2.4.1.20: cellobiose phosphorylase

This is an abbreviated version!
For detailed information about cellobiose phosphorylase, go to the full flat file.

Reaction

Synonyms

Cbp, CbpA, cellobiose phosphorylase A, cellobiose:orthophosphate alpha-D-glucosyltransferase, CPase, CuCbP, GH94 cellobiose phosphorylase, lactose phosphorylase, ra2122, THA_1941, Tm_1848

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.20 cellobiose phosphorylase

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Results	in table
1	Activating Compound
146	AA Sequence
8	Application
1	CAS Registry Number
32	Cloned(Commentary)
3	Crystallization (Commentary)
2	Expression
2	General Information
3	General Stability
30	Inhibitors
58	kcat/KM [mM/s]
10	Ki Value [mM]
124	KM Value [mM]
7	Metals/Ions
19	Molecular Weight [Da]
49	Natural Substrates/ Products (Substrates)
6	Organic Solvent Stability
112	Organism
4	Pathway
28	PDB ID
19	pH Optimum
6	pH Range
3	pH Stability
26	Protein Variants
21	Purification (Commentary)
4	Reaction
1	Reaction Type
65	Reference
32	Specific Activity [micromol/min/mg]
205	Substrates and Products (Substrate)
18	Subunits
35	Synonyms
1	Systematic Name
14	Temperature Optimum [°C]
4	Temperature Range [°C]
15	Temperature Stability [°C]
91	Turnover Number [1/s]

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

2.8

1,5-anhydro-D-glucitol

Ruminococcus albus

-

pH 6.0, 37°C

719128

3.5

1,5-anhydroglucitol

Cellulomonas gilvus

-

enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0)

671874

8

2-deoxy-2-fluoro-D-glucose

Cellulomonas uda

-

-

637865

7.83 - 119

2-deoxy-D-glucose

7 entries

44.6

6-deoxy-6-fluoro-D-glucose

Cellulomonas uda

-

-

637865

17 - 124

6-deoxy-D-glucose

4 entries

1.3 - 96.5

alpha-D-glucose 1-phosphate

7 entries

9

butyl beta-D-glucoside

Cellulomonas uda

-

mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C

719136

0.23 - 88.9

cellobiose

20 entries

0.34

cellopentaose

Thermosipho africanus

B7IED6

at pH 7.5 and 75°C

758413

0.49

cellotetraose

Thermosipho africanus

B7IED6

at pH 7.5 and 75°C

758413

0.28

cellotriose

Thermosipho africanus

B7IED6

at pH 7.5 and 75°C

758413

4

D-gentobiose

Ruminococcus albus

-

pH 6.0, 37°C

719128

5.2 - 35.4

D-glucosamine

5 entries

5.3 - 92.3

D-glucose

9 entries

5.6

D-glucoseamine

Cellulomonas uda

-

-

637865

0.626 - 58.3

D-mannose

8 entries

1.37 - 44.3

D-xylose

5 entries

5.1 - 8.7

ethyl beta-D-glucoside

2 entries

7.2

gentiobiose

Cellulomonas gilvus

-

enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0)

671874

1.9

isomaltose

Cellulomonas gilvus

-

enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0)

671874

0.109 - 1.11

lactose

4 entries

2.3

melibiose

Cellulomonas gilvus

-

enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0)

