Information on EC 2.6.1.29 - diamine transaminase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

top print hide
EC NUMBER
COMMENTARY hide
2.6.1.29
-
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RECOMMENDED NAME
GeneOntology No.
diamine transaminase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an alpha,omega-diamine + 2-oxoglutarate = an omega-aminoaldehyde + L-glutamate
show the reaction diagram
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
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-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Arginine and proline metabolism
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-
Metabolic pathways
-
-
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SYSTEMATIC NAME
IUBMB Comments
diamine:2-oxoglutarate aminotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9031-83-8
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top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-1,4-diaminobutan-2-ol + 2-oxoglutarate
?
show the reaction diagram
-
30% of the activity with putrescine
-
-
-
1,6-diaminohexane + 2-oxoglutarate
6-aminohexanal + L-glutamate
show the reaction diagram
-
-
-
?
1,7-diaminoheptane + 2-oxoglutarate
7-aminoheptanal + L-glutamate
show the reaction diagram
4-aminobutanoate + 2-oxoglutarate
4-oxobutanoate + L-glutamate
show the reaction diagram
cadaverine + 2-oxoglutarate
5-aminopentanal + L-glutamate
show the reaction diagram
putrescine + 2-oxoglutarate
4-aminobutanal + L-glutamate
show the reaction diagram
putrescine + pyruvate
4-aminobutanal + L-alanine
show the reaction diagram
additional information
?
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cadaverine + 2-oxoglutarate
5-aminopentanal + L-glutamate
show the reaction diagram
putrescine + 2-oxoglutarate
4-aminobutanal + L-glutamate
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cyanide
-
complete inhibition at 0.5 mM
hydroxylamine
-
complete inhibition at 0.5 mM
Semicarbazide
-
complete inhibition at 0.5 mM
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.88
2-oxoglutarate
-
pH 7.6, room temperature
2.7
pyruvate
-
pH 7.6, room temperature
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00064
additional information
-
values with different nitrogen sources
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 10.5
-
almost completely inactive at pH 7, pH 8 about 40% of maximum activity, pH 10.5 about 20% of maximum activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15ºC, 0.03 M Tris-HCl buffer, pH 7.6, 1 month
-
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
precipitation with ammonium sulphate followed by column chromatography
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LINKS TO OTHER DATABASES (specific for EC-Number 2.6.1.29)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)