Application | Comment | Organism |
---|---|---|
synthesis | the recombinant Dorea sp. DPEase displays significantly higher specific activity at acidic pHs and remarkably higher productivity of D-psicose at pH 6.0, indicating that it is appropriate for use as a different source of D-psicose producing enzyme | Dorea sp. CAG:317 |
Cloned (Comment) | Organism |
---|---|
gene dpe, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Dorea sp. CAG:317 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Dorea sp. CAG:317 | |
Ca2+ | - |
Dorea sp. CAG:317 | |
Cu2+ | - |
Dorea sp. CAG:317 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | best metal cofactor, has no effect on pH stability and slightly imporves temperature | Dorea sp. CAG:317 | |
Fe2+ | 26.59% of the activity with Co2+ at 1 mM | Dorea sp. CAG:317 | |
Mn2+ | 81.24% of the activity with Co2+ at 1 mM | Dorea sp. CAG:317 | |
additional information | metal-dependent enzyme, no activity without presence of divalent metal ion | Dorea sp. CAG:317 | |
Ni2+ | 7.96% of the activity with Co2+ at 1 mM | Dorea sp. CAG:317 | |
Zn2+ | 1.75% of the activity with Co2+ at 1 mM | Dorea sp. CAG:317 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-psicose | Dorea sp. CAG:317 | - |
D-fructose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dorea sp. CAG:317 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Dorea sp. CAG:317 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
806 | - |
purified recombinant enzyme, pH 6.0, 70°C, 1 mM Co2+ | Dorea sp. CAG:317 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose | - |
Dorea sp. CAG:317 | D-psicose | - |
r | |
D-psicose | - |
Dorea sp. CAG:317 | D-fructose | - |
r | |
additional information | the equilibrium ratio between D-fructose and D-psicose is approximately 30:70 for the recombinant enzyme, elevated temperature does not significantly shift the equilibrium toward D-psicose. The conversion ratio of D-fructose to D-psicose is calculated to be 30.1%, 31.5%, 31.8%, 31.4%, 31.1%, 31.0%, 30.4%, 29.2%, and 28.6% when the reaction temperature is 40°C, 45°C, 50°C, 55°C, 60°C, 65°C, 70°C, 75°C, and 80°C, respectively | Dorea sp. CAG:317 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 33000, SDS-PAGE | Dorea sp. CAG:317 |
Synonyms | Comment | Organism |
---|---|---|
DPEase | - |
Dorea sp. CAG:317 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Dorea sp. CAG:317 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Co2+ slightly improves the thermostability of the enzyme | Dorea sp. CAG:317 |
40 | 70 | the purified recombinant enzyme retains 70% and 30% of its initial activity after 4 h of incubation at 40°C and 50°C, respectively, 55% activity remaining after 0.5 h at 60°C, complete inactivation after 0.5 h at 70°C | Dorea sp. CAG:317 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Dorea sp. CAG:317 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | the purified recombinant enzyme retains more than 80% of initial activity when incubated for 2 h at pH values from pH 5.5 to pH 8.5 | Dorea sp. CAG:317 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.32 | - |
D-fructose | recombinant enzyme, pH 6.0, 70°C | Dorea sp. CAG:317 | |
6.9 | - |
D-psicose | recombinant enzyme, pH 6.0, 70°C | Dorea sp. CAG:317 |