Literature summary for 2.1.1.107 extracted from
Woodcock, S.C.; Raux, E.; Levillayer, F.; Thermes, C.; Rambach, A.; Warren, M.J.
Effect of mutations in the transmethylase and dehydrogenase/chelatase domains of siroheme synthase (CysG) on siroheme and cobalamin biosynthesis (1998), Biochem. J., 330, 121-129.
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
complementation experiments with cysteine autotroph strain |
Escherichia coli |
Protein Variants
Protein Variants |
Comment |
Organism |
D227A |
full activity |
Escherichia coli |
D248A |
no transmethylase activity |
Escherichia coli |
D303A |
full activity |
Escherichia coli |
G224A |
unable to bind S-adenosyl-L-methionine |
Escherichia coli |
K270I |
full activity |
Escherichia coli |
R298L |
unable to bind S-adenosyl-L-methionine |
Escherichia coli |
R309L |
no transmethylase activity |
Escherichia coli |
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
2 S-adenosyl-L-methionine + uroporphyrinogen III |
Escherichia coli |
involved in biosynthesis of cobalamin |
2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ |
product is precorrin-2 |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Escherichia coli |
- |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
- |
Escherichia coli |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
2 S-adenosyl-L-methionine + uroporphyrinogen III |
methylates positions 2 and 7 |
Escherichia coli |
2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ |
product is precorrin-2 |
? |
|
2 S-adenosyl-L-methionine + uroporphyrinogen III |
involved in biosynthesis of cobalamin |
Escherichia coli |
2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ |
product is precorrin-2 |
? |
|