BRENDA - Enzyme Database show
show all sequences of 2.1.1.148

Functional analysis of substrate and cofactor complex structures of a thymidylate synthase-complementing protein

Mathews, I.I.; Deacon, A.M.; Canaves, J.M.; McMullan, D.; Lesley, S.A.; Agarwalla, S.; Kuhn, P.; Structure 11, 677-690 (2003)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
NADH
required to reduce bound FAD
Dictyostelium discoideum
NADH
-
Helicobacter pylori
NADH
required to reduce bound FAD
Thermotoga maritima
NADPH
required to reduce bound FAD
Dictyostelium discoideum
NADPH
-
Helicobacter pylori
NADPH
required to reduce bound FAD
Thermotoga maritima
Application
Application
Commentary
Organism
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Bacillus anthracis
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Borreliella burgdorferi
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Campylobacter jejuni
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Chlamydia pneumoniae
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Chlamydia psittaci
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Chlamydia trachomatis
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low crossreactivity against mammalian thyA enzymes, EC 2.1.1.45
Clostridioides difficile
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low crossreactivity against mammalian thyA enzymes, EC 2.1.1.45
Clostridium botulinum
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Clostridium perfringens
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low crossreactivity against mammalian thyA enzymes, EC 2.1.1.45
Corynebacterium diphtheriae
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Ehrlichia chaffeensis
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Helicobacter pylori
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Mycobacterium avium
medicine
specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Mycobacterium leprae
medicine
specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Mycobacterium tuberculosis
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Mycobacterium tuberculosis variant bovis
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Rhodococcus sp.
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Rickettsia conorii
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Rickettsia prowazekii
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Treponema denticola
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Treponema pallidum
Cloned(Commentary)
Commentary
Organism
-
Thermotoga maritima
Crystallization (Commentary)
Crystallization
Organism
-
Thermotoga maritima
Inhibitors
Inhibitors
Commentary
Organism
Structure
FAD
-
Thermotoga maritima
HEPES
-
Thermotoga maritima
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5,10-methylenetetrahydrofolate + dUMP + FADH2
Chlamydia trachomatis
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Dictyostelium discoideum
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Mycobacterium avium
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Mycobacterium leprae
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Mycobacterium tuberculosis
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Rhodococcus sp.
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Clostridium perfringens
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Mycobacterium tuberculosis variant bovis
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Clostridium botulinum
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Clostridioides difficile
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Corynebacterium diphtheriae
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Treponema denticola
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Treponema pallidum
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Rickettsia prowazekii
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Borreliella burgdorferi
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Campylobacter jejuni
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Chlamydia pneumoniae
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Chlamydia psittaci
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Helicobacter pylori
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Thermotoga maritima
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Bacillus anthracis
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Rickettsia conorii
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Ehrlichia chaffeensis
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Thermotoga maritima DSM 3109
-
dTMP + tetrahydrofolate + FAD
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus anthracis
-
-
-
Borreliella burgdorferi
-
-
-
Campylobacter jejuni
-
-
-
Chlamydia pneumoniae
-
-
-
Chlamydia psittaci
-
-
-
Chlamydia trachomatis
-
-
-
Clostridioides difficile
-
-
-
Clostridium botulinum
-
-
-
Clostridium perfringens
-
-
-
Corynebacterium diphtheriae
-
-
-
Dictyostelium discoideum
-
-
-
Ehrlichia chaffeensis
-
-
-
Helicobacter pylori
-
-
-
Helicobacter pylori
Q9ZJ93
-
-
Mycobacterium avium
-
-
-
Mycobacterium leprae
-
; simultaneous occurrence of thyA and thyX
-
Mycobacterium tuberculosis
-
; simultaneous occurrence of thyA and thyX
-
Mycobacterium tuberculosis variant bovis
-
-
-
Rhodococcus sp.