671874

6

methyl alpha-D-glucoside

Cellulomonas uda

-

mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C

719136

6.8 - 7.9

methyl beta-D-glucoside

2 entries

6.54

N-acetyl-D-glucosamine

Ruminococcus albus

-

mutant enzyme Y648V, at pH 6.0 and 37°C

735893

4

phenyl beta-D-glucoside

Cellulomonas uda

-

mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C

719136

15.4

phosphate

2 entries

7.83

2-deoxy-D-glucose

Ruminococcus albus

-

mutant enzyme Y648V, at pH 6.0 and 37°C

735893

11.6

2-deoxy-D-glucose

Cellulomonas uda

-

-

637865

16

2-deoxy-D-glucose

Thermotoga maritima

-

-

637868

18.3

2-deoxy-D-glucose

Acetivibrio thermocellus

-

-

637869

21.5

2-deoxy-D-glucose

Ruminococcus albus

-

pH 6.0, 37°C

719128

21.5

2-deoxy-D-glucose

Ruminococcus albus

-

wild type enzyme, at pH 6.0 and 37°C

735893

119

2-deoxy-D-glucose

Ruminococcus albus

-

mutant enzyme Y648F, at pH 6.0 and 37°C

735893

17

6-deoxy-D-glucose

Thermotoga maritima

-

-

637868

55.3

6-deoxy-D-glucose

Cellulomonas uda

-

-

637865

59.5

6-deoxy-D-glucose

Acetivibrio thermocellus

-

-

637869

124

6-deoxy-D-glucose

Ruminococcus albus

-

pH 6.0, 37°C

719128

1.3

alpha-D-glucose 1-phosphate

Ruminococcus albus

-

mutant enzyme Y648V, at pH 6.0 and 37°C

735893

7.61

alpha-D-glucose 1-phosphate

Thermosipho africanus

B7IED6

at pH 7.5 and 75°C

758413

43.2

alpha-D-glucose 1-phosphate

Cellulomonas uda

-

pH 6.6, 30°C, native enzyme

659742

43.2

alpha-D-glucose 1-phosphate

Cellulomonas uda

-

pH 6.6, 30°C, recombinant His6-tagged enzyme

659742

64.2

alpha-D-glucose 1-phosphate

Ruminococcus albus

-

mutant enzyme Y648F, at pH 6.0 and 37°C

735893

92.3

alpha-D-glucose 1-phosphate

Ruminococcus albus

-

wild type enzyme, at pH 6.0 and 37°C

735893

96.5

alpha-D-glucose 1-phosphate

Ruminococcus albus

-

mutant enzyme C485A, at pH 6.0 and 37°C

735893

0.23

cellobiose

Thermosipho africanus

B7IED6

at pH 7.5 and 75°C

758413

1.1

cellobiose

Thermoclostridium stercorarium

Q59316

-

489244

1.3

cellobiose

Acetivibrio thermocellus

-

mutant S411G, pH 7.2, 40°C

720434

1.5

cellobiose

Acetivibrio thermocellus

-

mutant R48R/Q130H/K131Y/K142R/S411G/A423S/V526A/A781K, pH 7.2, 40°C

720434

2.01

cellobiose

Cellulomonas uda

Q7WTR6

pH 6.6, 37°C, recombinant mutant T508I/N667A

706588

2.1

cellobiose

Acetivibrio thermocellus

-

mutant Q130H/K131Y/S411G/A423S/A781K, pH 7.2, 40°C

720434

2.6

cellobiose

Cellulomonas uda

-

mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C

719136

2.61

cellobiose

Cellulomonas uda

Q7WTR6

pH 6.6, 37°C, recombinant mutant T508A/N667T

706588

2.9

cellobiose

Acetivibrio thermocellus

-

mutant A423S, pH 7.2, 40°C

720434

2.9

cellobiose

Acetivibrio thermocellus

-

mutant A781 K, pH 7.2, 40°C

720434

3.28

cellobiose

Cellulomonas uda

Q7WTR6

pH 6.6, 37°C, recombinant mutant A397V/T508A/A512T/D557N/N667T/G681S

706588

3.4

cellobiose

Acetivibrio thermocellus

-

wild-type, pH 7.2, 40°C

720434

5.4

cellobiose

Thermotoga maritima

-

-

637868

13.2

cellobiose

Cellulomonas uda

Q7WTR6

pH 6.6, 37°C, recombinant wild-type enzyme

706588

29.1

cellobiose

Cellulomonas uda

-

-

637865

29.1

cellobiose

Cellulomonas uda

