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Literature summary for 4.1.1.43 extracted from

  • Versees, W.; Spaepen, S.; Vanderleyden, J.; Steyaert, J.
    The crystal structure of phenylpyruvate decarboxylase from Azospirillum brasilense at 1.5 A resolution. Implications for its catalytic and regulatory mechanism (2007), FEBS J., 274, 2363-2375.
    View publication on PubMed
Search for more literature for 4.1.1.43

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Bl21-CodonPlus (DE3)-RP cells Azospirillum brasilense

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method with precipitant solution containing 15% PEG4000 (w /v), 10% glycerol (v /v) in 100 mM HEPES buffer pH 7.5, at 20°C Azospirillum brasilense

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, PPDC binds two molecules of Mg2+ per dimer Azospirillum brasilense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 4 * 58000, X-ray crystallography, asymmetric dimer of dimers, in the asymmetric unit two subunits of PPDC are tightly packed to form a dimer related by noncrystallographic two-fold symmetry Azospirillum brasilense

Organism

Organism UniProt Comment Textmining
Azospirillum brasilense
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-nitrilotriacetic acid affinity chromatography and Superdex-200 gel filtration Azospirillum brasilense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
indolepyruvate higher affinity for indolepyruvate than for phenylpyruvate but a 10fold higher specificity for the latter Azospirillum brasilense indoleacetaldehyde + CO2
-
 ?
additional information completely inactive toward benzoylformate Azospirillum brasilense ?
-
 ?
Phenylpyruvate converts phenylpyruvate with a 10fold higher specificity than indolepyruvate despite a slightly higher affinity for the latter Azospirillum brasilense Phenylacetaldehyde + CO2
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 58000, X-ray crystallography, asymmetric dimer of dimers, in the asymmetric unit two subunits of PPDC are tightly packed to form a dimer related by noncrystallographic two-fold symmetry Azospirillum brasilense

Synonyms

Synonyms Comment Organism
phenyl/indolepyruvate decarboxylase
-
 Azospirillum brasilense
PPDC
-
 Azospirillum brasilense

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate dependent, PPDC binds two molecules of thiamine diphosphate per dimer Azospirillum brasilense