EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    4.1.1.43 | -999 |
- |
phenylpyruvate |
in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C |
680508 |
| 4.1.1.43 | 0.0055 |
- |
phenylpyruvate |
allosteric enzyme, Hill coefficient 4.7, pH 7.5, temperature not specified in the publication |
746595 |
| 4.1.1.43 | 0.04 |
- |
2-oxobutanoic acid |
mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
| 4.1.1.43 | 0.06 |
- |
2-oxobutanoic acid |
mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
| 4.1.1.43 | 0.064 |
- |
Pyruvic acid |
mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
| 4.1.1.43 | 0.07 |
- |
Indole-3-pyruvate |
in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C |
680508 |
| 4.1.1.43 | 0.14 |
- |
Indole-3-pyruvate |
mutant enzyme E545L, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
| 4.1.1.43 | 0.21 |
- |
4-phenyl-2-oxobutanoic acid |
in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C |
680508 |
| 4.1.1.43 | 0.29 |
- |
2-ketohexanoic acid |
in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C |
680508 |
| 4.1.1.43 | 0.32 |
- |
Pyruvic acid |
mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
