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0.77 - 13
2-oxo-3-methylbutanoate
10.7
2-oxo-butanoate
-
pH 7.4, 25°C
7
2-oxo-n-pentanoate
-
pH 7.4, 25°C
0.49 - 1.08
2-oxoisovalerate
105
3-Fluoropyruvate
pH 7.5, 25°C
1.2 - 450
3-methyl-2-oxobutanoate
0.28
3-methyl-2-oxopentanoate
pH 7.5, 25°C
0.12
4-methylthio-2-oxobutyrate
isoform IPMS3, pH and temperature not specified in the publication
1.36
alpha-Ketobutyrate
pH 7.5, 25°C
0.022 - 4.75
alpha-ketoisovalerate
0.52 - 1.17
alpha-ketoisovaleric acid
0.04
propionyl-CoA
-
hydrolysis, absence of MgCl2, pH 7.4, 25°C
0.77
2-oxo-3-methylbutanoate
-
pH 7.4, 25°C, presence of Co2+
1.69
2-oxo-3-methylbutanoate
-
pH 7.4, 25°C, presence of Mn2+
2.3
2-oxo-3-methylbutanoate
-
2.3
2-oxo-3-methylbutanoate
pH 8.0, isoform IPMS2
2.4
2-oxo-3-methylbutanoate
-
2.4
2-oxo-3-methylbutanoate
pH 8.0, isoform IPMS1
2.9
2-oxo-3-methylbutanoate
-
pH 7.4, 25°C, presence of Rb+
3.2
2-oxo-3-methylbutanoate
-
pH 7.4, 25°C, presence of K+
3.5
2-oxo-3-methylbutanoate
-
pH 7.4, 25°C
3.5
2-oxo-3-methylbutanoate
-
pH 7.4, 25°C, presence of Mg2+
3.5
2-oxo-3-methylbutanoate
full-length enzyme, in 50 mM HEPES (pH 7.5), at 25°C
13
2-oxo-3-methylbutanoate
full-length enzyme, in 50 mM HEPES (pH 7.5), at 25°C
0.49
2-oxoisovalerate
isoform IPMS3, pH and temperature not specified in the publication
1.08
2-oxoisovalerate
isoform IPMS1, pH and temperature not specified in the publication
1.2
3-methyl-2-oxobutanoate
pH 8.0, 50°C
3.1
3-methyl-2-oxobutanoate
mutant enzyme D444A, at pH 7.5 and 25°C
3.4
3-methyl-2-oxobutanoate
mutant enzyme D444Y, at pH 7.5 and 25°C
3.7
3-methyl-2-oxobutanoate
wild type enzyme, at pH 7.5 and 25°C
5.7
3-methyl-2-oxobutanoate
mutant enzyme D444R, at pH 7.5 and 25°C
7
3-methyl-2-oxobutanoate
mutant enzyme R97A, at pH 7.5 and 25°C
7.28
3-methyl-2-oxobutanoate
-
isoform Leu9, at pH 7.5 and 30°C
13.79
3-methyl-2-oxobutanoate
-
isoform Leu4, at pH 7.5 and 30°C
330
3-methyl-2-oxobutanoate
pH 7.8, 37°C
330
3-methyl-2-oxobutanoate
-
at pH 7.8 and 37°C
450
3-methyl-2-oxobutanoate
pH 7.8, 55°C
0.03
acetyl-CoA
-
hydrolysis, presence of MgCl2, pH 7.4, 25°C
0.05
acetyl-CoA
-
mutant enzyme D81A, at pH 7.5 and 25°C
0.058
acetyl-CoA
mutant E218A, enzyme displays substrate activation, unactivated form
0.06
acetyl-CoA
-
hydrolysis, absence of MgCl2, pH 7.4, 25°C
0.093
acetyl-CoA
mutant E218A, enzyme displays substrate activation, activated form
0.093
acetyl-CoA
mutant Y410F
0.12
acetyl-CoA
-
mutant enzyme D81E, at pH 7.5 and 25°C
0.13
acetyl-CoA
-
mutant enzyme D81E/N321I, at pH 7.5 and 25°C
0.15
acetyl-CoA
-
mutant enzyme N321A, at pH 7.5 and 25°C
0.15
acetyl-CoA
-
mutant enzyme N321I, at pH 7.5 and 25°C
0.32
acetyl-CoA
-
wild type enzyme, at pH 7.5 and 25°C
0.61
acetyl-CoA
alpha-IPMS-14CR
1.7
acetyl-CoA
pH 8.0, 50°C
1.9
acetyl-CoA
pH 8.0, isoform IPMS2
2.1
acetyl-CoA
-
cosubstrate 2-oxo-3-methylbutanoate, pH 7.4, 25°C
2.2
acetyl-CoA
pH 8.0, isoform IPMS1
2.22
acetyl-CoA
alpha-IPMS-2CR
2.55
acetyl-CoA
mutant H379A
2.717
acetyl-CoA
wild-type
3.5
acetyl-CoA
full-length enzyme, in 50 mM HEPES (pH 7.5), at 25°C
4.5
acetyl-CoA
-
cosubstrate pyruvate, pH 7.4, 25°C
5
acetyl-CoA
-
cosubstrate 2-oxo-n-pentanoate, pH 7.4, 25°C
5.667
acetyl-CoA
mutant Y169F
7.6
acetyl-CoA
-
cosubstrate 2-oxo-butanoate, pH 7.4, 25°C
13
acetyl-CoA
full-length enzyme, in 50 mM HEPES (pH 7.5), at 25°C
330
acetyl-CoA
pH 7.8, 37°C
450
acetyl-CoA
pH 7.8, 55°C
0.022
alpha-ketoisovalerate
mutant E218A, enzyme displays substrate activation, activated form
0.035
alpha-ketoisovalerate
pH 7.5, 25°C
0.037
alpha-ketoisovalerate
mutant E218A, enzyme displays substrate activation, unactivated form
0.075
alpha-ketoisovalerate
mutant Y410F
0.7
alpha-ketoisovalerate
mutant H379A, enzyme displays substrate activation, unactivated form
1.483
alpha-ketoisovalerate
mutant H379A, enzyme displays substrate activation, activated form
2.53
alpha-ketoisovalerate
wild-type
4.75
alpha-ketoisovalerate
mutant Y169F
0.52
alpha-ketoisovaleric acid
alpha-IPMS-14CR
1.17
alpha-ketoisovaleric acid
alpha-IPMS-2CR
0.04
crotonyl-CoA
-
hydrolysis, presence of MgCl2, pH 7.4, 25°C
0.05
crotonyl-CoA
-
hydrolysis, absence of MgCl2, pH 7.4, 25°C
1.5
pyruvate
pH 7.5, 25°C
6.1
pyruvate
-
pH 7.4, 25°C