-

pH 6.6, 30°C, native enzyme

659742

29.1

cellobiose

Cellulomonas uda

-

pH 6.6, 30°C, recombinant His6-tagged enzyme

659742

73.9

cellobiose

Ruminococcus albus

-

mutant enzyme Y648F, at pH 6.0 and 37°C

735893

79.1

cellobiose

Ruminococcus albus

-

wild type enzyme, at pH 6.0 and 37°C

735893

88.9

cellobiose

Ruminococcus albus

-

mutant enzyme C485A, at pH 6.0 and 37°C

735893

5.2

D-glucosamine

Thermotoga maritima

-

-

637868

7.7

D-glucosamine

Acetivibrio thermocellus

-

-

637869

9.56

D-glucosamine

Ruminococcus albus

-

pH 6.0, 37°C

719128

9.56

D-glucosamine

Ruminococcus albus

-

wild type enzyme, at pH 6.0 and 37°C

735893

35.4

D-glucosamine

Ruminococcus albus

-

mutant enzyme C485A, at pH 6.0 and 37°C

735893

5.3

D-glucose

Cellulomonas uda

-

mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C

719136

6.3

D-glucose

Cellulomonas uda

-

mutant T508I/E649C/N667A, pH 6.6, 37°C

719136

8

D-glucose

Thermotoga maritima

-

-

637868

12.2

D-glucose

Acetivibrio thermocellus

-

-

637869

27.5

D-glucose

Cellulomonas uda

-

pH 6.6, 30°C, native enzyme

659742

27.5

D-glucose

Cellulomonas uda

-

pH 6.6, 30°C, recombinant His6-tagged enzyme

659742

30.3

D-glucose

Thermosipho africanus

B7IED6

at pH 7.5 and 75°C

758413

43.2

D-glucose

Cellulomonas uda

-

-

637865

92.3

D-glucose

Ruminococcus albus

-

pH 6.0, 37°C

719128

0.626

D-mannose

Ruminococcus albus

-

mutant enzyme C485A, at pH 6.0 and 37°C

735893

2.83

D-mannose

Ruminococcus albus

-

pH 6.0, 37°C

719128

2.83

D-mannose

Ruminococcus albus

-

wild type enzyme, at pH 6.0 and 37°C

735893

4.4

D-mannose

Thermotoga maritima

-

-

637868

5.27

D-mannose

Ruminococcus albus

-

mutant enzyme Y648V, at pH 6.0 and 37°C

735893

6.6

D-mannose

Cellulomonas uda

-

-

637865

9.3

D-mannose

Acetivibrio thermocellus

-

-

637869

58.3

D-mannose

Ruminococcus albus

-

mutant enzyme Y648F, at pH 6.0 and 37°C

735893

1.37

D-xylose

Thermosipho africanus

B7IED6

at pH 7.5 and 75°C

758413

8.7

D-xylose

Cellulomonas uda

-

-

637865

16.5

D-xylose

Ruminococcus albus

-

pH 6.0, 37°C

719128

40

D-xylose

Thermotoga maritima

-

-

637868

44.3

D-xylose

Acetivibrio thermocellus

-

-

637869

5.1

ethyl beta-D-glucoside

Cellulomonas uda

-

mutant T508I/E649C/N667A, pH 6.6, 37°C

719136

8.7

ethyl beta-D-glucoside

Cellulomonas uda

-

mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C

719136

0.109

lactose

Cellulomonas uda

Q7WTR6

pH 6.6, 37°C, recombinant wild-type enzyme

706588

0.35

lactose

Cellulomonas uda

Q7WTR6

pH 6.6, 37°C, recombinant mutant T508I/N667A

706588

0.53

lactose

Cellulomonas uda

Q7WTR6

pH 6.6, 37°C, recombinant mutant T508A/N667T

706588

1.11

lactose

Cellulomonas uda

Q7WTR6

pH 6.6, 37°C, recombinant mutant A397V/T508A/A512T/D557N/N667T/G681S

706588

6.8

methyl beta-D-glucoside

Cellulomonas uda

-

mutant T508I/E649C/N667A, pH 6.6, 37°C

719136

7.9

methyl beta-D-glucoside

Cellulomonas uda

-

mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C

719136

15.4

phosphate

Cellulomonas uda

-

pH 6.6, 30°C, native enzyme

659742

15.4

phosphate

Cellulomonas uda

-

pH 6.6, 30°C, recombinant His6-tagged enzyme

659742