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2-ketohexanoic acid
pentanal + CO2
-
1% activity compared to phenylpyruvate
-
-
?
2-oxo-4-phenylbutanoic acid
?
9% activity compared to phenylpyruvate
-
-
?
2-Oxo-gamma-ethylthiobutanoate
beta-Ethiopropanal + CO2
2-Oxo-isohexanoate
?
-
at approximately 5% of the activity with phenylpyruvate
-
-
?
2-oxobutanoic acid
?
0.2% activity compared to phenylpyruvate
-
-
?
2-Oxohexanoate
Pentanal + CO2
2-oxohexanoic acid
?
6% activity compared to phenylpyruvate
-
-
?
2-oxopentanoic acid
?
1% activity compared to phenylpyruvate
-
-
?
3-methyl-2-oxobutanoic acid
?
1% activity compared to phenylpyruvate
-
-
?
3-methyl-2-oxopentanoic acid
?
2% activity compared to phenylpyruvate
-
-
?
4-hydroxyphenylpyruvate
4-hydroxyphenylacetaldehyde + CO2
63% activity compared to phenylpyruvate
-
-
?
4-methyl-2-oxopentanoic acid
?
6% activity compared to phenylpyruvate
-
-
?
4-methylthio-2-oxobutanoate
beta-methiopropanal + CO2
4-methylthio-2-oxobutanoic acid
?
6% activity compared to phenylpyruvate
-
-
?
4-phenyl-2-oxobutanoic acid
3-phenylpropanal + CO2
-
2.3% activity compared to phenylpyruvate
-
-
?
5-phenyl-2-oxopentanoic acid
4-phenylbutanal + CO2
-
110% activity compared to phenylpyruvate
-
-
?
benzoylformic acid
?
0.2% activity compared to phenylpyruvate
-
-
?
indole-3-pyruvate
indoleacetaldehyde + CO2
indole-3-pyruvic acid + acetaldehyde
3-hydroxy-1-(3-indoyl)-2-butanone
Achromobacter eurydice
-
-
-
?
indolepyruvate
indoleacetaldehyde + CO2
Indolepyruvate
Indoleethanal + CO2
L-phenylalanine
phenylacetic acid + CO2
-
-
-
-
?
L-tryptophan
indole-acetic acid + CO2
-
-
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
phenylpyruvate + acetaldehyde
3-hydroxy-1-phenyl-2-butanone
phenylpyruvate + n-butyraldehyde
3-hydroxy-1-phenyl-2-hexanone
phenylpyruvate + pentanal
3-hydroxy-1-phenyl-2-heptanone
phenylpyruvate + propionaldehyde
3-hydroxy-1-phenyl-2-pentanone
pyruvic acid
?
0.02% activity compared to phenylpyruvate
-
-
?
additional information
?
-
2-Oxo-gamma-ethylthiobutanoate
beta-Ethiopropanal + CO2
-
-
-
-
?
2-Oxo-gamma-ethylthiobutanoate
beta-Ethiopropanal + CO2
-
at approximately 30% of the activity with phenylpyruvate
-
-
?
2-Oxohexanoate
Pentanal + CO2
-
-
-
-
?
2-Oxohexanoate
Pentanal + CO2
-
at approximately 31% of the activity with phenylpyruvate
-
-
?
4-methylthio-2-oxobutanoate
beta-methiopropanal + CO2
-
-
-
-
?
4-methylthio-2-oxobutanoate
beta-methiopropanal + CO2
-
at approximately 23% of the activity with phenylpyruvate
-
-
?
indole-3-pyruvate
indoleacetaldehyde + CO2
-
10.4% activity compared to phenylpyruvate
-
-
?
indole-3-pyruvate
indoleacetaldehyde + CO2
preferred substrate, 100% activity
-
-
?
indolepyruvate
indoleacetaldehyde + CO2
-
-
-
-
?
indolepyruvate
indoleacetaldehyde + CO2
-
higher affinity for indolepyruvate than for phenylpyruvate but a 10fold higher specificity for the latter
-
-
?
indolepyruvate
indoleacetaldehyde + CO2
-
-
-
-
?
