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Literature summary for 1.7.2.1 extracted from

  • Michalski, W.P.; Nicholas, D.J.D.
    Molecular characterization of a copper-containing nitrite reductase from Rhodopseudomonas sphaereoides forma sp. denitrificans (1985), Biochim. Biophys. Acta, 828, 130-137.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, complete inhibition after preincubation for 10 min Cereibacter sphaeroides
KCN
-
Cereibacter sphaeroides
Urea 4 M, 60% inhibition Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0357
-
nitrite
-
Cereibacter sphaeroides

Metals/Ions

Metals/Ions Comment Organism Structure
copper one subunit contains a type 1 copper center, the second subunit a type 2 copper center, activity of copper depleted enzyme can be restored Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
gel filtration, gradient PAGE Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
f. sp. denitrificans
-

Purification (Commentary)

Purification (Comment) Organism
f. sp. denitrificans Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40
-
if reduced nitrite is measured Cereibacter sphaeroides
80 90 if oxidized benzyl viologen is measured Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N-dimethyl-p-phenylenediamine + oxidized benzyl viologen
-
Cereibacter sphaeroides ?
-
?
nitrite + ferrocytochrome c artificial electron donor: reduced benzyl viologen Cereibacter sphaeroides nitric oxide + H2O + ferricytochrome c
-
?
O2-. + H+ purified enzyme shows superoxide dismutase activity, approx. one-third that of pure superoxide dismutase Cereibacter sphaeroides H2O2 + O2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 39000-42000, enzyme composed of 2 nonidentical subunits: one contains Cu2+ type 1, the second subunit contains Cu2+ type 2, SDS-PAGE Cereibacter sphaeroides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
activity is not affected after treatment for 10 min, first step in purification Cereibacter sphaeroides