Literature summary for 5.1.3.30 extracted from

Zhu, Y.; Men, Y.; Bai, W.; Li, X.; Zhang, L.; Sun, Y.; Ma, Y.
Overexpression of D-psicose 3-epimerase from Ruminococcus sp. in Escherichia coli and its potential application in D-psicose production (2012), Biotechnol. Lett., 34, 1901-1906.

Search for more literature for 5.1.3.30

Application

Application	Comment	Organism
synthesis	suitable for the industrial production of D-psicose from fructose	Ruminococcus sp.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Ruminococcus sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	leads to protein aggregation and thus drastically decreased activity	Ruminococcus sp.
Ni2+	leads to protein aggregation and thus drastically decreased activity	Ruminococcus sp.
Zn2+	leads to protein aggregation and thus drastically decreased activity	Ruminococcus sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
48	-	D-psicose	pH 8.0, 60°C	Ruminococcus sp.
216	-	D-fructose	pH 8.0, 60°C	Ruminococcus sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe3+	at 1 mM increases enzymatic activity by 30%	Ruminococcus sp.
Mg2+	at 1 mM increases enzymatic activity by 10%	Ruminococcus sp.
Mn2+	at 1 mM increases enzymatic activity by 60%	Ruminococcus sp.
additional information	activity is not dependent on the presence of metal ions	Ruminococcus sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33000	-	3 * 33000, SDS-PAGE	Ruminococcus sp.
33356	-	4 * 33356, calculated from sequence	Ruminococcus sp.
132000	-	gel filtration	Ruminococcus sp.

Organism

Organism	UniProt	Comment	Textmining
Ruminococcus sp.	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Ruminococcus sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose	the equilibrium ratio between D-psicose and D-fructose is 28:72 at 60°C	Ruminococcus sp.	D-psicose	-	r
D-psicose	the equilibrium ratio between D-psicose and D-fructose is 28:72 at 60°C	Ruminococcus sp.	D-fructose	-	r
additional information	catalytic efficiency with D-tagatose is 108fold lower than cataltic efficiency with D-psicose	Ruminococcus sp.	?	-	?

Subunits

Subunits	Comment	Organism
tetramer	3 * 33000, SDS-PAGE	Ruminococcus sp.
tetramer	4 * 33356, calculated from sequence	Ruminococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	-	Ruminococcus sp.

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
40	70	40°C: about 60% of maximal activity, 70°C: about 60% of maximal activity	Ruminococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	the thermostability of the enzyme is enhanced by the addition of Mn2+	Ruminococcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
40.45	-	D-psicose	pH 8.0, 60°C	Ruminococcus sp.
59.4	-	D-fructose	pH 8.0, 60°C	Ruminococcus sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	8	-	Ruminococcus sp.

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	9	pH 6.0: about 50% of maximal activity, pH 9.0: about 80% of maximal activity	Ruminococcus sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.275	-	D-fructose	pH 8.0, 60°C	Ruminococcus sp.
0.84	-	D-psicose	pH 8.0, 60°C	Ruminococcus sp.

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Release 2023.1 (January 2023)