-
-
-
Rickettsia conorii
-
-
-
Rickettsia prowazekii
-
-
-
Thermotoga maritima
Q9WYT0
; TM0449
-
Thermotoga maritima DSM 3109
Q9WYT0
TM0449
-
Treponema denticola
-
-
-
Treponema pallidum
-
-
-
Purification (Commentary)
Commentary
Organism
-
Thermotoga maritima
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Chlamydia trachomatis
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Chlamydia trachomatis
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Chlamydia trachomatis
dTMP + tetrahydrofolate + FAD
-
637235
Chlamydia trachomatis
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Dictyostelium discoideum
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Dictyostelium discoideum
dTMP + tetrahydrofolate + FAD
-
637235
Dictyostelium discoideum
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium avium
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium avium
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium avium
dTMP + tetrahydrofolate + FAD
-
637235
Mycobacterium avium
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium leprae
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium leprae
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium leprae
dTMP + tetrahydrofolate + FAD
-
637235
Mycobacterium leprae
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium tuberculosis
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium tuberculosis
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium tuberculosis
dTMP + tetrahydrofolate + FAD
-
637235
Mycobacterium tuberculosis
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Helicobacter pylori
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Rhodococcus sp.
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Rhodococcus sp.
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Rhodococcus sp.
dTMP + tetrahydrofolate + FAD
-
637235
Rhodococcus sp.
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Clostridium perfringens
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Clostridium perfringens
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Clostridium perfringens
dTMP + tetrahydrofolate + FAD
-
637235
Clostridium perfringens
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium tuberculosis variant bovis
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium tuberculosis variant bovis
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium tuberculosis variant bovis
dTMP + tetrahydrofolate + FAD
-
637235
Mycobacterium tuberculosis variant bovis
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Clostridium botulinum
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Clostridium botulinum
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Clostridium botulinum
dTMP + tetrahydrofolate + FAD
-
637235
Clostridium botulinum
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Clostridioides difficile
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Clostridioides difficile
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Clostridioides difficile
dTMP + tetrahydrofolate + FAD
-
637235
Clostridioides difficile
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Corynebacterium diphtheriae
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Corynebacterium diphtheriae
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Corynebacterium diphtheriae
dTMP + tetrahydrofolate + FAD
-
637235
Corynebacterium diphtheriae
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Treponema denticola
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Treponema denticola
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Treponema denticola
dTMP + tetrahydrofolate + FAD
-
637235
Treponema denticola
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Treponema pallidum
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Treponema pallidum
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Treponema pallidum
dTMP + tetrahydrofolate + FAD
-
637235
Treponema pallidum
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Rickettsia prowazekii
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Rickettsia prowazekii
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Rickettsia prowazekii
dTMP + tetrahydrofolate + FAD
-
637235
Rickettsia prowazekii
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Borreliella burgdorferi
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Borreliella burgdorferi
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Borreliella burgdorferi
dTMP + tetrahydrofolate + FAD
-
637235
Borreliella burgdorferi
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Campylobacter jejuni
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Campylobacter jejuni
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Campylobacter jejuni
dTMP + tetrahydrofolate + FAD
-
637235
Campylobacter jejuni
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Chlamydia pneumoniae
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Chlamydia pneumoniae
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Chlamydia pneumoniae
dTMP + tetrahydrofolate + FAD
-
637235
Chlamydia pneumoniae
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Chlamydia psittaci
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Chlamydia psittaci
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Chlamydia psittaci
dTMP + tetrahydrofolate + FAD
-
637235
Chlamydia psittaci
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Helicobacter pylori
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Helicobacter pylori
dTMP + tetrahydrofolate + FAD
-
637235
Helicobacter pylori
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Thermotoga maritima
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Thermotoga maritima
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Thermotoga maritima
dTMP + tetrahydrofolate + FAD
-
637235
Thermotoga maritima
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Bacillus anthracis
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Bacillus anthracis
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Bacillus anthracis
dTMP + tetrahydrofolate + FAD
-
637235
Bacillus anthracis
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Rickettsia conorii
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Rickettsia conorii
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Rickettsia conorii
dTMP + tetrahydrofolate + FAD
-
637235
Rickettsia conorii
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Ehrlichia chaffeensis
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Ehrlichia chaffeensis
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Ehrlichia chaffeensis
dTMP + tetrahydrofolate + FAD
-
637235
Ehrlichia chaffeensis
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Thermotoga maritima DSM 3109
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Thermotoga maritima DSM 3109
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Thermotoga maritima DSM 3109
dTMP + tetrahydrofolate + FAD
-
637235
Thermotoga maritima DSM 3109
?
