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show all sequences of 1.1.1.383

General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH

Brinkmann-Chen, S.; Flock, T.; Cahn, J.; Snow, C.; Brustad, E.; McIntosh, J.; Meinhold, P.; Zhang, L.; Arnold, F.; Proc. Natl. Acad. Sci. USA 110, 10946-10951 (2013)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
A71S/R76D/S78AD/Q110V
mutant engineered for reversal in cofactor specificity for NADH over NADPH
Shewanella sp.
A71S/R76D/S78D/Q110V
mutant engineered for reversal in cofactor specificity for NADH over NADPH
Escherichia coli
A71S/R76D/S78D/Q110V/D146G/G185R/K433E
mutant engineered for reversal in cofactor specificity for NADH over NADPH
Escherichia coli
additional information
recipe for altering the cofactor specificity of the ketol-acid reductoisomerases (KARIs). Combining results for an engineered NADH-dependent variant of Escherichia coli KARI with available KARI crystal structures and a comprehensive KARI-sequence alignment, key cofactor specificity determinants are identified and used to construct KARIs with reversed cofactor preference
Escherichia coli
R76D/Ser78D
mutant engineered for reversal in cofactor specificity for NADH over NADPH
Shewanella sp.
S61D/S63D
mutant engineered for reversal in cofactor specificity for NADH over NADPH
Slackia exigua
S61D/S63D/I95V
mutant engineered for reversal in cofactor specificity for NADH over NADPH
Slackia exigua
V48L/R49P/K52E/S53D
mutant engineered for reversal in cofactor specificity for NADH over NADPH
Lactococcus lactis subsp. lactis
V48L/R49P/K52L/S53D
mutant engineered for reversal in cofactor specificity for NADH over NADPH
Lactococcus lactis subsp. lactis
V48L/R49P/K52L/S53D/E59K/T182S/E320K
mutant engineered for reversal in cofactor specificity for NADH over NADPH
Lactococcus lactis subsp. lactis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
0.001
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.013
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.015
-
NADH
mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.026
-
NADH
mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.03
-
NADH
mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.041
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.045
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.047
-
NADH
mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.075
-
NADH
mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
0.09
-
NADH
mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
0.108
-
NADH
mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.113
-
NADH
mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.128
-
NADH
mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.285
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.415
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
0.6
-
NADPH
mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
0.65
-
NADPH
mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.75
-
NADPH
mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.88
-
NADPH
mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
1
-
NADPH
mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
1
-
NADPH
mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
1
-
NADPH
mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
1.075
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1.18
-
NADPH
mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
1.4
-
NADPH
mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P05793
-
-
Lactococcus lactis subsp. lactis
Q02138
-
-
Lactococcus lactis subsp. lactis IL1403
Q02138
-
-
Shewanella sp.
A0KS29
-
-
Shewanella sp. ANA-3
A0KS29
-
-
Slackia exigua
D0WGK0
-
-
Slackia exigua DSM 15923
D0WGK0
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-2-acetolactate + NADH + H+
-
739528
Escherichia coli
2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(S)-2-acetolactate + NADH + H+
-
739528
Shewanella sp.
2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(S)-2-acetolactate + NADH + H+
-
739528
Slackia exigua
2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(S)-2-acetolactate + NADH + H+
-
739528
Lactococcus lactis subsp. lactis
2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(S)-2-acetolactate + NADH + H+
-
739528
Slackia exigua DSM 15923
2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(S)-2-acetolactate + NADH + H+
-
739528
Shewanella sp. ANA-3
2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(S)-2-acetolactate + NADH + H+
-
739528
Lactococcus lactis subsp. lactis IL1403
2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(S)-2-acetolactate + NADPH + H+
-
739528
Escherichia coli
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
(S)-2-acetolactate + NADPH + H+
-
739528
Shewanella sp.
