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Literature summary for 2.6.1.29 extracted from
- Purification and properties of a diamine alpha-ketoglutarate transaminase from Escherichia coli (1964), J. Biol. Chem., 239, 783-786.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.88 | - |
2-oxoglutarate | pH 7.6, room temperature | Escherichia coli |  |
| 2.7 | - |
pyruvate | pH 7.6, room temperature | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| putrescine + 2-oxoglutarate | Escherichia coli | - |
4-aminobutanal + L-glutamate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| precipitation with ammonium sulphate followed by column chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.63 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,7-diaminoheptane + 2-oxoglutarate | 30% of the activity with putrescine | Escherichia coli | 7-aminoheptanal + L-glutamate | - |
? | |
| 4-aminobutanoate + 2-oxoglutarate | 11% of the activity with putrescine | Escherichia coli | 4-oxobutanoate + L-glutamate | - |
? | |
| cadaverine + 2-oxoglutarate | equally active as putrescine | Escherichia coli | 5-aminopentanal + L-glutamate | - |
? | |
| additional information | inactive with oxaloacetate | Escherichia coli | ? | - |
? | |
| additional information | inactive with 1,3-diaminopropane, lysine, ornithine, spermidine | Escherichia coli | ? | - |
? | |
| putrescine + 2-oxoglutarate | - |
Escherichia coli | 4-aminobutanal + L-glutamate | - |
? | |
| putrescine + pyruvate | - |
Escherichia coli | 4-aminobutanal + L-alanine | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 10 | - |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10.5 | almost completely inactive at pH 7, pH 8 about 40% of maximum activity, pH 10.5 about 20% of maximum activity | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | required | Escherichia coli | |
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Release 2023.1 (January 2023)
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