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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Show all pathways known for 2.1.1.148Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.148- 637235, 659429, 660374, 681384, 719663
Show all pathways known for 2.1.1.148Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.148by nickel chromatography and gel filtration, more than 95% pure 680675
Show all pathways known for 2.1.1.148Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.148from transformed E. coli 637233
Show all pathways known for 2.1.1.148Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.148from transformed E. coli using His-tag 637232
Show all pathways known for 2.1.1.148Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.148nickel affinity and ion-exchange chromatography 694812
Show all pathways known for 2.1.1.148Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.148nickel chelating column chromatography, GSH-Sepharose column chromatography, and Superdex 200 gel filtration 720992
Show all pathways known for 2.1.1.148Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.148on Ni-NTA column 681375
Show all pathways known for 2.1.1.148Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.148to near homogeneity, by heat treatment at 85°C for 15 min, followed by Ni2+ column chromatography 678023
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