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0.36 - 114
3-dehydroquinate
0.046 - 329
3-dehydroshikimate
additional information
additional information
-
0.36
3-dehydroquinate
pH 7.0, 30°C
114
3-dehydroquinate
pH 7.0, 30°C
0.046
3-dehydroshikimate
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHd, with NADPH
0.078
3-dehydroshikimate
25°C, pH 7.3
0.168
3-dehydroshikimate
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with NADPH
0.405
3-dehydroshikimate
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with NADPH
0.73
3-dehydroshikimate
mutant K69A, pH 7.3, 25°C
2.14
3-dehydroshikimate
pH 10.5, 30°C, EcDQD/SDH2, gallate formation
2.87
3-dehydroshikimate
pH 10.5, 30°C, EcDQD/SDH2, gallate formation
3.67
3-dehydroshikimate
pH 7.0, 30°C, EcDQD/SDH1, DQD activity
8.62
3-dehydroshikimate
pH 7.0, 30°C, recombinant isozyme VvSDH3
30.9
3-dehydroshikimate
pH 7.0, 30°C, EcDQD/SDH1, DQD activity
47
3-dehydroshikimate
pH 8.5, temperature not specified in the publication
47
3-dehydroshikimate
pH 8.5, 30°C, EcDQD/SDH1, shikimate formation
49
3-dehydroshikimate
pH 7.0, 25°C, recombinant AroE
49
3-dehydroshikimate
-
pH 7.0, 25°C, with NADPH
50
3-dehydroshikimate
wild-type enzyme, pH 7.3, 25°C
57.6
3-dehydroshikimate
pH 6.5, temperature not specified in the publication
57.6
3-dehydroshikimate
pH 6.5, 30°C, EcDQD/SDH1, shikimate formation
60.4
3-dehydroshikimate
pH 7.0, 30°C, EcDQD/SDH1, DQD activity
95.85
3-dehydroshikimate
pH 7.0, 30°C, recombinant isozyme VvSDH1
118
3-dehydroshikimate
pH 9.0, 30°C
118
3-dehydroshikimate
with NADPH, pH 8.6, 30°C
307
3-dehydroshikimate
pH 6.5, temperature not specified in the publication
307
3-dehydroshikimate
pH 6.5, 30°C, EcDQD/SDH1, shikimate formation
329
3-dehydroshikimate
pH 7.0, 30°C
0.062
L-quinate
pH and temperature not specified in the publication, mutant T381S
0.113
L-quinate
pH and temperature not specified in the publication, mutant T381A
6.7
L-quinate
pH and temperature not specified in the publication, mutant S338G/T381G
8.8
L-quinate
pH and temperature not specified in the publication, mutant T381G
5.2
NAD+
pH 8.0, 60°C, recombinant enzyme
6.72
NAD+
pH 8.8, presence of 2 mM shikimate
6.72
NAD+
in the presence of shikimate
40.7
NAD+
pH 8.8, presence of 2 mM quinate
61.1
NAD+
pH 8.8, presence of 2 mM shikimate
97.1
NAD+
pH 8.8, presence of 2 mM shikimate
97.1
NAD+
in the presence of shikimate
0.006
NADP+
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHD, with shikimate
0.008
NADP+
-
pH 7.5, 22°C, mutant K385A
0.036
NADP+
-
pH 7.5, 22°C, mutant K385N/D423N
0.043
NADP+
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with shikimate
0.066
NADP+
-
pH 7.5, 22°C, mutant K385N
0.077
NADP+
in the presence of quinate
0.092
NADP+
-
pH 7.5, 22°C, mutant D423N
0.117
NADP+
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with shikimate
1.86
NADP+
-
pH 7.5, 22°C, mutant Y550A
2.77
NADP+
pH 8.8, 25°C, with shikimate
3.11
NADP+
-
pH 7.5, 22°C, mutant Y550F
4.51
NADP+
-
mutant Q78L, 22°C
5.3
NADP+
pH 8.8, presence of 2 mM shikimate
5.3
NADP+
in the presence of shikimate
5.9
NADP+
pH 7.0, 25°C, recombinant AroE
5.9
NADP+
-
pH 7.0, 25°C, with shikimate
7.1
NADP+
pH 8.0, 60°C, recombinant enzyme
7.1
NADP+
-
with shikimate, pH 8.0, 25°C
11.2
NADP+
-
pH 7.5, 22°C, mutant S338A
15.1
NADP+
-
mutant T381A, 22°C
19.78
NADP+
pH 7.0, 30°C, recombinant isozyme VvSDH3
22.8
NADP+
pH 7.0, 25°C, with shikimate
26.11
NADP+
pH 9.0, 30°C, recombinant isozyme VvSDH3
28.89
NADP+
pH 9.0, 30°C, recombinant isozyme VvSDH4
30.7
NADP+
pH 7.0, 30°C, recombinant isozyme VvSDH1
34
NADP+
-
mutant H335A, 22°C
43.3
NADP+
-
mutant T422S, 22°C
52.1
NADP+
-
mutant T407A, 22°C
52.9
NADP+
-
pH 7.5, 22°C, mutant S336A
55.5
NADP+
with shikimate, pH 9.0, 25°C