Subunits
Subunits
Commentary
Organism
tetramer
-
Helicobacter pylori
tetramer
data from crystal structure
Thermotoga maritima
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Bacillus anthracis
FAD
-
Borreliella burgdorferi
FAD
-
Campylobacter jejuni
FAD
-
Chlamydia pneumoniae
FAD
-
Chlamydia psittaci
FAD
-
Chlamydia trachomatis
FAD
-
Clostridioides difficile
FAD
-
Clostridium botulinum
FAD
-
Clostridium perfringens
FAD
-
Corynebacterium diphtheriae
FAD
tightly bound, four molecules per tetramer
Dictyostelium discoideum
FAD
-
Ehrlichia chaffeensis
FAD
-
Mycobacterium avium
FAD
-
Mycobacterium leprae
FAD
-
Mycobacterium tuberculosis variant bovis
FAD
-
Rhodococcus sp.
FAD
-
Rickettsia conorii
FAD
-
Rickettsia prowazekii
FAD
even though FAD is very well bound to three subunits, FAD interactions are not an absolute requirement for tetramer stabilization; tightly bound, four molecules per tetramer
Thermotoga maritima
FAD
-
Treponema denticola
FAD
-
Treponema pallidum
FAD
-
Mycobacterium tuberculosis
FAD
-
Helicobacter pylori
FADH2
-
Bacillus anthracis
FADH2
-
Borreliella burgdorferi
FADH2
-
Campylobacter jejuni
FADH2
-
Chlamydia pneumoniae
FADH2
-
Chlamydia psittaci
FADH2
-
Chlamydia trachomatis
FADH2
-
Clostridioides difficile
FADH2
-
Clostridium botulinum
FADH2
-
Clostridium perfringens
FADH2
-
Corynebacterium diphtheriae
FADH2
-
Ehrlichia chaffeensis
FADH2
-
Helicobacter pylori
FADH2
-
Mycobacterium avium
FADH2
-
Mycobacterium leprae
FADH2
-
Mycobacterium tuberculosis
FADH2
-
Mycobacterium tuberculosis variant bovis
FADH2
-
Rhodococcus sp.
FADH2
-
Rickettsia conorii
FADH2
-
Rickettsia prowazekii
FADH2
-
Thermotoga maritima
FADH2
-
Treponema denticola
FADH2
-
Treponema pallidum
NAD(P)H
required
Thermotoga maritima
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
NADH
required to reduce bound FAD
Dictyostelium discoideum
NADH
-
Helicobacter pylori
NADH
required to reduce bound FAD
Thermotoga maritima
NADPH
required to reduce bound FAD
Dictyostelium discoideum
NADPH
-
Helicobacter pylori
NADPH
required to reduce bound FAD
Thermotoga maritima
Application (protein specific)
Application
Commentary
Organism
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Bacillus anthracis
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Borreliella burgdorferi
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Campylobacter jejuni
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Chlamydia pneumoniae
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Chlamydia psittaci
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Chlamydia trachomatis
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low crossreactivity against mammalian thyA enzymes, EC 2.1.1.45
Clostridioides difficile
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low crossreactivity against mammalian thyA enzymes, EC 2.1.1.45
Clostridium botulinum
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Clostridium perfringens
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low crossreactivity against mammalian thyA enzymes, EC 2.1.1.45
Corynebacterium diphtheriae
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Ehrlichia chaffeensis
medicine
specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Helicobacter pylori
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human
Helicobacter pylori
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Mycobacterium avium
medicine
specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Mycobacterium leprae
medicine
specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Mycobacterium tuberculosis
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Mycobacterium tuberculosis variant bovis
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Rhodococcus sp.
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Rickettsia conorii
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Rickettsia prowazekii
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Treponema denticola
medicine
promising medical target, thyX is present in a number of pathogenic bacteria but absent in human; specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45
Treponema pallidum
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Thermotoga maritima
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Bacillus anthracis
FAD
-
Borreliella burgdorferi
FAD
-
Campylobacter jejuni
FAD
-
Chlamydia pneumoniae
FAD
-
Chlamydia psittaci
FAD
-
Chlamydia trachomatis
FAD
-
Clostridioides difficile
FAD
-
Clostridium botulinum
FAD
-
Clostridium perfringens
FAD
-
Corynebacterium diphtheriae
FAD
tightly bound, four molecules per tetramer
Dictyostelium discoideum
FAD
-
Ehrlichia chaffeensis
FAD
-
Mycobacterium avium
FAD
-
Mycobacterium leprae
FAD
-
Mycobacterium tuberculosis variant bovis
FAD
-
Rhodococcus sp.
