Any feedback?
Literature summary for 2.4.1.20 extracted from
- Purification and properties of a cellobiose phosphorylase (CepA) and a cellodextrin phosphorylase (CepB) from the cellulolytic thermophile Clostridium stercorarium (1997), Eur. J. Biochem., 247, 262-267.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Thermoclostridium stercorarium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.2 | - |
cellobiose | - |
Thermoclostridium stercorarium |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 93000 | - |
SDS-PAGE, gel filtration | Thermoclostridium stercorarium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + phosphate | Thermoclostridium stercorarium | - |
alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
| cellobiose + phosphate | Thermoclostridium stercorarium NCIB 11754 | - |
alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoclostridium stercorarium | Q59316 | - |
- |
| Thermoclostridium stercorarium NCIB 11754 | Q59316 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoclostridium stercorarium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.7 | - |
purified enzyme | Thermoclostridium stercorarium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + phosphate | - |
Thermoclostridium stercorarium | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
| cellobiose + phosphate | - |
Thermoclostridium stercorarium NCIB 11754 | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
| additional information | only disaccharide accepted as substrate | Thermoclostridium stercorarium | ? | - |
? | |
| additional information | only disaccharide accepted as substrate | Thermoclostridium stercorarium NCIB 11754 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 93000, SDS-PAGE, gel filtration | Thermoclostridium stercorarium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Thermoclostridium stercorarium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable for at least 42 h | Thermoclostridium stercorarium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
cellobiose | - |
Thermoclostridium stercorarium | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Thermoclostridium stercorarium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | 50% activity | Thermoclostridium stercorarium |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