70.2
NADP+
pH 9.0, temperature not specified in the publication
70.2
NADP+
pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation
105
NADP+
-
mutant T381S, 22°C
135
NADP+
pH 9.0, temperature not specified in the publication
135
NADP+
pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation
146.2
NADP+
-
pH not specified in the publication, 25°C
146.2
NADP+
-
pH not specified in the publication, 25°C, with shikimate
237
NADP+
-
with shikimate, pH 9.0, 20°C
239
NADP+
pH 9.0, 30°C, recombinant isozyme VvSDH1
261.5
NADP+
pH 8.8, presence of 2 mM shikimate
261.5
NADP+
in the presence of shikimate
302
NADP+
pH 8.8, 25°C, with shikimate
390
NADP+
with shikimate, pH 7.3, 87°C
399
NADP+
-
pH 7.5, 22°C, wild-type DELTA88DHQ-SDH enzyme variant
399
NADP+
-
wild-type, 22°C
399
NADP+
pH 8.8, 22°C, with shikimate
399
NADP+
-
pH 9.0, 25°C, with shikimate
792
NADP+
pH 9.0, temperature not specified in the publication
792
NADP+
pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation
0.049
NADPH
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHD, with 3-dehydroshikimate
0.052
NADPH
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with 3-dehydroshikimate
0.202
NADPH
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with 3-dehydroshikimate
27.6
NADPH
pH 8.5, temperature not specified in the publication
27.6
NADPH
pH 8.5, 30°C, EcDQD/SDH1, shikimate formation
45
NADPH
pH 7.0, 25°C, recombinant AroE
45
NADPH
-
pH 7.0, 25°C, with 3-dehydroshikimate
74.1
NADPH
pH 6.5, temperature not specified in the publication
74.1
NADPH
pH 6.5, 30°C, EcDQD/SDH1, shikimate formation
389
NADPH
pH 6.5, temperature not specified in the publication
389
NADPH
pH 6.5, 30°C, EcDQD/SDH1, shikimate formation
0.16
quinate
in the presence of 2 mM NADP+
37.7
quinate
pH 8.8, presence of 2 mM NAD+
61.9
quinate
pH 9.0, 30°C
0.013
shikimate
-
pH 7.5, 22°C, mutant K385A
0.043
shikimate
-
pH 7.5, 22°C, mutant K385N/D423N
0.063
shikimate
-
pH 7.5, 22°C, mutant K385N
0.066
shikimate
mutant Y211F
0.085
shikimate
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHD, with NADP+
0.107
shikimate
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with NADP+
0.121
shikimate
-
pH 7.5, 22°C, mutant D423N
0.153
shikimate
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with NADP+
0.174
shikimate
-
mutant N406A, 22°C
0.346
shikimate
-
mutant Q582L, 22°C
1.5
shikimate
with NADP+, pH 7.0, 25°C
1.99
shikimate
pH 8.8, presence of 2 mM NAD+
1.99
shikimate
in the presence of 2 mM NAD+
2.1
shikimate
-
pH 7.5, 22°C, mutant Y550A
2.78
shikimate
pH 8.8, 25°C, with NADP+
2.78
shikimate
-
pH 8.8, temperature not specified in the publication
3.9
shikimate
-
pH 7.5, 22°C, mutant Y550F
5.16
shikimate
pH 9.0, 30°C, recombinant isozyme VvSDH3
5.8
shikimate
pH 8.8, presence of 2 mM NADP+
5.8
shikimate
in the presence of 2 mM NADP+
7.3
shikimate
-
mutant Q78L, 22°C
7.56
shikimate
pH 7.0, 30°C, recombinant isozyme VvSDH3
7.7
shikimate
pH 8.0, 60°C, recombinant enzyme
7.7
shikimate
-
with NADP+, pH 8.0, 25°C
8.2
shikimate
pH 7.0, 25°C, recombinant AroE
8.2
shikimate
-
pH 7.0, 25°C, with NADP+
11.72
shikimate
pH 7.0, 30°C, recombinant isozyme VvSDH1
11.8
shikimate
pH and temperature not specified in the publication, mutant T381A
16.8
shikimate
-
mutant T381A, 22°C
22
shikimate
pH and temperature not specified in the publication, mutant S338G/T381G
22.8
shikimate
pH 7.0, 25°C, with NADP+
24
shikimate
pH and temperature not specified in the publication, mutant T381G
26.12
shikimate
pH 9.0, 30°C, recombinant isozyme VvSDH4
31
shikimate
pH 7.0, 30°C
31.2
shikimate
pH 8.8, presence of 2 mM NAD+
31.2
shikimate
in the presence of 2 mM NAD+
33.2
shikimate
-
pH 7.5, 22°C, mutant S338A
42
shikimate
-
mutant H335A, 22°C
