Protein Variants | Comment | Organism |
---|---|---|
D444A | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Mycobacterium tuberculosis |
D444R | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Mycobacterium tuberculosis |
D444Y | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Mycobacterium tuberculosis |
R97A | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-leucine | non-competitive inhibition | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
3-methyl-2-oxobutanoate | mutant enzyme D444A, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
0.008 | - |
3-methyl-2-oxobutanoate | mutant enzyme R97A, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
0.009 | - |
3-methyl-2-oxobutanoate | mutant enzyme D444R, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
0.015 | - |
3-methyl-2-oxobutanoate | wild type enzyme, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
0.016 | - |
3-methyl-2-oxobutanoate | mutant enzyme D444Y, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
0.027 | - |
acetyl-CoA | mutant enzyme D444R, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
0.042 | - |
acetyl-CoA | mutant enzyme D444A, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
0.042 | - |
acetyl-CoA | mutant enzyme D444Y, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
0.14 | - |
acetyl-CoA | wild type enzyme, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
0.21 | - |
acetyl-CoA | mutant enzyme R97A, at pH 7.5 and 25°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Mycobacterium tuberculosis | - |
(2S)-2-isopropylmalate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WQB3 | - |
- |
Purification (Comment) | Organism |
---|---|
TALON affinity resin column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | - |
Mycobacterium tuberculosis | (2S)-2-isopropylmalate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
alpha-IPMS | - |
Mycobacterium tuberculosis |
Alpha-isopropylmalate synthase | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
3-methyl-2-oxobutanoate | mutant enzyme D444A, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
3.4 | - |
3-methyl-2-oxobutanoate | mutant enzyme D444Y, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
3.7 | - |
3-methyl-2-oxobutanoate | wild type enzyme, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
5.7 | - |
3-methyl-2-oxobutanoate | mutant enzyme D444R, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
7 | - |
3-methyl-2-oxobutanoate | mutant enzyme R97A, at pH 7.5 and 25°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
27 | - |
acetyl-CoA | wild type enzyme, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
33 | - |
acetyl-CoA | mutant enzyme R97A, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
70 | - |
acetyl-CoA | mutant enzyme D444A, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
80 | - |
acetyl-CoA | mutant enzyme D444Y, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
210 | - |
acetyl-CoA | mutant enzyme D444R, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
210 | - |
3-methyl-2-oxobutanoate | mutant enzyme D444Y, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
250 | - |
3-methyl-2-oxobutanoate | wild type enzyme, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
600 | - |
3-methyl-2-oxobutanoate | mutant enzyme D444A, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
630 | - |
3-methyl-2-oxobutanoate | mutant enzyme D444R, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
900 | - |
3-methyl-2-oxobutanoate | mutant enzyme R97A, at pH 7.5 and 25°C | Mycobacterium tuberculosis |