
show the reaction diagram
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-glucosamine
?
show the reaction diagram
alpha-D-glucose 1-phosphate + D-glucosamine
? + phosphate
show the reaction diagram
-
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucosamine
phosphate + ?
show the reaction diagram
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucose
? + phosphate
show the reaction diagram
-
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucose
cellobiose + phosphate
show the reaction diagram
alpha-D-glucose 1-phosphate + D-maltose
phosphate + ?
show the reaction diagram
-
-
-
r
alpha-D-glucose 1-phosphate + D-mannose
4-O-beta-D-glucopyranosyl-D-mannose
show the reaction diagram
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-mannose
?
show the reaction diagram
alpha-D-glucose 1-phosphate + D-mannose
? + phosphate
show the reaction diagram
-
-
-
-
r
alpha-D-glucose 1-phosphate + D-mannose
phosphate + ?
show the reaction diagram
-
-
-
r
alpha-D-glucose 1-phosphate + D-xylose
?
show the reaction diagram
alpha-D-glucose 1-phosphate + D-xylose
? + phosphate
show the reaction diagram
-
-
-
-
r
alpha-D-glucose 1-phosphate + D-xylose
phosphate + ?
show the reaction diagram
-
-
-
r
alpha-D-glucose 1-phosphate + ethyl beta-D-glucoside
? + phosphate
show the reaction diagram
-
no substrate for wild-type
-
-
r
alpha-D-glucose 1-phosphate + L-fucose
?
show the reaction diagram
alpha-D-glucose 1-phosphate + L-galactose
?
show the reaction diagram
alpha-D-glucose 1-phosphate + methyl alpha-D-glucoside
? + phosphate
show the reaction diagram
-
no substrate for wild-type
-
-
r
alpha-D-glucose 1-phosphate + methyl beta-D-glucoside
? + phosphate
show the reaction diagram
-
no substrate for wild-type
-
-
r
alpha-D-glucose 1-phosphate + N-acetyl-D-glucosamine
4-O-beta-D-glucopyranosyl-N-acetyl-D-glucosamine
show the reaction diagram
-
substrate for mutant Y648V only
-
-
?
alpha-D-glucose 1-phosphate + N-acetyl-D-glucosamine
phosphate + ?
show the reaction diagram
-
-
-
r
alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside
? + phosphate
show the reaction diagram
-
no substrate for wild-type
-
-
r
Avicel + phosphate
? + D-glucose
show the reaction diagram
beta-D-glucose 1-phosphate + alpha-D-glucopyranosyl fluoride
beta-cellobiose + HF
show the reaction diagram
-
beta-D-glucopyranosyl fluoride is not ulilized
-
?
cellobiose + arsenate
D-glucose + alpha-D-glucose 1-arsenate
show the reaction diagram
-
-
alpha-D-glucose 1-arsenate hydrolyses spontaneously to form D-glucose and arsenate
ir
cellobiose + phosphate
alpha-D-glucose 1 phosphate + D-glucose
show the reaction diagram
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
show the reaction diagram
cellobiose + phosphate
D-glucose 1-phosphate + D-glucose
show the reaction diagram
-
-
-
-
?
cellopentaose + phosphate
?
show the reaction diagram
63.3% activity compared to cellotetraose
-
-
r
cellotetraose + phosphate
?
show the reaction diagram
100% activity
-
-
r
cellotriose + phosphate
?
show the reaction diagram
89.7% activity compared to cellotetraose
-
-
r
D-glucal + 2-deoxy-2-(hydroxymethyl)-beta-D-xylopyranose
2-deoxy-3-O-(2-deoxy-beta-D-arabino-hexopyranosyl)-2-(hydroxymethyl)-beta-D-xylopyranose + ?
show the reaction diagram
-
-
-
-
r
D-glucal + D-glucose
2-deoxy-beta-D-arabino-hexopyranosyl-(1->4)-D-glucose + ?
show the reaction diagram
-
-
-
-
r
D-glucal + D-mannose
2-deoxy-beta-D-arabino-hexopyranosyl-(1->4)-D-mannose + ?
show the reaction diagram
-
-
-
-
r
D-glucal + D-xylose
2-deoxy-beta-D-arabino-hexopyranosyl-(1->4)-D-xylose + ?
show the reaction diagram
-
-
-
-
r
D-glucal + phosphate
2-deoxy-alpha-D-glucose 1-phosphate
show the reaction diagram
-
D-glucal is a slow alternative donor substrate for stereospecific glycosyl transfer to phosphate. Reaction follows a stereochemical course where substrate becomes protonated from below its six-membered ring through stereoselective re side attack at C-2. The proposed catalytic mechanism involves direct protonation of D-glucal by the enzyme-bound phosphate, which then performs nucleophilic attack on the reactive C-1 of donor substrate. When offered D-glucose next to D-glucal and phosphate, the enzyme produces 2-deoxy-beta-D-glucosyl-(1->4)-D-glucose and 2-deoxy-alpha-D-glucose 1-phosphate in a ratio governed by mass action of the two acceptor substrates present. Enzymatic synthesis of 2-deoxy-beta-D-glucosyl-(1->4)-D-glucose is effectively promoted by catalytic concentrations of phosphate, suggesting that catalytic reaction proceeds through a quaternary complex of enzyme, D-glucal, phosphate,and D-glucose
-
-
?
gentiobiose + alpha-D-glucose 1-phosphate
?
show the reaction diagram
-
-
-
-
?
gentiobiose + phosphate
?
show the reaction diagram
-
-
-
r
isomaltose + alpha-D-glucose 1-phosphate
?
show the reaction diagram
-
-
-
-
?
lactose + phosphate
alpha-D-galactose 1-phosphate + D-glucose
show the reaction diagram
-
-
-
r
melibiose + alpha-D-glucose 1-phosphate
?
show the reaction diagram
-
-
-
-
?
phosphoric acid swollen cellulose + phosphate
? + D-glucose
show the reaction diagram
sugarcane bagasse + phosphate
? + D-glucose
show the reaction diagram
additional information
?
-