Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NADPH | 0.1 mM NADPH saturates the enzymatic reaction | Helicobacter pylori |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15 cells | Helicobacter pylori |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.2 M sodium chloride, 0.1 M MES, pH 6.0, and 20% (w/v) PEG2000 monomethyl ether | Helicobacter pylori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADPH | excess NADPH decreases enzyme activity | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0153 | - |
dUMP | in 50 mM Tris-HCl pH 8.0, 10 mM MgCl2, 2 mM NADPH, 0.5 mM FAD, at 22°C | Helicobacter pylori | |
0.35 | - |
5,10-methylenetetrahydrofolate | in 50 mM Tris-HCl pH 8.0, 10 mM MgCl2, 2 mM NADPH, 0.5 mM FAD, at 22°C | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
4 * 25000, SDS-PAGE | Helicobacter pylori |
25020 | - |
4 * 25020, calculated from amino acid sequence | Helicobacter pylori |
25066 | - |
4 * 25066, MALDI TOF mass spectrometry | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | Q5UVJ4 | - |
- |
Helicobacter pylori SS1 | Q5UVJ4 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel chelating column chromatography, GSH-Sepharose column chromatography, and Superdex 200 gel filtration | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Helicobacter pylori | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Helicobacter pylori SS1 | dTMP + tetrahydrofolate + FAD | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 25000, SDS-PAGE | Helicobacter pylori |
homotetramer | 4 * 25020, calculated from amino acid sequence | Helicobacter pylori |
homotetramer | 4 * 25066, MALDI TOF mass spectrometry | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
thymidylate synthase X | - |
Helicobacter pylori |
ThyX | - |
Helicobacter pylori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Helicobacter pylori |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10.5 | - |
Helicobacter pylori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme is bound to 4 FAD molecules | Helicobacter pylori |