Indolepyruvate
Indoleethanal + CO2
-
-
-
-
?
Indolepyruvate
Indoleethanal + CO2
-
at 48% of the activity with phenylpyruvate
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
-
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
100% activity
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
converts phenylpyruvate with a 10fold higher specificity than indolepyruvate despite a slightly higher affinity for the latter
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
-
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
-
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
-
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
-
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
-
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
-
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
first step in the Ehrlich pathway
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
preferred substrate, 100% activity
-
-
?
Phenylpyruvate
Phenylacetaldehyde + CO2
-
-
-
?
phenylpyruvate + acetaldehyde
3-hydroxy-1-phenyl-2-butanone
Achromobacter eurydice
-
stereoselective
-
?
phenylpyruvate + acetaldehyde
3-hydroxy-1-phenyl-2-butanone
Achromobacter eurydice
-
stereoselective, best yields of 34-35% is obtained at acetaldehyde concentrations of 50-100 mM with 5 mM concentration of phenylpyruvate using the crude cell extract
-
?
phenylpyruvate + acetaldehyde
3-hydroxy-1-phenyl-2-butanone
Achromobacter eurydice SC 16386
-
stereoselective
-
?
phenylpyruvate + acetaldehyde
3-hydroxy-1-phenyl-2-butanone
Achromobacter eurydice SC 16386
-
stereoselective, best yields of 34-35% is obtained at acetaldehyde concentrations of 50-100 mM with 5 mM concentration of phenylpyruvate using the crude cell extract
-
?
phenylpyruvate + acetaldehyde
3-hydroxy-1-phenyl-2-butanone
-
stereoselective
-
?
phenylpyruvate + acetaldehyde
3-hydroxy-1-phenyl-2-butanone
-
stereoselective
-
?
phenylpyruvate + acetaldehyde
3-hydroxy-1-phenyl-2-butanone
-
-
-
?
phenylpyruvate + n-butyraldehyde
3-hydroxy-1-phenyl-2-hexanone
Achromobacter eurydice
-
-
-
?
phenylpyruvate + n-butyraldehyde
3-hydroxy-1-phenyl-2-hexanone
Achromobacter eurydice SC 16386
-
-
-
?
phenylpyruvate + pentanal
3-hydroxy-1-phenyl-2-heptanone
Achromobacter eurydice
-
-
-
?
phenylpyruvate + pentanal
3-hydroxy-1-phenyl-2-heptanone
Achromobacter eurydice SC 16386
-
-
-
?
phenylpyruvate + propionaldehyde
3-hydroxy-1-phenyl-2-pentanone
Achromobacter eurydice
-
-
-
?
phenylpyruvate + propionaldehyde
3-hydroxy-1-phenyl-2-pentanone
Achromobacter eurydice SC 16386
-
-
-
?
additional information
?
-
Achromobacter eurydice
-
the acyloic condensation yield decreases with increasing chain length for straigth chain aliphatic aldehydes from 76% for acetaldehyde to 42% for valeraldehyde, using longer chain aldehydes, aromatic aldehydes, bromoacetaldehyde, bromopropionaldehyde and acrolin, either none or negligible amounts of the acyloin products are obtained
-
?
additional information
?
-
Achromobacter eurydice SC 16386
-
the acyloic condensation yield decreases with increasing chain length for straigth chain aliphatic aldehydes from 76% for acetaldehyde to 42% for valeraldehyde, using longer chain aldehydes, aromatic aldehydes, bromoacetaldehyde, bromopropionaldehyde and acrolin, either none or negligible amounts of the acyloin products are obtained
-
?
additional information
?
-
-
strictly inducible enzyme
-
-
?
additional information
?
-
-
completely inactive toward benzoylformate
-
-
?
additional information
?
-
-
no activity with benzoylformate and indole-glyoxylic acid
-
-
?
additional information
?