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
(S)-2-acetolactate + NADPH + H+
-
739528
Slackia exigua
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
(S)-2-acetolactate + NADPH + H+
-
739528
Lactococcus lactis subsp. lactis
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
(S)-2-acetolactate + NADPH + H+
-
739528
Slackia exigua DSM 15923
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
(S)-2-acetolactate + NADPH + H+
-
739528
Shewanella sp. ANA-3
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
(S)-2-acetolactate + NADPH + H+
-
739528
Lactococcus lactis subsp. lactis IL1403
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.06
-
NADPH
mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.08
-
NADPH
mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.1
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.1
-
NADPH
mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
0.1
-
NADPH
mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.2
-
NADPH
mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.25
-
NADPH
mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.3
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.3
-
NADPH
mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
0.35
-
NADH
mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.35
-
NADPH
mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.4
-
NADH
mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.41
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.54
-
NADPH
mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.8
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.8
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.97
-
NADH
mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
1.01
-
NADH
mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
1.07
-
NADH
mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
1.1
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
1.3
-
NADH
mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
2.3
-
NADH
mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
2.4
-
NADH
mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
3.6
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
4.3
-
NADH
mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication
Escherichia coli
4.5
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
A71S/R76D/S78AD/Q110V
mutant engineered for reversal in cofactor specificity for NADH over NADPH
Shewanella sp.
A71S/R76D/S78D/Q110V
mutant engineered for reversal in cofactor specificity for NADH over NADPH
Escherichia coli
A71S/R76D/S78D/Q110V/D146G/G185R/K433E
mutant engineered for reversal in cofactor specificity for NADH over NADPH
Escherichia coli
additional information
recipe for altering the cofactor specificity of the ketol-acid reductoisomerases (KARIs). Combining results for an engineered NADH-dependent variant of Escherichia coli KARI with available KARI crystal structures and a comprehensive KARI-sequence alignment, key cofactor specificity determinants are identified and used to construct KARIs with reversed cofactor preference
Escherichia coli
R76D/Ser78D
mutant engineered for reversal in cofactor specificity for NADH over NADPH
Shewanella sp.
S61D/S63D
mutant engineered for reversal in cofactor specificity for NADH over NADPH
Slackia exigua
S61D/S63D/I95V
mutant engineered for reversal in cofactor specificity for NADH over NADPH
Slackia exigua
V48L/R49P/K52E/S53D
mutant engineered for reversal in cofactor specificity for NADH over NADPH
Lactococcus lactis subsp. lactis
V48L/R49P/K52L/S53D
mutant engineered for reversal in cofactor specificity for NADH over NADPH
Lactococcus lactis subsp. lactis
V48L/R49P/K52L/S53D/E59K/T182S/E320K
mutant engineered for reversal in cofactor specificity for NADH over NADPH
Lactococcus lactis subsp. lactis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
0.001
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.013
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.015
-
NADH
mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.026
-
NADH
mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.03
-
NADH
mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.041
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.045
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.047
-
NADH
mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.075
-
NADH
mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
0.09
-
NADH
mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
0.108
-
NADH
mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.113
-
NADH
mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.128
-
NADH
mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.285
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.415
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
0.6
-
NADPH
mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
0.65
-
NADPH
mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.75
-
NADPH
mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.88
-
NADPH
mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
1
-
NADPH
mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
1
-
NADPH
mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
1
-
NADPH
mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
1.075
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1.18
-
NADPH
mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
1.4
-
NADPH
mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-2-acetolactate + NADH + H+
-
739528
Escherichia coli
2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(S)-2-acetolactate + NADH + H+
-
739528
Shewanella sp.
2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(S)-2-acetolactate + NADH + H+
-
739528
Slackia exigua
2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(S)-2-acetolactate + NADH + H+
-
739528
Lactococcus lactis subsp. lactis
2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(S)-2-acetolactate + NADH + H+
-
739528
Slackia exigua DSM 15923
2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(S)-2-acetolactate + NADH + H+
-
739528
Shewanella sp. ANA-3
2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(S)-2-acetolactate + NADH + H+
-
739528
Lactococcus lactis subsp. lactis IL1403
2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(S)-2-acetolactate + NADPH + H+
-
739528
Escherichia coli
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
(S)-2-acetolactate + NADPH + H+
-
739528
Shewanella sp.