46.3
shikimate
-
mutant T422S, 22°C
55
shikimate
25°C, pH 9.0
55.5
shikimate
with NADP+, pH 9.0, 25°C
55.7
shikimate
pH 8.8, presence of 2 mM NAD+
66.6
shikimate
-
mutant T407A, 22°C
80.7
shikimate
pH 9.0, temperature not specified in the publication
80.7
shikimate
pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation
85.2
shikimate
pH 10.0, 30°C
94.55
shikimate
pH 9.0, 30°C, recombinant isozyme VvSDH1
114
shikimate
-
mutant T381S, 22°C
135.8
shikimate
-
pH not specified in the publication, 25°C
135.8
shikimate
-
pH not specified in the publication, 25°C, with NADP+
140
shikimate
-
pH 7.5, 22°C, mutant S336A
146
shikimate
pH 9.0, temperature not specified in the publication
146
shikimate
pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation
172
shikimate
pH and temperature not specified in the publication, mutant T381S
190
shikimate
pH 9.0, 25°C, recombinant wild-type enzyme
234
shikimate
pH 9.0, 30°C
234
shikimate
with NADP+, pH 9.0, 30°C
237
shikimate
-
with NADP+, pH 9.0, 20°C
266.2
shikimate
pH 8.8, presence of 2 mM NADP+
266.2
shikimate
in the presence of 2 mM NADP+
307
shikimate
pH 8.8, 25°C, with NADP+
399
shikimate
-
pH 9.0, 25°C
399
shikimate
-
pH 9.0, 25°C, with NADP+
427
shikimate
pH and temperature not specified in the publication, mutant S338G
428
shikimate
-
pH 7.5, 22°C, wild-type DELTA88DHQ-SDH enzyme variant
428
shikimate
-
wild-type, 22°C
428
shikimate
pH 8.8, 22°C, with NADP+
516
shikimate
pH and temperature not specified in the publication, wild-type enzyme
755
shikimate
pH 9.0, temperature not specified in the publication
755
shikimate
pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation
additional information
additional information
no measurable activity with NAD+
-
additional information
additional information
no measurable activity with NAD+
-
additional information
additional information
no measurable activity with NAD+
-
additional information
additional information
no measurable activity with NAD+
-
additional information
additional information
no measurable activity with NAD+
-
additional information
additional information
no measurable activity with NADH
-
additional information
additional information
no measurable activity with NADH
-
additional information
additional information
no measurable activity with NADH
-
additional information
additional information
no measurable activity with NADH
-
additional information
additional information
no measurable activity with NADH
-
additional information
additional information
no measurable activity with NADH+
-
additional information
additional information
no measurable activity with NADH+
-
additional information
additional information
no measurable activity with NADH+
-
additional information
additional information
no measurable activity with NADH+
-
additional information
additional information
no measurable activity with NADH+
-
additional information
additional information
no measurable activity with quinate
-
additional information
additional information
no measurable activity with quinate
-
additional information
additional information
no measurable activity with quinate
-
additional information
additional information
no measurable activity with quinate
-
additional information
additional information
no measurable activity with quinate
-
additional information
additional information
no measurable activity with shikimate
-
additional information
additional information
no measurable activity with shikimate
-
additional information
additional information
no measurable activity with shikimate
-
additional information
additional information
no measurable activity with shikimate
-
additional information
additional information
no measurable activity with shikimate
-