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0.143 - 272
3-dehydroquinate
0.07 - 1700
3-dehydroshikimate
0.143
3-dehydroquinate
pH 7.0, 30°C
272
3-dehydroquinate
pH 7.0, 30°C
0.07
3-dehydroshikimate
pH 10.5, 30°C, EcDQD/SDH2, gallate formation
0.099
3-dehydroshikimate
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHd, with NADPH
0.304
3-dehydroshikimate
pH 10.5, 30°C, EcDQD/SDH2, gallate formation
0.508
3-dehydroshikimate
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with NADPH
1.411
3-dehydroshikimate
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with NADPH
4.16
3-dehydroshikimate
pH 7.0, 30°C, EcDQD/SDH3, DQD activity
5.32
3-dehydroshikimate
pH 8.5, temperature not specified in the publication
5.32
3-dehydroshikimate
pH 8.5, 30°C, EcDQD/SDH2, shikimate formation
9.6
3-dehydroshikimate
mutant K69A, pH 7.3, 25°C
65.6
3-dehydroshikimate
pH 6.5, 30°C, EcDQD/SDH3, shikimate formation
65.6
3-dehydroshikimate
pH 6.5, temperature not specified in the publication
96
3-dehydroshikimate
pH 7.0, 30°C, EcDQD/SDH2, DQD activity
134.5
3-dehydroshikimate
pH 7.0, 30°C, EcDQD/SDH1, DQD activity
186
3-dehydroshikimate
pH 7.0, 30°C, recombinant isozyme VvSDH3
435
3-dehydroshikimate
pH 9.0, 30°C
611
3-dehydroshikimate
pH 7.0, 30°C, recombinant isozyme VvSDH1
805
3-dehydroshikimate
pH 6.5, temperature not specified in the publication
805.8
3-dehydroshikimate
pH 6.5, 30°C, EcDQD/SDH1, shikimate formation
1650
3-dehydroshikimate
pH 7.0, 30°C
1700
3-dehydroshikimate
wild-type enzyme, pH 7.3, 25°C
0.014
L-quinate
pH and temperature not specified in the publication, mutant T381S
0.022
L-quinate
pH and temperature not specified in the publication, mutant T381A
1.64
L-quinate
pH and temperature not specified in the publication, mutant S338G/T381G
2.64
L-quinate
pH and temperature not specified in the publication, mutant T381G
1.42
NAD+
pH 9.0, 25°C, recombinant wild-type enzyme
1.8
NAD+
pH 9.0, 25°C, recombinant mutant N149D
4.17
NAD+
pH 9.0, 25°C, recombinant mutant N149D/V152F
12.55
NAD+
pH 9.0, 25°C, recombinant mutant S131A/N149D/V152F
16.72
NAD+
pH 9.0, 25°C, recombinant mutant S131A/L135A/N149D/V152F
0.13
NADP+
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHD, with shikimate
0.322
NADP+
pH 9.0, 25°C, recombinant mutant N149D
0.374
NADP+
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with shikimate
0.457
NADP+
pH 9.0, 25°C, recombinant mutant N149D/V152F
0.848
NADP+
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with shikimate
2.217
NADP+
pH 9.0, 25°C, recombinant mutant S131A/N149D/V152F
3.133
NADP+
pH 9.0, 25°C, recombinant mutant S131A/L135A/N149D/V152F
262
NADP+
pH 7.0, 30°C, recombinant isozyme VvSDH1
537
NADP+
pH 9.0, 30°C, recombinant isozyme VvSDH4
680
NADP+
pH 9.0, 30°C, recombinant isozyme VvSDH3
862
NADP+
pH 7.0, 30°C, recombinant isozyme VvSDH3
1113
NADP+
pH 9.0, 30°C, recombinant isozyme VvSDH1
1690
NADP+
pH 9.0, temperature not specified in the publication
1691.6
NADP+
pH 9.0, 30°C, EcDQD/SDH3, shikimate oxidation
2070
NADP+
pH 9.0, temperature not specified in the publication
2070.6
NADP+
pH 9.0, 30°C, EcDQD/SDH2, shikimate oxidation
3200
NADP+
pH 9.0, 25°C, recombinant wild-type enzyme
3430
NADP+
-
pH not specified in the publication, 25°C
14000
NADP+
pH 9.0, temperature not specified in the publication
14017.7
NADP+
pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation
0.195
NADPH
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with 3-dehydroshikimate
0.197
NADPH
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHD, with 3-dehydroshikimate
0.748
NADPH
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with 3-dehydroshikimate
24
NADPH
mutant K69A, pH 7.3, 25°C
3207.8
NADPH
pH 6.5, 30°C, EcDQD/SDH3, shikimate formation
3210
NADPH
pH 6.5, temperature not specified in the publication
4500
NADPH
wild-type enzyme, pH 7.3, 25°C
6300
NADPH
pH 8.5, temperature not specified in the publication
6301.4
NADPH
pH 8.5, 30°C, EcDQD/SDH2, shikimate formation
7858.6
NADPH
pH 6.5, 30°C, EcDQD/SDH1, shikimate formation
7860
NADPH
pH 6.5, temperature not specified in the publication
0.139
shikimate
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHD, with NADP+
0.535
shikimate
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with NADP+
0.56
shikimate
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with NADP+
4.7
shikimate
pH and temperature not specified in the publication, mutant T381A
14.9
shikimate
pH and temperature not specified in the publication, mutant T381G
18
shikimate
pH 7.0, 30°C, recombinant isozyme VvSDH3
22.17
shikimate
pH 9.0, 30°C, EcDQD/SDH3, shikimate oxidation
22.2
shikimate
pH 9.0, temperature not specified in the publication
24.9
shikimate
pH and temperature not specified in the publication, mutant S338G/T381G
71.2
shikimate
pH 9.0, 30°C, EcDQD/SDH2, shikimate oxidation
71.3
shikimate
pH 9.0, temperature not specified in the publication
76
shikimate
pH 7.0, 30°C, recombinant isozyme VvSDH1
111.8
shikimate
pH and temperature not specified in the publication, mutant T381S
168
shikimate
pH 7.0, 30°C
178
shikimate
pH 9.0, 30°C, recombinant isozyme VvSDH4
189
shikimate
pH 9.0, 30°C, recombinant isozyme VvSDH3
779.2
shikimate
pH and temperature not specified in the publication, mutant S338G
854.3
shikimate
pH and temperature not specified in the publication, wild-type enzyme
1051
shikimate
pH 9.0, 30°C, recombinant isozyme VvSDH1
1461.5
shikimate
pH 9.0, 25°C, recombinant wild-type enzyme
1670
shikimate
pH 9.0, 30°C
3620
shikimate
-
pH not specified in the publication, 25°C
23159.5
shikimate
pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation
23200
shikimate
pH 9.0, temperature not specified in the publication