EC Number |
Protein Variants |
Reference |
---|
1.1.1.383 | A71S |
mutant engineered to use NADH instead of NADPH |
738976 |
1.1.1.383 | A71S/R76D/S78AD/Q110V |
mutant engineered for reversal in cofactor specificity for NADH over NADPH |
-, 739528 |
1.1.1.383 | A71S/R76D/S78D/Q110A |
mutant engineered to use NADH instead of NADPH |
738976 |
1.1.1.383 | A71S/R76D/S78D/Q110V |
mutant engineered for reversal in cofactor specificity for NADH over NADPH |
739528 |
1.1.1.383 | A71S/R76D/S78D/Q110V |
mutant engineered to use NADH instead of NADPH |
738976 |
1.1.1.383 | A71S/R76D/S78D/Q110V |
strain engineered to switch the cofactor specificity fromNADPH to NADH, application in an engineered Escherichia coli strain, enabling comprehensive utilization of the biomass for biofuel applications |
737411 |
1.1.1.383 | A71S/R76D/S78D/Q110V/D146G/G185R/K433E |
mutant engineered for reversal in cofactor specificity for NADH over NADPH |
739528 |
1.1.1.383 | more |
recipe for altering the cofactor specificity of the ketol-acid reductoisomerases (KARIs). Combining results for an engineered NADH-dependent variant of Escherichia coli KARI with available KARI crystal structures and a comprehensive KARI-sequence alignment, key cofactor specificity determinants are identified and used to construct KARIs with reversed cofactor preference |
739528 |
1.1.1.383 | Q110A |
mutant engineered to use NADH instead of NADPH |
738976 |
1.1.1.383 | Q110V |
mutant engineered to use NADH instead of NADPH |
738976 |