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Literature summary for 2.1.1.148 extracted from
- Kinetics and ligand-binding preferences of Mycobacterium tuberculosis thymidylate synthases, ThyA and ThyX (2008), PLoS ONE, 3, e2237.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | thymidylate synthase as a target for antitubercular drugs | Mycobacterium tuberculosis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| histidine-tagged Mycobacterium tuberculosis ThyX is overexpressed from BL21-DE3 pLysS Escherichia coli | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-fluoro-2'-deoxyuridine 5'-monophosphate | FdUMP | Mycobacterium tuberculosis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
dUMP | - |
Mycobacterium tuberculosis | |
| 0.004 | - |
5,10-methylenetetrahydrofolate | - |
Mycobacterium tuberculosis | |
| 0.047 | - |
NADPH | - |
Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 104000 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | Mycobacterium tuberculosis | - |
dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + NADPH | Mycobacterium tuberculosis | Rhodobacter capsulatus ThyX is required for de novo thymidylate synthesis | dTMP + tetrahydrofolate + NADP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel affinity and ion-exchange chromatography | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Mycobacterium tuberculosis | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + NADPH | - |
Mycobacterium tuberculosis | dTMP + tetrahydrofolate + NADP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + NADPH | Rhodobacter capsulatus ThyX is required for de novo thymidylate synthesis | Mycobacterium tuberculosis | dTMP + tetrahydrofolate + NADP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | predicted mass of a tetramer | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FDTS | - |
Mycobacterium tuberculosis |
| flavin-dependent thymidylate synthase | - |
Mycobacterium tuberculosis |
| ThyX | - |
Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Mycobacterium tuberculosis | |
| NADPH | - |
Mycobacterium tuberculosis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
5-fluoro-2'-deoxyuridine 5'-monophosphate | inhibited both ThyA and ThyX | Mycobacterium tuberculosis | |
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