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1.7.2.1: nitrite reductase (NO-forming)

This is an abbreviated version!
For detailed information about nitrite reductase (NO-forming), go to the full flat file.

Word Map on EC 1.7.2.1

Reaction

nitric oxide
+
H2O
 +
ferricytochrome c
=
nitrite
 +
ferrocytochrome c
 + 2 H+

Synonyms

AcNIR, AfNiR, AniA, AxNiR, BRAO375_2740002, C551-O2 oxidoreductase, cd1 nitrite reductase, cd1NiR, cNOR, copper-containing dissimilatory nitrite reductase, copper-containing nitrite reductase, Cu-NIR, Cu-NirK, CuNIR, cytochrome c-551:O2, NO2- oxidoreductase, cytochrome cd, cytochrome cd1 nitrite reductase, cytochrome oxidase, dissimilatory nitrite reductase, dissimilatory nitrite reductase cytochrome cd1, EC 1.6.6.5, EC 1.7.99.3, EC 1.9.3.2, GK0767, GtNiR, HdNIR, hemoglobin, HydNIR, HYPDE_25578, KSU1_D0929, mARC1, mARC2, mitochondrial amidoxime reducing component, MRA2164, NiR, NiR-Pa, NirK, NirS, nitrite reductase, oxidase, Pseudomonas cytochrome, PaNiR, PNR, Pseudomonas cytochrome oxidase, PsNiR, reductase, nitrite (cytochrome), Rpic_4015, S58_68210

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.2 With a cytochrome as acceptor
                1.7.2.1 nitrite reductase (NO-forming)

Purification

Purification on EC 1.7.2.1 - nitrite reductase (NO-forming)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, CM-Sephadex, DEAE-Sephadex A-50, Sephacryl S-200, Sephadex G-50, Resource Q, Poros PE/M
-
butyl-toyopearl, Sepharose CL-6B, Sephacryl S-300, octyl-sepharose, hydroxyapatite
-
CM-cellulose, Sephadex G-150, hydroxyapatite
-
DEAE-Biogel A, TSK DEAE-5PW
-
DEAE-Sepharose, Poros HP2, Superdex 200
-
DEAE-Toyopearl, CM-cellulose, Sephacryl S-200
-
f. sp. denitrificans
-
His60 Ni Superflow resin column chromatography and Superdex 200 gel filtration
Mono S, alkyl-superose, Sephacryl S-300, Mono Q, Mono S
-
monomeric enzyme prepared by controlled succinylation of the native dimer
-
purified NiR exhibits a blue color spectrum showing maximum absorbance at 594 nm
recombinant enzyme
recombinant enzyme, contains only the c heme
-
recombinant soluble domain
recombinant wild-type and D92E and D92N mutant enzyme
-
recombinant wild-type and H139A mutant enzyme
-
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, hydrophobic interaction chromatography, dialysis, and anion exchange chromatography, followed by gel filtration
recombinant wild-type and Y10F mutant enzyme
-
recombinant wild-type enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography, the tag is removed by thrombin, followed by anion exchange chromatography
recombinant wild-type, H327A and H369A mutant enzyme
soluble fraction, DEAE-cellulose, Source 15Q, Sephadex 75
-