FAD
-
Rickettsia conorii
FAD
-
Rickettsia prowazekii
FAD
even though FAD is very well bound to three subunits, FAD interactions are not an absolute requirement for tetramer stabilization; tightly bound, four molecules per tetramer
Thermotoga maritima
FAD
-
Treponema denticola
FAD
-
Treponema pallidum
FAD
-
Mycobacterium tuberculosis
FAD
-
Helicobacter pylori
FADH2
-
Bacillus anthracis
FADH2
-
Borreliella burgdorferi
FADH2
-
Campylobacter jejuni
FADH2
-
Chlamydia pneumoniae
FADH2
-
Chlamydia psittaci
FADH2
-
Chlamydia trachomatis
FADH2
-
Clostridioides difficile
FADH2
-
Clostridium botulinum
FADH2
-
Clostridium perfringens
FADH2
-
Corynebacterium diphtheriae
FADH2
-
Ehrlichia chaffeensis
FADH2
-
Helicobacter pylori
FADH2
-
Mycobacterium avium
FADH2
-
Mycobacterium leprae
FADH2
-
Mycobacterium tuberculosis
FADH2
-
Mycobacterium tuberculosis variant bovis
FADH2
-
Rhodococcus sp.
FADH2
-
Rickettsia conorii
FADH2
-
Rickettsia prowazekii
FADH2
-
Thermotoga maritima
FADH2
-
Treponema denticola
FADH2
-
Treponema pallidum
NAD(P)H
required
Thermotoga maritima
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Thermotoga maritima
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
FAD
-
Thermotoga maritima
HEPES
-
Thermotoga maritima
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5,10-methylenetetrahydrofolate + dUMP + FADH2
Chlamydia trachomatis
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Dictyostelium discoideum
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Mycobacterium avium
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Mycobacterium leprae
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Mycobacterium tuberculosis
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Rhodococcus sp.
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Clostridium perfringens
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Mycobacterium tuberculosis variant bovis
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Clostridium botulinum
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Clostridioides difficile
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Corynebacterium diphtheriae
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Treponema denticola
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Treponema pallidum
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Rickettsia prowazekii
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Borreliella burgdorferi
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Campylobacter jejuni
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Chlamydia pneumoniae
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Chlamydia psittaci
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Helicobacter pylori
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Thermotoga maritima
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Bacillus anthracis
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Rickettsia conorii
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Ehrlichia chaffeensis
-
dTMP + tetrahydrofolate + FAD
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
Thermotoga maritima DSM 3109
-
dTMP + tetrahydrofolate + FAD
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermotoga maritima
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Chlamydia trachomatis
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Chlamydia trachomatis
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Chlamydia trachomatis
dTMP + tetrahydrofolate + FAD
-
637235
Chlamydia trachomatis
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Dictyostelium discoideum
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Dictyostelium discoideum
dTMP + tetrahydrofolate + FAD
-
637235
Dictyostelium discoideum
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium avium
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium avium
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium avium
dTMP + tetrahydrofolate + FAD
-
637235
Mycobacterium avium
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium leprae
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium leprae
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium leprae
dTMP + tetrahydrofolate + FAD
-
637235
Mycobacterium leprae
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium tuberculosis
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium tuberculosis
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium tuberculosis
dTMP + tetrahydrofolate + FAD
-
637235
Mycobacterium tuberculosis
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Helicobacter pylori
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Rhodococcus sp.
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Rhodococcus sp.
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Rhodococcus sp.
dTMP + tetrahydrofolate + FAD
-
637235
Rhodococcus sp.
