Application | Comment | Organism |
---|---|---|
biofuel production | Recent work has demonstrated glucose to isobutanol conversion through a modified amino acid pathway in a recombinant organism. We demonstrate that an NADH-dependent pathway enables anaerobic isobutanol production at 100% theoretical yield and at higher titer and productivity than both the NADPH-dependent pathway and transhydrogenase over-expressing strain | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A71S | mutant engineered to use NADH instead of NADPH | Escherichia coli |
A71S/R76D/S78D/Q110A | mutant engineered to use NADH instead of NADPH | Escherichia coli |
A71S/R76D/S78D/Q110V | mutant engineered to use NADH instead of NADPH | Escherichia coli |
Q110A | mutant engineered to use NADH instead of NADPH | Escherichia coli |
Q110V | mutant engineered to use NADH instead of NADPH | Escherichia coli |
R76D | mutant engineered to use NADH instead of NADPH | Escherichia coli |
S78D | mutant engineered to use NADH instead of NADPH | Escherichia coli |
S78D/Q110V | mutant engineered to use NADH instead of NADPH | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
NADPH | mutant Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
0.024 | - |
NADPH | mutant Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
0.03 | - |
NADH | mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
0.04 | - |
NADPH | wild-type, pH 7.0, temperature not specified in the publication | Escherichia coli | |
0.04 | - |
NADH | mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
0.065 | - |
NADPH | mutant S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
0.065 | - |
NADH | mutant S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
0.13 | - |
NADH | mutant S78D, pH 7.0, temperature not specified in the publication | Escherichia coli | |
0.14 | - |
NADH | mutant Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
0.28 | - |
NADH | mutant Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
0.57 | - |
NADPH | mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
0.65 | - |
NADPH | mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
0.66 | - |
NADPH | mutant S78D, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.08 | - |
NADH | wild-type, pH 7.0, temperature not specified in the publication | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P05793 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-acetolactate + NADH + H+ | - |
Escherichia coli | 2,3-dihydroxy-3-methylbutanoate + NAD+ | - |
? | |
(S)-2-acetolactate + NADPH + H+ | - |
Escherichia coli | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
NADPH | mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
0.3 | - |
NADH | wild-type, pH 7.0, temperature not specified in the publication | Escherichia coli | |
0.6 | - |
NADPH | mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
2 | - |
NADH | mutant S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
2.2 | - |
NADPH | mutant S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
2.3 | - |
NADH | mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
2.5 | - |
NADPH | mutant S78D, pH 7.0, temperature not specified in the publication | Escherichia coli | |
2.6 | - |
NADH | mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
3.1 | - |
NADH | mutant Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
3.3 | - |
NADH | mutant Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
3.6 | - |
NADPH | wild-type, pH 7.0, temperature not specified in the publication | Escherichia coli | |
3.6 | - |
NADH | mutant S78D, pH 7.0, temperature not specified in the publication | Escherichia coli | |
7.2 | - |
NADPH | mutant Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
7.2 | - |
NADPH | mutant Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
NADH | wild-type, pH 7.0, temperature not specified in the publication | Escherichia coli | |
0.5 | - |
NADPH | mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1 | - |
NADPH | mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
3.8 | - |
NADPH | mutant S78D, pH 7.0, temperature not specified in the publication | Escherichia coli | |
11 | - |
NADH | mutant Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
24.8 | - |
NADH | mutant Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
27.6 | - |
NADH | mutant S78D, pH 7.0, temperature not specified in the publication | Escherichia coli | |
31.8 | - |
NADH | mutant S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
34.2 | - |
NADPH | mutant S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
60.3 | - |
NADH | mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
74 | - |
NADH | mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
87.3 | - |
NADPH | wild-type, pH 7.0, temperature not specified in the publication | Escherichia coli | |
302 | - |
NADPH | mutant Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
569 | - |
NADPH | mutant Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli |