Literature summary for 1.7.2.1 extracted from

Deligeer; Fukunaga, R.; Kataoka, K.; Yamaguchi, K.; Kobayashi, K.; Tagawa, S.; Suzuki, S.
Spectroscopic and functional characterization of Cu-containing nitrite reductase from Hyphomicrobium denitrificans A3151 (2002), J. Inorg. Biochem., 91, 132-138.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.62	-	nitrite	25°C, pH 5.5	Hyphomicrobium denitrificans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
copper	2.6 atoms copper per subunit, 2 type 1 Cu and 0.6 type 2 Cu	Hyphomicrobium denitrificans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	8 * 50000, SDS-PAGE	Hyphomicrobium denitrificans
400000	-	gel filtration	Hyphomicrobium denitrificans

Organism

Organism	UniProt	Comment	Textmining
Hyphomicrobium denitrificans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate, CM-Sephadex, DEAE-Sephadex A-50, Sephacryl S-200, Sephadex G-50, Resource Q, Poros PE/M	Hyphomicrobium denitrificans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nitrite + reduced benzyl viologen	-	Hyphomicrobium denitrificans	NO + H2O + oxidized benzyl viologen	-	?

Subunits

Subunits	Comment	Organism
octamer	8 * 50000, SDS-PAGE	Hyphomicrobium denitrificans

Synonyms

Synonyms	Comment	Organism
HydNIR	-	Hyphomicrobium denitrificans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
320	-	nitrite	25°C, pH 5.5	Hyphomicrobium denitrificans

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Release 2023.1 (January 2023)