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Clostridium perfringens
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Clostridium perfringens
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Clostridium perfringens
dTMP + tetrahydrofolate + FAD
-
637235
Clostridium perfringens
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium tuberculosis variant bovis
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium tuberculosis variant bovis
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Mycobacterium tuberculosis variant bovis
dTMP + tetrahydrofolate + FAD
-
637235
Mycobacterium tuberculosis variant bovis
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Clostridium botulinum
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Clostridium botulinum
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Clostridium botulinum
dTMP + tetrahydrofolate + FAD
-
637235
Clostridium botulinum
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Clostridioides difficile
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Clostridioides difficile
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Clostridioides difficile
dTMP + tetrahydrofolate + FAD
-
637235
Clostridioides difficile
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Corynebacterium diphtheriae
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Corynebacterium diphtheriae
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Corynebacterium diphtheriae
dTMP + tetrahydrofolate + FAD
-
637235
Corynebacterium diphtheriae
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Treponema denticola
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Treponema denticola
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Treponema denticola
dTMP + tetrahydrofolate + FAD
-
637235
Treponema denticola
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Treponema pallidum
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Treponema pallidum
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Treponema pallidum
dTMP + tetrahydrofolate + FAD
-
637235
Treponema pallidum
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Rickettsia prowazekii
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Rickettsia prowazekii
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Rickettsia prowazekii
dTMP + tetrahydrofolate + FAD
-
637235
Rickettsia prowazekii
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Borreliella burgdorferi
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Borreliella burgdorferi
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Borreliella burgdorferi
dTMP + tetrahydrofolate + FAD
-
637235
Borreliella burgdorferi
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Campylobacter jejuni
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Campylobacter jejuni
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Campylobacter jejuni
dTMP + tetrahydrofolate + FAD
-
637235
Campylobacter jejuni
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Chlamydia pneumoniae
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Chlamydia pneumoniae
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Chlamydia pneumoniae
dTMP + tetrahydrofolate + FAD
-
637235
Chlamydia pneumoniae
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Chlamydia psittaci
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Chlamydia psittaci
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Chlamydia psittaci
dTMP + tetrahydrofolate + FAD
-
637235
Chlamydia psittaci
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Helicobacter pylori
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Helicobacter pylori
dTMP + tetrahydrofolate + FAD
-
637235
Helicobacter pylori
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Thermotoga maritima
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Thermotoga maritima
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Thermotoga maritima
dTMP + tetrahydrofolate + FAD
-
637235
Thermotoga maritima
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Bacillus anthracis
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Bacillus anthracis
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Bacillus anthracis
dTMP + tetrahydrofolate + FAD
-
637235
Bacillus anthracis
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Rickettsia conorii
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Rickettsia conorii
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Rickettsia conorii
dTMP + tetrahydrofolate + FAD
-
637235
Rickettsia conorii
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Ehrlichia chaffeensis
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Ehrlichia chaffeensis
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Ehrlichia chaffeensis
dTMP + tetrahydrofolate + FAD
-
637235
Ehrlichia chaffeensis
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Thermotoga maritima DSM 3109
dTMP + tetrahydrofolate + FAD
-
-
-
-
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Thermotoga maritima DSM 3109
dTMP + tetrahydrofolate + FAD
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
637235
Thermotoga maritima DSM 3109
dTMP + tetrahydrofolate + FAD
-
637235
Thermotoga maritima DSM 3109
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
-
Helicobacter pylori
tetramer
data from crystal structure
Thermotoga maritima
Other publictions for EC 2.1.1.148
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733512
Alexandrova
Synthesis and evaluation of C- ...
Mycobacterium tuberculosis
Bioorg. Med. Chem.
23
7131-7137
2015
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733298
Becker
Substrate interaction dynamics ...
Paramecium bursaria Chlorella virus 1
Biochem. J.
459
37-45
2014
-
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4
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1
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-
734029
Mishanina
Substrate activation in flavin ...
Thermotoga maritima, Thermotoga maritima ATCC 43589
J. Am. Chem. Soc.
136
10597-10600
2014
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5
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2
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-
-
-
-
734346
Mathews
Flavin-dependent thymidylate s ...
Thermotoga maritima, Thermotoga maritima ATCC 43589
J. Bioterror Biodef.
Suppl 12
004
2013
-
-
1
1
1
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5
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-
719663
Mishanina
Trapping of an intermediate in ...
Thermotoga maritima, Thermotoga maritima ATCC 43589
J. Am. Chem. Soc.
134
4442-4448
2012
-
-
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5
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1
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2
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1
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1
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1
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2
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-
720985
Zhang
Crystal structure of a flavin- ...
Helicobacter pylori
Protein Pept. Lett.
19
1225-1230
2012
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1
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6
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1
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1
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1
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-
720992
Wang
Crystal structure and enzymati ...
Helicobacter pylori, Helicobacter pylori SS1
Protein Sci.
20
1398-1410
2011
1
-
1
1
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1
2
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3
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4
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1
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1
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1
1
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1
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3
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1
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2
1
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1
1
-
-
-
-
-
-
-
-
718969
Kan
Biochemical characterization o ...
no activity in Corynebacterium glutamicum strain NCHU 87078
Biochim. Biophys. Acta
1804
1751-1759
2010
-
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1
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1
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-
-
-
-
-
-
697906
Wang
Oxidase activity of a flavin-d ...
