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Literature summary for 2.4.1.20 extracted from
- Simple protein purification through affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage (2008), J. Chromatogr. A, 1194, 150-154.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of intein-tagged enzyme in Escherichia coli strain BL21, subcloning in Escherichia coli strain DH5alpha | Acetivibrio thermocellus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme with a cellobiose-binding intein tag, development of a simple, low-cost, and scalable protein purification method using a biodegradable regenerated amorphous cellulose, affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage, method evaluation, overview | Acetivibrio thermocellus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Acetivibrio thermocellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Acetivibrio thermocellus |
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Release 2023.1 (January 2023)
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