-
-
enzyme is involved in Phe catabolism
-
-
?
additional information
?
-
-
enzyme is involved in Phe catabolism
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
double mutants blocked in both phenylalanine dehydrogenase and phenylpyruvate decarboxylase completely fail to catabolize Phe
-
-
?
additional information
?
-
-
enzyme is involved in Phe catabolism
-
-
?
additional information
?
-
-
double mutants blocked in both phenylalanine dehydrogenase and phenylpyruvate decarboxylase completely fail to catabolize Phe
-
-
?
additional information
?
-
-
enzyme is involved in Phe catabolism
-
-
?
additional information
?
-
PPDC is essentially unable to utilize pyruvate and 3-indoleglyoxylic acid
-
-
?
additional information
?
-
-
PPDC is essentially unable to utilize pyruvate and 3-indoleglyoxylic acid
-
-
?
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5.51
2-ketohexanoic acid
-
in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C
0.09
2-oxo-4-phenylbutanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
5.5 - 7.6
2-oxobutanoic acid
0.17 - 1
2-oxohexanoic acid
0.37 - 2.1
2-oxopentanoic acid
8.5
3-methyl-2-oxobutanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
3.1
3-methyl-2-oxopentanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.09
4-hydroxyphenylpyruvate
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.9
4-methyl-2-oxopentanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.64
4-methylthio-2-oxobutanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
1.91
4-phenyl-2-oxobutanoic acid
-
in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C
0.3
5-phenyl-2-oxopentanoic acid
-
in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C
0.03 - 0.13
Indole-3-pyruvate
0.0051 - 1.13
phenylpyruvate
5.5
2-oxobutanoic acid
mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
6.4
2-oxobutanoic acid
mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
7
2-oxobutanoic acid
mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
7.6
2-oxobutanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.17
2-oxohexanoic acid
mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.46
2-oxohexanoic acid
mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.55
2-oxohexanoic acid
mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.69
2-oxohexanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
1
2-oxohexanoic acid
mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.37
2-oxopentanoic acid
mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.54
2-oxopentanoic acid
mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.63
2-oxopentanoic acid
mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.96
2-oxopentanoic acid
mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
2.1
2-oxopentanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.03
Indole-3-pyruvate
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.13
Indole-3-pyruvate
-
in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C
0.0051
phenylpyruvate
-
-
0.054
phenylpyruvate
mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.062
phenylpyruvate
-
strain CEN.PK 608-4B
0.1
phenylpyruvate
-
strain CEN.PK 113-7D
0.1
phenylpyruvate
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.18
phenylpyruvate
mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.48
phenylpyruvate
-
strain CEN.PK 555-4A
0.8
phenylpyruvate
mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
1.08
phenylpyruvate
-
in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C
1.13
phenylpyruvate
allosteric enzyme, Hill coefficient 4.7, pH 7.5, temperature not specified in the publication
5.5
Pyruvic acid
mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
6.4
Pyruvic acid
mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
9.7
Pyruvic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
25
Pyruvic acid
mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.29
2-ketohexanoic acid
-
in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C
1.7
2-oxo-4-phenylbutanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.04 - 3.9
2-oxobutanoic acid
0.41 - 8.8
2-oxohexanoic acid
0.39 - 5.2
2-oxopentanoic acid
19
3-methyl-2-oxobutanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
11
3-methyl-2-oxopentanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
11
4-hydroxyphenylpyruvate
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
10
4-methyl-2-oxopentanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
7.7
4-methylthio-2-oxobutanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.21
4-phenyl-2-oxobutanoic acid
-
in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C
1.7
5-phenyl-2-oxopentanoic acid
-
in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C
0.07 - 5.4
Indole-3-pyruvate
0.0055 - 20
phenylpyruvate
0.064 - 0.45
Pyruvic acid
additional information
phenylpyruvate
-
in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C
0.04
2-oxobutanoic acid
mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.06
2-oxobutanoic acid
mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
2.2
2-oxobutanoic acid
mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
3.9
2-oxobutanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.41
2-oxohexanoic acid
mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.96
2-oxohexanoic acid
mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
1.3
2-oxohexanoic acid
mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
3.3
2-oxohexanoic acid
mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
8.8
2-oxohexanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.39
2-oxopentanoic acid
mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.76
2-oxopentanoic acid
mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