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
(S)-2-acetolactate + NADPH + H+
-
739528
Slackia exigua
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
(S)-2-acetolactate + NADPH + H+
-
739528
Lactococcus lactis subsp. lactis
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
(S)-2-acetolactate + NADPH + H+
-
739528
Slackia exigua DSM 15923
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
(S)-2-acetolactate + NADPH + H+
-
739528
Shewanella sp. ANA-3
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
(S)-2-acetolactate + NADPH + H+
-
739528
Lactococcus lactis subsp. lactis IL1403
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.06
-
NADPH
mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.08
-
NADPH
mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.1
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.1
-
NADPH
mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
0.1
-
NADPH
mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.2
-
NADPH
mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.25
-
NADPH
mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.3
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.3
-
NADPH
mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
0.35
-
NADH
mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.35
-
NADPH
mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.4
-
NADH
mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.41
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.54
-
NADPH
mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.8
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.8
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.97
-
NADH
mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
1.01
-
NADH
mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
1.07
-
NADH
mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
1.1
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
1.3
-
NADH
mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
2.3
-
NADH
mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
2.4
-
NADH
mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
3.6
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
4.3
-
NADH
mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication
Escherichia coli
4.5
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.05
-
NADPH
mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.08
-
NADPH
mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.11
-
NADPH
mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.17
-
NADPH
mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
0.25
-
NADPH
mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.3
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.4
-
NADPH
mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication; mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.43
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.47
-
NADPH
mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.5
-
NADPH
mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
2.6
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
2.7
-
NADH
mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
3.7
-
NADH
mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
9
-
NADH
mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication; wild-type, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
14
-
NADH
mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
22
-
NADH
mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
32
-
NADH
mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
65
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
71
-
NADH
mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
74
-
NADH
mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
88
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
165
-
NADH
mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication
Escherichia coli
800
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
4500
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.05
-
NADPH
mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.08
-
NADPH
mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.11
-
NADPH
mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.17
-
NADPH
mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
0.25
-
NADPH
mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
0.3
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.4
-
NADPH
mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication; mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.43
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.47
-
NADPH
mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
0.5
-
NADPH
mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
2.6
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
2.7
-
NADH
mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
3.7
-
NADH
mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
9
-
NADH
mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication; wild-type, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
14
-
NADH
mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
22
-
NADH
mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
32
-
NADH
mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
65
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
71
-
NADH
mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication
Lactococcus lactis subsp. lactis
74
-
NADH
mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
88
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
165
-
NADH
mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication
Escherichia coli
800
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Slackia exigua
4500
-
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
Shewanella sp.
Other publictions for EC 1.1.1.383
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737411
Wu
-
Cofactor engineering of ketol- ...
Escherichia coli
Algal Res.
19
162-167
2016
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738975
Milne
Excessive by-product formation ...
Escherichia coli
Metab. Eng. Commun.
3
39-51
2016
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
731279
Cahn
Cofactor specificity motifs an ...
Azotobacter vinelandii, Azotobacter vinelandii ATCC BAA-1303
Biochem. J.
468
475-484
2015
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
732457
Brinkmann-Chen
Uncovering rare NADH-preferrin ...
Hydrogenobaculum sp. Y04AAS1, Ignisphaera aggregans, Metallosphaera sedula
Metab. Eng.
26C
17-22
2014
-
-
3
-
-
-
-
6
-
-
-
-
-
3
-
-
3
-
-
-
-
-
6
-
-
-
-
6
3
-
-
6
-
-
-
-
-
3
6
-
-
-
-
-
-
6
-
-
-
-
-
-
-
3
-
-
-
-
6
-
-
-
-
6
3
-
-
-
-
-
-
-
6
6
739528
Brinkmann-Chen
General approach to reversing ...
Escherichia coli, Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. lactis IL1403, Shewanella sp., Shewanella sp. ANA-3, Slackia exigua, Slackia exigua DSM 15923
Proc. Natl. Acad. Sci. USA
110
10946-10951
2013
-
-
-
-
10
-
-
26
-
-
-
-
-
7
-
-
-
-
-
-
-
-
14
-
-
-
-
26
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
26
-
-
-
-
-
-
-
-
-
-
-
-
14
-
-
-
-
26
-
-
-
-
-
-
-
-
24
24
738976
Bastian
Engineered ketol-acid reductoi ...
Escherichia coli
Metab. Eng.
13
345-352
2011
-
1
-
-
8
-
-
14
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
13
-
-
-
-
-
-
-
-
1
-
-
-
8
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
13
-
-
-
-
-
-
-
-
14
14