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Literature summary for 5.1.3.30 extracted from

  • Zhang, W.; Zhang, T.; Jiang, B.; Mu, W.
    Biochemical characterization of a D-psicose 3-epimerase from Treponema primitia ZAS-1 and its application on enzymatic production of D-psicose (2016), J. Sci. Food. Agric., 96, 49-56 .
    View publication on PubMed

Application

Application Comment Organism
synthesis Trpr-DPEase might be appropriate for the industrial production of D-psicose. Comparison of D-psicose productivity of various ketose 3-epimerases, overview Treponema primitia

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant overexpression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Treponema primitia

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ inactivation at 1 mM Treponema primitia
Ca2+ inactivation at 1 mM Treponema primitia
Cu2+ inactivation at 1 mM Treponema primitia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Treponema primitia
0.209
-
D-psicose pH 8.0, 70°C, recombinant enzyme Treponema primitia
0.279
-
D-fructose pH 8.0, 70°C, recombinant enzyme Treponema primitia

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ highly activating at 1 mM, essential for the activity of the recombinant enzyme Treponema primitia
Mn2+ highly activating at 1 mM Treponema primitia
additional information the enzyme is metal-dependent Treponema primitia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-psicose Treponema primitia
-
D-fructose
-
r
D-psicose Treponema primitia ZAS-1
-
D-fructose
-
r

Organism

Organism UniProt Comment Textmining
Treponema primitia
-
-
-
Treponema primitia ZAS-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Treponema primitia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
227.5
-
purified recombinant His6-tagged enzyme, pH 8.0, 70°C Treponema primitia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose
-
Treponema primitia D-psicose
-
r
D-fructose
-
Treponema primitia ZAS-1 D-psicose
-
r
D-psicose
-
Treponema primitia D-fructose
-
r
D-psicose
-
Treponema primitia ZAS-1 D-fructose
-
r
additional information the D-psicose/D-fructose equilibrium ratio of Trpr-DPEase is 28:72. Recombinant enzyme produces D-psicose at a yield of 137.5 g/l from 500 g/l D-fructose Treponema primitia ?
-
?
additional information the D-psicose/D-fructose equilibrium ratio of Trpr-DPEase is 28:72. Recombinant enzyme produces D-psicose at a yield of 137.5 g/l from 500 g/l D-fructose Treponema primitia ZAS-1 ?
-
?

Subunits

Subunits Comment Organism
? x * 33000, recombinant enzyme, SDS-PAGE Treponema primitia

Synonyms

Synonyms Comment Organism
Trpr-DPEase
-
Treponema primitia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
recombinant enzyme Treponema primitia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
purified recombinant His6-tagged enzyme, over 75% activity remaining after 4 h Treponema primitia
50
-
purified recombinant His6-tagged enzyme, 7% activity remaining after 4 h Treponema primitia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
292.88
-
D-fructose pH 8.0, 70°C, recombinant enzyme Treponema primitia
502.77
-
D-psicose pH 8.0, 70°C, recombinant enzyme Treponema primitia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
recombinant enzyme Treponema primitia

pH Range

pH Minimum pH Maximum Comment Organism
6 9 over 80% of maximal activity within this range Treponema primitia

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 purified recombinant enzyme, 4°C, 2 h, completely stable Treponema primitia

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1049.8
-
D-fructose pH 8.0, 70°C, recombinant enzyme Treponema primitia
2405.6
-
D-psicose pH 8.0, 70°C, recombinant enzyme Treponema primitia