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2.3.3.13: 2-isopropylmalate synthase

This is an abbreviated version!
For detailed information about 2-isopropylmalate synthase, go to the full flat file.

Word Map on EC 2.3.3.13

Reaction

acetyl-CoA
+
3-methyl-2-oxobutanoate
+
H2O
=
(2S)-2-isopropylmalate
+
CoA

Synonyms

2-isopropylmalate synthase 1, chloroplastic, 2-isopropylmalate synthase 2, chloroplastic, 3-hydroxy-4-methyl-3-carboxy-pentanoate 2-oxo-3-methyl-butanoate lyase (CoA-acetylating), 3-hydroxy-4-methyl-3-carboxyvalerate 2-oxo-3-methyl-butyrate lyase (CoA-acetylating), alpha-IPM synthase, Alpha-IPM synthetase, alpha-IPMS, alpha-IPMS-14CR, alpha-IPMS-2CR, Alpha-isopropylmalate synthase, alpha-isopropylmalate synthase I, alpha-isopropylmalate synthase II, alpha-isopropylmalate synthetase, alpha-isopropylmalic synthetase, BatIMS, EC 4.1.3.12, IPM synthase, IPM-synthase, IPMS, IPMS1, IPMS2, IPMS3, isopropylmalate synthase, isopropylmalate synthase 1, isopropylmalate synthetase, LEU4, LEU9, leuA, Mj1195, MtalphaIPMS, MtIPMS, NmeIPMS, Os11g04670, Os12g04440, OsIPMS1, OsIPMS2, ROL17, synthase, 2-isopropylmalate, synthase, alpha-isopropylmalate, TT_C0849

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.3 Acyl groups converted into alkyl groups on transfer
                2.3.3.13 2-isopropylmalate synthase

Molecular Weight

Molecular Weight on EC 2.3.3.13 - 2-isopropylmalate synthase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
-
in presence of Leu, gel filtration
114500
-
in absence of Leu, gel filtration
121000
-
in absence of Leu, gel filtration
124000
127000
-
sedimentation equilibrium centrifugation
130000
-
cytoplasmic form, gel filtration
137000
-
in presence of substrate, gel filtration
171000
-
gel filtration
280000
39930
E365Term mutant, theoretical
40000
E365Term mutant, determined by SDS-PAGE
50000
-
x * 50000, SDS-PAGE, ultracentrifugation of the enzyme denatured in 6 M guanidine hydrochloride and 0.1 M 2-mercaptoethanol
56000
wild-type enzyme, determined by SDS-PAGE
56030
wild-type enzyme, theoretical
60000
-
2 * 60000, SDS-PAGE
62300
2 * 62300, calculated from nucleotide sequence
63100
2 * 63100, calculated, isoform IPMS1 with His-tag
64100
65000
67000
-
2 * 67000, SDS-PAGE
68416
-
x * 68416, isoenzyme I, calculation from nucleotide sequence
72000
-
2 * 72000, SDS-PAGE
74000
alpha-IPMS-2CR, determined by SDS-PAGE
80000
-
2 * 80000, SDS-PAGE
99000
alpha-IPMS-14CR, determined by SDS-PAGE