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2.1.1.107: uroporphyrinogen-III C-methyltransferase

This is an abbreviated version!
For detailed information about uroporphyrinogen-III C-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.107

Reaction

2 S-adenosyl-L-methionine +

uroporphyrinogen III
= 2 S-adenosyl-L-homocysteine +
precorrin-2

Synonyms

adenosylmethionine-uroporphyrinogen III methyltransferase, At5g40850, CobA, CobA/HemD, CysG, HemD-CobA, NirE, S-adenosyl-L-methionine dependent uroporphyrinogen III methylase, S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase, S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase, S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase, SAM-dependent uroporphyrinogen III methyltransferase, sirohaem synthase, SUMT, UMT, UPM1, UroM, uroporphyrinogen III C-methyltransferase, uroporphyrinogen III methylase, uroporphyrinogen III methyltransferase, uroporphyrinogen III methyltransferase/synthase, uroporphyrinogen III synthase/methyltransferase, uroporphyrinogen methyltransferase,

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.107 uroporphyrinogen-III C-methyltransferase

Cloned

Cloned on EC 2.1.1.107 - uroporphyrinogen-III C-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
50 fold increase of enzyme activity when overexpressed
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complementation experiments with cysteine autotroph strain
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cysG containing plasmid transformed into Escherichia coli CBK103
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DNA and amino acid sequence determination and analysis, the uroporphyrinogen III synthase is naturally fused with the methyltransferase, bypassing the need for uroporphyrinogen III decarboxylase activity, overview. Expression of cobA/hemD and isolate hemD or cobA in Escherichia coli strain BL21(DE3)
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EC 2.1.1.107 deficient mutants complemented with cysG from Salmonella typhimurium and Escherichia coli and cobA from Pseudomonas denitrificans, overexpression of yeast gene product in Escherichia coli
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Escherichia coli JM109 used for cloning all recombinant plasmids and expressing the pUC18-derived plasmid, Escherichia coli strain S13009 and BL21(DE3) used for expressing the pQE-derived plasmids and the pET-derived plasmid
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expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli DH5alpha, BL21(DE3) and RosettaT (DE3) cells
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expressed in Escherichia coli Rosetta (DE3) cells
expression in Escherichia coli
expression in Escherichia coli and Pseudomonas denitrificans
expression of 5 open reading frames, codon 1120-1959 (cobA) encodes EC 2.1.1.107 activity
gene cobA, expression in Escherichia coli strain JM109(DE3), the recombinant enzyme renders the bacteria morre resistant to potassium tellurite and potassium tellurate
in Escherichia coli CBK103
-
in Escherichia coli CBK103, amino acids 202-457 responsible for EC 2.1.1.107 activity
Q96532
in Escherichia coli JM83 and MV1184
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overexpression of cobA gene in Escherichia coli TB1
overexpression of cysG gene
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recombinant expression of C-terminally His-tagged NirE in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH10B
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