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Literature summary for 2.1.1.107 extracted from

  • Fan, J.; Wang, D.; Liang, Z.; Guo, M.; Teng, M.; Niu, L.
    Maize uroporphyrinogen III methyltransferase: overexpression of the functional gene fragments in Escherichia coli and one-step purification (2006), Protein Expr. Purif., 46, 40-46.
    View publication on PubMed
Search for more literature for 2.1.1.107

Application

Application Comment Organism
analysis red fluorescent compounds are associated with the recombinant mature SUMT which are identified as sirohydrochlorin and trimethylpyrrocorphin by spectroscopic analysis, slightly alter the protein secondary structure Zea mays

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli JM109 used for cloning all recombinant plasmids and expressing the pUC18-derived plasmid, Escherichia coli strain S13009 and BL21(DE3) used for expressing the pQE-derived plasmids and the pET-derived plasmid Zea mays

Protein Variants

Protein Variants Comment Organism
additional information deletion mutants, truncated one deleting the C-terminal extra 52 amino acids actively expressed in Escherichia coli, the mature SUMT fusion mutant or the mature SUMT deleting the N-terminal 36 amino acids including glycine-rich region involved directly in SAM binding expressed as an inclusion body in Escherichia coli Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 2 * 34000, SDS-PAGE Zea mays
52000
-
 gel filtration Zea mays
79000
-
 dynamic light scattering assay Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by Ni–NTA column Zea mays

Subunits

Subunits Comment Organism
homodimer 2 * 34000, SDS-PAGE Zea mays

Synonyms

Synonyms Comment Organism
S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase
-
 Zea mays
SUMT
-
 Zea mays
uroporphyrinogen III methyltransferase
-
 Zea mays