Thermotoga maritima
FEBS J.
276
2801-2810
2009
-
-
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-
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1
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2
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700395
Koehn
An unusual mechanism of thymid ...
Thermotoga maritima
Nature
458
919-923
2009
-
-
-
1
2
-
-
-
-
-
-
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2
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1
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1
2
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-
691285
Esra Onen
Design, synthesis and evaluati ...
Paramecium bursaria Chlorella virus 1
Bioorg. Med. Chem. Lett.
18
3628-3631
2008
-
-
-
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-
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5
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1
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1
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1
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2
5
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2
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5
5
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1
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1
-
-
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-
-
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-
-
-
-
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-
-
694812
Hunter
Kinetics and ligand-binding pr ...
Mycobacterium tuberculosis
PLoS ONE
3
e2237
2008
-
1
1
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1
3
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1
2
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1
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1
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3
1
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2
1
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1
1
2
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1
1
3
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1
2
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1
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3
1
-
-
-
-
-
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-
-
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-
698579
Ulmer
Functional analysis of the Myc ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Bacteriol.
190
2056-2064
2008
-
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-
-
1
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-
-
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10
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1
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-
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-
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-
-
-
-
-
700990
Escartin
Flavin-dependent thymidylate s ...
Helicobacter pylori, Paramecium bursaria Chlorella virus 1
Proc. Natl. Acad. Sci. USA
105
9948-9952
2008
-
-
-
-
-
-
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4
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-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
671555
Chernyshev
Thymidyl biosynthesis enzymes ...
Bacillus anthracis, Chlamydia trachomatis, Clostridium botulinum, Helicobacter pylori, Mycobacterium leprae, Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, no activity in Homo sapiens, Paramecium bursaria Chlorella virus-1, Pyrococcus furiosus, Rickettsia sp., Thermotoga maritima, Treponema palladium
Appl. Microbiol. Biotechnol.
74
282-289
2007
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1
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3
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2
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17
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3
1
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2
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1
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2
3
-
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2
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3
1
-
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-
-
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-
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691759
Chernyshev
The relationships between oxid ...
Thermotoga maritima
Chem. Commun. (Camb. )
27
2861-2863
2007
-
1
-
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-
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1
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1
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1
-
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692837
Leduc
Flavin-dependent thymidylate s ...
Rhodobacter capsulatus, Rhodobacter capsulatus MT1131
J. Bacteriol.
189
8537-8545
2007
-
-
-
-
-
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2
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6
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4
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2
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2
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2
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4
-
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678023
Kanai
Archaeal Pyrococcus furiosus t ...
Pyrococcus furiosus
Biochem. J.
393
373-379
2006
-
1
1
-
-
-
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1
1
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3
-
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1
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1
1
1
1
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1
1
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1
1
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1
-
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1
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
679184
Mason
A lag-phase in the reduction o ...
Thermotoga maritima
Chem. Commun. (Camb. )
16
1781-1783
2006
-
-
-
-
-
-
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1
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-
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1
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1
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1
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1
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1
-
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-
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-
-
-
-
680675
Graziani
Catalytic mechanism and struct ...
Paramecium bursaria Chlorella virus-1
J. Biol. Chem.
281
24048-24057
2006
-
-
1
1
9
-
2
-
-
-
-
-
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4
-
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1
-
-
-
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1
1
1
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2
-
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2
-
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1
2
1
9
-
2
2
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1
-
-
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1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681384
Sampathkumar
NADP+ expels both the co-facto ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Biol.
360
1-6
2006
-
1
1
1
1
-
-
-
-
-
-
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7
-
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1
-
-
-
-
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1
1
-
-
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-
-
-
-
2
-
-
-
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1
1
2
1
1
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-
-
-
-
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1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658992
Gattis
Direct observation of the part ...
Campylobacter jejuni
J. Am. Chem. Soc.
127
832-833
2005
-
-
-
-
-
-
-
-
-
-
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1
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-
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-
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1
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2
-
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2
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-
-
1
-
-
-
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-
-
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-
-
-
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-
-
-
-
681375
Sampathkumar
Structure of the Mycobacterium ...
Mycobacterium tuberculosis
J. Mol. Biol.
352
1091-1104
2005
-
1
1
1
11
-
-
-
-
-
-
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7
-
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1
-
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-
-
1
1
1
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
1
11
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
1
1
-
-
-
-
-
-
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-
657899
Leduc
Two distinct pathways for thym ...
