Application | Comment | Organism |
---|---|---|
synthesis | Trpr-DPEase might be appropriate for the industrial production of D-psicose. Comparison of D-psicose productivity of various ketose 3-epimerases, overview | Treponema primitia |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant overexpression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Treponema primitia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | inactivation at 1 mM | Treponema primitia | |
Ca2+ | inactivation at 1 mM | Treponema primitia | |
Cu2+ | inactivation at 1 mM | Treponema primitia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Treponema primitia | |
0.209 | - |
D-psicose | pH 8.0, 70°C, recombinant enzyme | Treponema primitia | |
0.279 | - |
D-fructose | pH 8.0, 70°C, recombinant enzyme | Treponema primitia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | highly activating at 1 mM, essential for the activity of the recombinant enzyme | Treponema primitia | |
Mn2+ | highly activating at 1 mM | Treponema primitia | |
additional information | the enzyme is metal-dependent | Treponema primitia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-psicose | Treponema primitia | - |
D-fructose | - |
r | |
D-psicose | Treponema primitia ZAS-1 | - |
D-fructose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Treponema primitia | - |
- |
- |
Treponema primitia ZAS-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Treponema primitia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
227.5 | - |
purified recombinant His6-tagged enzyme, pH 8.0, 70°C | Treponema primitia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose | - |
Treponema primitia | D-psicose | - |
r | |
D-fructose | - |
Treponema primitia ZAS-1 | D-psicose | - |
r | |
D-psicose | - |
Treponema primitia | D-fructose | - |
r | |
D-psicose | - |
Treponema primitia ZAS-1 | D-fructose | - |
r | |
additional information | the D-psicose/D-fructose equilibrium ratio of Trpr-DPEase is 28:72. Recombinant enzyme produces D-psicose at a yield of 137.5 g/l from 500 g/l D-fructose | Treponema primitia | ? | - |
? | |
additional information | the D-psicose/D-fructose equilibrium ratio of Trpr-DPEase is 28:72. Recombinant enzyme produces D-psicose at a yield of 137.5 g/l from 500 g/l D-fructose | Treponema primitia ZAS-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, recombinant enzyme, SDS-PAGE | Treponema primitia |
Synonyms | Comment | Organism |
---|---|---|
Trpr-DPEase | - |
Treponema primitia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
recombinant enzyme | Treponema primitia |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant His6-tagged enzyme, over 75% activity remaining after 4 h | Treponema primitia |
50 | - |
purified recombinant His6-tagged enzyme, 7% activity remaining after 4 h | Treponema primitia |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
292.88 | - |
D-fructose | pH 8.0, 70°C, recombinant enzyme | Treponema primitia | |
502.77 | - |
D-psicose | pH 8.0, 70°C, recombinant enzyme | Treponema primitia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme | Treponema primitia |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | over 80% of maximal activity within this range | Treponema primitia |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | purified recombinant enzyme, 4°C, 2 h, completely stable | Treponema primitia |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1049.8 | - |
D-fructose | pH 8.0, 70°C, recombinant enzyme | Treponema primitia | |
2405.6 | - |
D-psicose | pH 8.0, 70°C, recombinant enzyme | Treponema primitia |