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Literature summary for 2.1.1.148 extracted from

  • Kanai, A.; Sato, A.; Imoto, J.; Tomita, M.
    Archaeal Pyrococcus furiosus thymidylate synthase 1 is an RNA-binding protein (2006), Biochem. J., 393, 373-379.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
additional information autoregulates its own translation, RNA stem-loop structure acts as an inhibitory regulator of translation by preventing the binding of its Shine-Dalgarno-like sequence by positioning it in the stem region, addition of Thy1 into the in vitro translation system also inhibits translation Pyrococcus furiosus

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the recombinant C-terminal His6-tagged protein in Escherichia coli strain BL21(DE3) Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 0.5 mM enhances the activity Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
x * 29000, sequence analysis, x * 29000, SDS-PAGE Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U3C9 DSM3638
-

Purification (Commentary)

Purification (Comment) Organism
to near homogeneity, by heat treatment at 85°C for 15 min, followed by Ni2+ column chromatography Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information possesses RNA-binding activity, binds to the stem–loop RNA structure Pyrococcus furiosus ?
-
?

Subunits

Subunits Comment Organism
? x * 29000, sequence analysis, x * 29000, SDS-PAGE Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
flavin-dependent thymidylate synthase
-
Pyrococcus furiosus
Thy1
-
Pyrococcus furiosus
thymidylate synthase 1
-
Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
optimum RNA-binding activity Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
no RNA-binding activity at 95°C Pyrococcus furiosus