Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, complete inhibition after preincubation for 10 min | Cereibacter sphaeroides | |
KCN | - |
Cereibacter sphaeroides | |
Urea | 4 M, 60% inhibition | Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0357 | - |
nitrite | - |
Cereibacter sphaeroides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | one subunit contains a type 1 copper center, the second subunit a type 2 copper center, activity of copper depleted enzyme can be restored | Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration, gradient PAGE | Cereibacter sphaeroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
f. sp. denitrificans | - |
Purification (Comment) | Organism |
---|---|
f. sp. denitrificans | Cereibacter sphaeroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40 | - |
if reduced nitrite is measured | Cereibacter sphaeroides |
80 | 90 | if oxidized benzyl viologen is measured | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N-dimethyl-p-phenylenediamine + oxidized benzyl viologen | - |
Cereibacter sphaeroides | ? | - |
? | |
nitrite + ferrocytochrome c | artificial electron donor: reduced benzyl viologen | Cereibacter sphaeroides | nitric oxide + H2O + ferricytochrome c | - |
? | |
O2-. + H+ | purified enzyme shows superoxide dismutase activity, approx. one-third that of pure superoxide dismutase | Cereibacter sphaeroides | H2O2 + O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 39000-42000, enzyme composed of 2 nonidentical subunits: one contains Cu2+ type 1, the second subunit contains Cu2+ type 2, SDS-PAGE | Cereibacter sphaeroides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
activity is not affected after treatment for 10 min, first step in purification | Cereibacter sphaeroides |