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Literature summary for 5.1.3.30 extracted from

  • Jia, M.; Mu, W.; Chu, F.; Zhang, X.; Jiang, B.; Zhou, L.L.; Zhang, T.
    A D-psicose 3-epimerase with neutral pH optimum from Clostridium bolteae for D-psicose production: cloning, expression, purification, and characterization (2013), Appl. Microbiol. Biotechnol., 98, 717-725.
    View publication on PubMed
Search for more literature for 5.1.3.30

Application

Application Comment Organism
synthesis for the high production of D-psicose from D-fructose, the reaction reaches equilibrium after 160 min with the highest turnover yield (28.8%) observed at 55 °C Enterocloster bolteae

Cloned(Commentary)

Cloned (Comment) Organism
expressed with a C-terminal 6His-tag in Escherichia coli Enterocloster bolteae

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Enterocloster bolteae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
27.4
-
 D-psicose pH 7.0, 55°C Enterocloster bolteae
59.8
-
 D-fructose pH 7.0, 55°C Enterocloster bolteae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ metalloprotein that exhibits the highest activity in the presence of Co2+ Enterocloster bolteae
Mn2+ enhances activity Enterocloster bolteae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33015
-
 4 * 33015, calculated from sequence Enterocloster bolteae
34000
-
 4 * 34000, SDS-PAGE Enterocloster bolteae
139000
-
 gel filtration Enterocloster bolteae

Organism

Organism UniProt Comment Textmining
Enterocloster bolteae A8RG82
-
-
Enterocloster bolteae ATCC BAA-613 A8RG82
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Enterocloster bolteae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose under the optimal conditions, the equilibrium ratio of D-fructose to D-psicose is 69:31, relative activity with D-fructose is 66.4% of the activity with D-psicose Enterocloster bolteae D-psicose
-
 r
D-fructose under the optimal conditions, the equilibrium ratio of D-fructose to D-psicose is 69:31, relative activity with D-fructose is 66.4% of the activity with D-psicose Enterocloster bolteae ATCC BAA-613 D-psicose
-
 r
D-psicose under the optimal conditions, the equilibrium ratio of D-fructose to D-psicose is 69:31, relative activity with D-fructose is 66.4% of the activity with D-psicose Enterocloster bolteae D-fructose
-
 r
D-psicose under the optimal conditions, the equilibrium ratio of D-fructose to D-psicose is 69:31, relative activity with D-fructose is 66.4% of the activity with D-psicose Enterocloster bolteae ATCC BAA-613 D-fructose
-
 r
D-tagatose
-
 Enterocloster bolteae D-sorbose
-
 r
D-tagatose
-
 Enterocloster bolteae ATCC BAA-613 D-sorbose
-
 r
additional information no activity with D-fructose 6-phosphate and D-ribulose 5-phosphate. Catalytic efficiency (kcat/KM) of D-tagatose is 127fold lower than catalytic efficiency of D-psicose Enterocloster bolteae ?
-
 ?
additional information no activity with D-fructose 6-phosphate and D-ribulose 5-phosphate. Catalytic efficiency (kcat/KM) of D-tagatose is 127fold lower than catalytic efficiency of D-psicose Enterocloster bolteae ATCC BAA-613 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 34000, SDS-PAGE Enterocloster bolteae
tetramer 4 * 33015, calculated from sequence Enterocloster bolteae

Synonyms

Synonyms Comment Organism
CLOBOL_00069 locus name Enterocloster bolteae
DPEase ambiguous Enterocloster bolteae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Enterocloster bolteae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 40°C about 50% of maximal activity, 70°C: about 40% of maximal activity Enterocloster bolteae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 in the absence of Co2+, the activity is very stable below 45 °C but decreases at temperatures above 50 °C with increasing reaction times. When incubated with 0.4 mM Co2+, the half-lives (t1/2) of the enzyme are extended Enterocloster bolteae
50
-
 in the absence of Co2+, the activity is very stable below 45 °C but decreases at temperatures above 50 °C with increasing reaction times. When incubated with 0.4 mM Co2+, the half-lives (t1/2) of the enzyme are extended Enterocloster bolteae
55
-
 half-life: 156 min Enterocloster bolteae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
49
-
 D-psicose pH 7.0, 55°C Enterocloster bolteae
59
-
 D-fructose pH 7.0, 55°C Enterocloster bolteae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Enterocloster bolteae

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 pH 5.5: about 50% of maximal activity, pH 8.5: about 40% of maximal activity Enterocloster bolteae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7.5 after incubation at 4 °C for 2 h, more than 90% of the residual activity is retained at pH values ranging from of 6.0–7.5 Enterocloster bolteae

pI Value

Organism Comment pI Value Maximum pI Value
Enterocloster bolteae calculated from sequence
-
 5.02

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.99
-
 D-fructose pH 7.0, 55°C Enterocloster bolteae
1.78
-
 D-psicose pH 7.0, 55°C Enterocloster bolteae