BRENDA - Enzyme Database
show all sequences of 2.6.1.29

Purification and properties of a diamine alpha-ketoglutarate transaminase from Escherichia coli

Kim, K.H.; J. Biol. Chem. 239, 783-786 (1964)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.88
-
2-oxoglutarate
pH 7.6, room temperature
Escherichia coli
2.7
-
pyruvate
pH 7.6, room temperature
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
putrescine + 2-oxoglutarate
Escherichia coli
-
4-aminobutanal + L-glutamate
-
Escherichia coli
?
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
precipitation with ammonium sulphate followed by column chromatography
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.63
-
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1,7-diaminoheptane + 2-oxoglutarate
30% of the activity with putrescine
639950
Escherichia coli
7-aminoheptanal + L-glutamate
-
639950
Escherichia coli
?
4-aminobutanoate + 2-oxoglutarate
11% of the activity with putrescine
639950
Escherichia coli
4-oxobutanoate + L-glutamate
-
639950
Escherichia coli
?
cadaverine + 2-oxoglutarate
equally active as putrescine
639950
Escherichia coli
5-aminopentanal + L-glutamate
-
639950
Escherichia coli
?
additional information
inactive with oxaloacetate
639950
Escherichia coli
?
-
-
-
?
additional information
inactive with 1,3-diaminopropane, lysine, ornithine, spermidine
639950
Escherichia coli
?
-
-
-
?
putrescine + 2-oxoglutarate
-
639950
Escherichia coli
4-aminobutanal + L-glutamate
-
639950
Escherichia coli
?
putrescine + pyruvate
-
639950
Escherichia coli
4-aminobutanal + L-alanine
-
639950
Escherichia coli
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
10
-
Escherichia coli
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8
10.5
almost completely inactive at pH 7, pH 8 about 40% of maximum activity, pH 10.5 about 20% of maximum activity
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
required
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
required
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.88
-
2-oxoglutarate
pH 7.6, room temperature
Escherichia coli
2.7
-
pyruvate
pH 7.6, room temperature
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
putrescine + 2-oxoglutarate
Escherichia coli
-
4-aminobutanal + L-glutamate
-
Escherichia coli
?
Purification (Commentary) (protein specific)
Commentary
Organism
precipitation with ammonium sulphate followed by column chromatography
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.63
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1,7-diaminoheptane + 2-oxoglutarate
30% of the activity with putrescine
639950
Escherichia coli
7-aminoheptanal + L-glutamate
-
639950
Escherichia coli
?
4-aminobutanoate + 2-oxoglutarate
11% of the activity with putrescine
639950
Escherichia coli
4-oxobutanoate + L-glutamate
-
639950
Escherichia coli
?
cadaverine + 2-oxoglutarate
equally active as putrescine
639950
Escherichia coli
5-aminopentanal + L-glutamate
-
639950
Escherichia coli
?
additional information
inactive with oxaloacetate
639950
Escherichia coli
?
-
-
-
?
additional information
inactive with 1,3-diaminopropane, lysine, ornithine, spermidine
639950
Escherichia coli
?
-
-
-
?
putrescine + 2-oxoglutarate
-
639950
Escherichia coli
4-aminobutanal + L-glutamate
-
639950
Escherichia coli
?
putrescine + pyruvate
-
639950
Escherichia coli
4-aminobutanal + L-alanine
-
639950
Escherichia coli
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
10
-
Escherichia coli
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8
10.5
almost completely inactive at pH 7, pH 8 about 40% of maximum activity, pH 10.5 about 20% of maximum activity
Escherichia coli
Other publictions for EC 2.6.1.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
639952
Bascarán
Stringent response and initiat ...
Streptomyces clavuligerus
J. Gen. Microbiol.
137
1625-1634
1991
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1
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1
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1
-
-
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-
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639953
Madduri
Lysine catabolism in Streptomy ...
Streptomyces phaeochromogenes, Amycolatopsis lactamdurans, Streptomyces clavuligerus, Streptomyces glaucescens, Streptomyces griseus, Streptomyces lividans, Streptomyces parvulus, Streptomyces rimosus, Streptomyces venezuelae, Streptomyces viridochromogenes, Streptomyces glaucescens GLAO, Streptomyces viridochromogenes CUB416, Streptomyces phaeochromogenes B2196, Streptomyces parvulus ISP5048, Streptomyces lividans TK24, Streptomyces clavuligerus NRRL 3585, Streptomyces griseus NRRL 3851, Streptomyces venezuelae ISP5230, Amycolatopsis lactamdurans NRRL 3802, Streptomyces rimosus NRRL 2234
J. Bacteriol.
171
299-302
1989
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20
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27
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10
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40
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10
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20
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10
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40
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10
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639951
Kim
-
Diamine-alpha-ketoglutarate am ...
Escherichia coli
Methods Enzymol.
17B
812-815
1971
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3
2
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1
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1
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1
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1
1
9
-
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1
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1
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1
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3
-
2
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1
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1
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1
1
9
-
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1
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639950
Kim
Purification and properties of ...
Escherichia coli
J. Biol. Chem.
239
783-786
1964
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2
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1
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1
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1
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1
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7
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1
1
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1
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1
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2
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1
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1
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1
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7
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1
1
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