Aeropyrum pernix, Aquifex aeolicus, no activity in Archaeoglobus fulgidus, no activity in Methanocaldococcus jannaschii, no activity in Methanopyrus kandleri, no activity in Methanopyrus kandleri AV19, no activity in Methanothermobacter thermoautotrophicus, Pyrobaculum aerophilum, Pyrococcus abyssi, Pyrococcus furiosus, Pyrococcus horikoshii, Saccharolobus solfataricus, Sulfurisphaera tokodaii, Thermoplasma acidophilum, Thermoplasma volcanium, Thermotoga maritima
Biochem. Soc. Trans.
32
231-235
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
17
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
22
-
-
-
-
-
-
22
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658015
Agrawal
Mechanistic studies of a flavi ...
Thermotoga maritima, Thermotoga maritima DSM 3109
Biochemistry
43
10295-10301
2004
-
1
-
-
-
-
1
-
-
-
-
1
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659052
Liu
Bacterial thymidylate synthase ...
Trichodesmium erythraeum
J. Bacteriol.
186
6316-6319
2004
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
1
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-
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-
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-
-
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-
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-
-
1
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659429
Graziani
Functional analysis of FAD-dep ...
Paramecium bursaria Chlorella virus-1
J. Biol. Chem.
279
54340-54347
2004
-
-
1
-
1
-
5
2
-
-
1
-
-
5
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
-
1
-
-
5
-
2
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
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-
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-
660374
Leduc
Functional evidence for active ...
Helicobacter pylori
Proc. Natl. Acad. Sci. USA
101
7252-7257
2004
-
-
-
-
10
-
1
7
-
-
-
-
-
4
-
-
1
-
-
-
-
-
1
1
-
-
-
7
-
-
-
1
-
-
-
-
-
-
1
-
10
-
-
1
-
7
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
7
-
-
-
-
-
-
-
-
-
-
637234
Montfort
Complementing thymidylate synt ...
Dictyostelium discoideum, Thermotoga maritima
Structure
11
607-608
2003
4
-
-
-
-
-
-
-
-
-
-
2
-
2
-
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-
-
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-
4
-
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-
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-
-
-
2
-
-
-
4
-
-
2
-
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-
-
-
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-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637235
Mathews
Functional analysis of substra ...
Bacillus anthracis, Borreliella burgdorferi, Campylobacter jejuni, Chlamydia pneumoniae, Chlamydia psittaci, Chlamydia trachomatis, Clostridioides difficile, Clostridium botulinum, Clostridium perfringens, Corynebacterium diphtheriae, Dictyostelium discoideum, Ehrlichia chaffeensis, Helicobacter pylori, Mycobacterium avium, Mycobacterium leprae, Mycobacterium tuberculosis, Mycobacterium tuberculosis variant bovis, Rhodococcus sp., Rickettsia conorii, Rickettsia prowazekii, Thermotoga maritima, Thermotoga maritima DSM 3109, Treponema denticola, Treponema pallidum
Structure
11
677-690
2003
6
21
1
1
-
-
2
-
-
-
-
24
-
26
-
-
1
-
-
-
-
-
71
2
-
-
-
-
-
-
-
46
-
-
-
6
22
1
46
1
-
-
-
2
-
-
-
-
-
24
-
-
-
1
-
-
-
-
71
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637232
Myllykallio
An alternative flavin-dependen ...
Borreliella burgdorferi, Campylobacter jejuni, Chlamydia sp., Dictyostelium discoideum, Helicobacter pylori, Mycobacterium tuberculosis, Pyrococcus abyssi, Pyrococcus horikoshii, Rickettsia prowazekii, Saccharolobus solfataricus, Treponema pallidum
Science
297
105-107
2002
3
4
2
-
1
-
-
-
-
-
3
11
-
11
-
-
1
-
-
-
-
-
33
1
-
-
-
-
-
-
-
22
-
-
-
3
4
2
22
-
1
-
-
-
-
-
-
-
3
11
-
-
-
1
-
-
-
-
33
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637233
Giladi
Genetic evidence for a novel t ...
Campylobacter jejuni, Halobacterium salinarum
FEMS Microbiol. Lett.
216
105-109
2002
-
-
2
-
-
-
1
-
-
-
1
2
-
2
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
1
2
-
-
-
1
-
-
-
-
4
-
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-