2
2-oxopentanoic acid
mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
2.1
2-oxopentanoic acid
mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
5.2
2-oxopentanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.07
Indole-3-pyruvate
-
in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C
0.14
Indole-3-pyruvate
mutant enzyme E545L, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
5.4
Indole-3-pyruvate
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.0055
phenylpyruvate
allosteric enzyme, Hill coefficient 4.7, pH 7.5, temperature not specified in the publication
0.85
phenylpyruvate
mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
1.2
phenylpyruvate
mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
4.8
phenylpyruvate
mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
5.56
phenylpyruvate
-
in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C
8.8
phenylpyruvate
mutant enzyme E545L, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
20
phenylpyruvate
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.064
Pyruvic acid
mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.32
Pyruvic acid
mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.34
Pyruvic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.45
Pyruvic acid
mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
19
2-oxo-4-phenylbutanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.007 - 0.52
2-oxobutanoic acid
0.42 - 13
2-oxohexanoic acid
0.4 - 3.7
2-oxopentanoic acid
2.2
3-methyl-2-oxobutanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
3.5
3-methyl-2-oxopentanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
125
4-hydroxyphenylpyruvate
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
11
4-methyl-2-oxopentanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
12
4-methylthio-2-oxobutanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.35
benzoylformic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
200
Indole-3-pyruvate
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.0058 - 200
phenylpyruvate
0.003 - 0.07
Pyruvic acid
0.007
2-oxobutanoic acid
mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.009
2-oxobutanoic acid
mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.315
2-oxobutanoic acid
mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.52
2-oxobutanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.42
2-oxohexanoic acid
mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
2.8
2-oxohexanoic acid
mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
5.8
2-oxohexanoic acid
mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
6
2-oxohexanoic acid
mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
13
2-oxohexanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.4
2-oxopentanoic acid
mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
2.1
2-oxopentanoic acid
mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
2.5
2-oxopentanoic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
3.4
2-oxopentanoic acid
mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
3.7
2-oxopentanoic acid
mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.0058
phenylpyruvate
allosteric enzyme, Hill coefficient 4.7, pH 7.5, temperature not specified in the publication
0.03
phenylpyruvate
mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
1.1
phenylpyruvate
mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
200
phenylpyruvate
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.003
Pyruvic acid
mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.027
Pyruvic acid
mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.035
Pyruvic acid
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.06
Pyruvic acid
mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
0.07
Pyruvic acid
mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C
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De Boer, L.; Harder, W.; Dijkhuizen, L.
Phenylalanine and tyrosine metabolism in the facultative methylotroph Nocardia sp. 239
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1988
Nocardia sp., Nocardia sp. 239
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Brevibacterium linens, Brevibacterium linens 47
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Barrowman, M.M.; Fewson, C.A.
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Acinetobacter calcoaceticus
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Kishore, G.; Sugumaran, M.; Vaidyanathan, C.S.
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Aspergillus niger
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Meyerozyma guilliermondii
brenda
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Methods Enzymol.
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585-596
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Acinetobacter sp.
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Enzymatic conversion of phenylpyruvate to phenylacetate
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170
375-391
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Acinetobacter sp.
brenda
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69
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Saccharomyces cerevisiae
brenda
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Asymmetric acyloin condensation catalyzed by phenylpyruvate decarboxylase
Tetrahedron
10
4667-4675
1999
Achromobacter eurydice, Pseudomonas putida, Pseudomonas chlororaphis subsp. aurantiaca, Thauera aromatica, Achromobacter eurydice SC 16386, Thauera aromatica SC 16387, Pseudomonas chlororaphis subsp. aurantiaca SC 16387
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brenda
Guo, Z.; Goswami, A.; Nanduri, V.B.; Patel, R.N.
