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Literature summary for 1.7.2.1 extracted from

  • Pinho, D.; Besson, S.; Brondino, C.D.; de Castro, B.; Moura, I.
    Copper-containing nitrite reductase from Pseudomonas chlororaphis DSM 50135 (2004), Eur. J. Biochem., 271, 2361-2369.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
copper 3.2 copper ions per trimer, most probably 3 type 1 and 3 type 2 copper, respectively Pseudomonas chlororaphis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37700
-
3 * 37700, SDS-PAGE Pseudomonas chlororaphis
107000
-
gel filtration Pseudomonas chlororaphis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrite + electron donor Pseudomonas chlororaphis
-
NO + oxidized electron donor
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas chlororaphis
-
DSM 50135
-

Purification (Commentary)

Purification (Comment) Organism
soluble fraction, DEAE-cellulose, Source 15Q, Sephadex 75 Pseudomonas chlororaphis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.33
-
electron donor azurin Pseudomonas chlororaphis
130
-
electron donor benzyl viologen Pseudomonas chlororaphis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrite + electron donor
-
Pseudomonas chlororaphis NO + oxidized electron donor
-
?
nitrite + reduced azurin azurin purified from Pseudomonas chlororaphis Pseudomonas chlororaphis NO + oxidized azurin
-
?
nitrite + reduced benzyl viologen
-
Pseudomonas chlororaphis NO + oxidized benzyl viologen
-
?

Subunits

Subunits Comment Organism
trimer 3 * 37700, SDS-PAGE Pseudomonas chlororaphis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.62
-
nitrite pH 7.0, electron donor azurin Pseudomonas chlororaphis
243
-
nitrite pH 7.0, electron donor benzyl viologen Pseudomonas chlororaphis

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas chlororaphis isoelectric focusing 6.2 5.4