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6.3.4.14: biotin carboxylase

This is an abbreviated version!
For detailed information about biotin carboxylase, go to the full flat file.

Word Map on EC 6.3.4.14

Reaction

ATP
+
[biotin carboxyl-carrier protein]-biotin-N6-L-lysine
+
hydrogencarbonate-
=
ADP
+
phosphate
+
[biotin carboxyl-carrier protein]-carboxybiotin-N6-L-lysine

Synonyms

ACC, AccA, AccBC, AccC, BC, biotin carboxylase, biotin carboxylase (component of acetyl CoA carboxylase), biotinoyl domain of acetyl-CoA carboxylase, BirA, Carboxylase, biotin, More, PC-beta

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.4 Other carbon-nitrogen ligases
                6.3.4.14 biotin carboxylase

Cloned

Cloned on EC 6.3.4.14 - biotin carboxylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
accA gene, sequencing, expression in Escherichia coli, forms together with accB a two-gene operon
accC gene, overexpression in Escherichia coli
-
expressed in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)pLysS
-
expression of biotin carboxylase domain of acetyl-coenzyme A carboxylase in Escherichia coli
expression of biotin carboxylase domain of pyruvate carboxylase in Escherichia coli
-
expression of the His-tagged biotinoyl domain in Escherichia coli strain AVB101
-
expression of wild-type and mutant His-tagged biotinoyl domains in Escherichia coli strain AVB101
-
gene accC, DNA and amino acid sequence determination, analysis, and comparison, phylogenetic analysis, and denaturing gradient gel electrophoresis genetic fingerprinting method development and evaluation, overview
gene accC, expression in Escherichia coli srrain BL21-AI as His-tagged enzyme
-
gene accC, expression in Escherichia coli strain BL21-AI as His-tagged enzyme
-
gene accC, overexpression in Escherichia coli strain BL21(DE3) as His-tagged enzyme
-
gene encoding a protein composed of two domains, an N-terminal biotin carboxylase and a C-terminal biotin-carboxyl-carrier protein
-
overexpression in Escherichia coli JM109
overexpression system
-
truncated biotin carboxylase domain of acetyl-CoA carboxylase, expression in Escherichia coli