Asymmetric acyloin condensation catalysed by phenylpyruvate decarboxylase. Part 2: Substrate specificity and purification of the enzyme
Tetrahedron
12
571-577
2001
Achromobacter eurydice, Achromobacter eurydice SC 16386
-
brenda
Versees, W.; Spaepen, S.; Vanderleyden, J.; Steyaert, J.
The crystal structure of phenylpyruvate decarboxylase from Azospirillum brasilense at 1.5 A resolution. Implications for its catalytic and regulatory mechanism
FEBS J.
274
2363-2375
2007
Azospirillum brasilense
brenda
Spaepen, S.; Versees, W.; Gocke, D.; Pohl, M.; Steyaert, J.; Vanderleyden, J.
Characterization of phenylpyruvate decarboxylase, involved in auxin production of Azospirillum brasilense
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189
7626-7633
2007
Azospirillum brasilense
brenda
Versees, W.; Spaepen, S.; Wood, M.D.; Leeper, F.J.; Vanderleyden, J.; Steyaert, J.
Molecular mechanism of allosteric substrate activation in a thiamine diphosphate-dependent decarboxylase
J. Biol. Chem.
282
35269-35278
2007
Azospirillum brasilense
brenda
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Simultaneous synthesis of 2-phenylethanol and L-homophenylalanine using aromatic transaminase with yeast Ehrlich pathway
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102
1323-1329
2008
Saccharomyces cerevisiae
brenda
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Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae
FEBS J.
278
1842-1853
2011
Saccharomyces cerevisiae (Q06408), Saccharomyces cerevisiae
brenda
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Indole-3-acetic acid biosynthesis in the biocontrol strain Pseudomonas fluorescens Psd and plant growth regulation by hormone overexpression
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162
426-435
2011
Azospirillum brasilense, no activity in Pseudomonas fluorescens, no activity in Pseudomonas fluorescens Psd, Azospirillum brasilense SM
brenda
Kang, Z.; Zhang, C.; Du, G.; Chen, J.
Metabolic engineering of Escherichia coli for production of 2-phenylethanol from renewable glucose
Appl. Biochem. Biotechnol.
172
2012-2021
2014
Komagataella pastoris, Komagataella pastoris GS115
brenda
Veiga, T.; Solis-Escalante, D.; Romagnoli, G.; ten Pierick, A.; Hanemaaijer, M.; Deshmukh, A.T.; Deshmuhk, A.; Wahl, A.; Pronk, J.T.; Daran, J.M.
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11
238-249
2012
Penicillium chrysogenum
brenda
de Souza Liberal, A.T.; Carazzolle, M.F.; Pereira, G.A.; Simoes, D.A.; de Morais, M.A.
The yeast Dekkera bruxellensis genome contains two orthologs of the ARO10 gene encoding for phenylpyruvate decarboxylase
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28
2473-2478
2012
Brettanomyces bruxellensis, Brettanomyces bruxellensis GDB 248
brenda
Xu, X.; Wang, C.; Chen, J.; Yang, S.
Streptomyces virginiae PPDC is a new type of phenylpyruvate decarboxylase composed of two subunits
ACS Chem. Biol.
12
2008-2014
2017
Streptomyces virginiae (A0A222AKA3), Streptomyces virginiae
brenda
Kim, T.; Lee, S.; Oh, M.
Biosynthesis of 2-phenylethanol from glucose with genetically engineered Kluyveromyces marxianus
Enzyme Microb. Technol.
61-62
44-47
2014
Saccharomyces cerevisiae, Saccharomyces cerevisiae YPH499
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brenda
Masuo, S.; Osada, L.; Zhou, S.; Fujita, T.; Takaya, N.
Aspergillus oryzae pathways that convert phenylalanine into the flavor volatile 2-phenylethanol
Fungal Genet. Biol.
77
22-30
2015
Aspergillus oryzae (Q2UKV4), Aspergillus oryzae
brenda