EC Number   |
Reference |
|---|
   5.1.3.30 | DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant overexpression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) |
748499 |
| 5.1.3.30 | expressed in Escherichia coli |
727229, 727652 |
| 5.1.3.30 | expressed with a C-terminal 6His-tag in Escherichia coli |
726795 |
| 5.1.3.30 | expression in Escherichia coli |
727226, 727657, 728603, 762854 |
| 5.1.3.30 | gene CLOBOL_00069, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli |
746879 |
| 5.1.3.30 | gene dpe, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
748412 |
| 5.1.3.30 | gene dpe, recombinant food-grade overexpression of His-tagged enzyme in Bacillus pumilus using the P2 promoter, derived from a cell wall protein promoter of the host bacterium, the recombinant protein is soluble and bioactive, and is secreted to a high leve, subcloning in Escherichis coli strain DH5alphal |
748289 |
| 5.1.3.30 | gene rdpe, recombinant constitutive enzyme expression in Bacillus subtilis strains 1A751-SR, 1A751-GR, and 1A751S1-XR (from strain 1A751), in which two extracellular proteases (nprE, aprE) are deleted, Bacillus subtilis is transformed using the Paris method, screening and characterization of different inducible promoters, xylose-inducible expression system, method evaluation, detailed overview |
748350 |
| 5.1.3.30 | gene rdpe, recombinant expression in and secretion from Bacillus subtilis alanine racemase knockout (deletion of gene dal using Cre/lox system) strains 1A751D2R and 1A751D2C, construction of food-grade expression plasmids with auxotrophic marker, the food-grade plasmids pMA5-DAL-RDPE and pMA5-DAL are transformed into the food-grade host strain 1A751D2 with the deficiency of alanine racemase gene to generate the recombinant strain 1A751D2R and 1A751D2C, respectively. The extracellular activity of enzyme RDPE is gradually increased with the fermentation process, and the highest activity reaches 46 U/ml at 72 h, fed-batch fermentation, method evaluation, overview |
747303 |
| 5.1.3.30 | recombinant enzyme expression in Escherichia coli strain BL21(DE3), co-expression with D-glucose isomerase (GIase) from Acidothermus cellulolyticus from plasmid pETDuet-Dosp-DPE/Acce-GI |
748501 |
