Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.7.2.1 extracted from

  • Masuko, M.; Iwasaki, H.; Sakurai, T.; Suzuki, S.; Nakahara, A.
    Characterization of nitrite reductase from a denitrifier, Alcaligenes sp. NCIB 11015. A novel copper protein (1984), J. Biochem., 96, 447-454.
    View publication on PubMed
Search for more literature for 1.7.2.1

Metals/Ions

Metals/Ions Comment Organism Structure
copper contains 2 type 1 copper atoms per molecule but no other types of copper Alcaligenes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 2 * 37000, SDS-PAGE Alcaligenes sp.

Organism

Organism UniProt Comment Textmining
Alcaligenes sp.
-
 NCIB 11015
-

Purification (Commentary)

Purification (Comment) Organism
CM-cellulose, Sephadex G-150, hydroxyapatite Alcaligenes sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.1
-
-
 Alcaligenes sp.
23.4
-
-
 Alcaligenes sp.
117
-
 after storage at -20°C for 25 h and thawing in a water bath at 20°C Alcaligenes sp.

Storage Stability

Storage Stability Organism
0°C, 3 weeks, no loss of activity Alcaligenes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrite + ferrocytochrome c
-
 Alcaligenes sp. nitric oxide + H2O + ferricytochrome c
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 37000, SDS-PAGE Alcaligenes sp.