6.3.4.14: biotin carboxylase
This is an abbreviated version!
For detailed information about biotin carboxylase, go to the full flat file.
Word Map on EC 6.3.4.14
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6.3.4.14
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carboxyltransferase
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carboxylases
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biotin-dependent
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malonyl-coa
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acetyl-coenzyme
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propionyl-coa
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carboxybiotin
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6.4.1.2
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biotin-carboxyl
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biotin-containing
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bicarbonate-dependent
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biotin-binding
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carboxyphosphate
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transcarboxylase
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mgatp-dependent
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accases
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atp-grasp
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medicine
- 6.3.4.14
- carboxyltransferase
- carboxylases
-
biotin-dependent
- malonyl-coa
-
acetyl-coenzyme
- propionyl-coa
- carboxybiotin
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6.4.1.2
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biotin-carboxyl
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biotin-containing
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bicarbonate-dependent
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biotin-binding
- carboxyphosphate
- transcarboxylase
-
mgatp-dependent
- accases
-
atp-grasp
- medicine
Reaction
Synonyms
ACC, AccA, AccBC, AccC, BC, biotin carboxylase, biotin carboxylase (component of acetyl CoA carboxylase), biotinoyl domain of acetyl-CoA carboxylase, BirA, Carboxylase, biotin, More, PC-beta
ECTree
6.3.4.14 biotin carboxylaseAdvanced search results
results (
results (KM Value
KM Value on EC 6.3.4.14 - biotin carboxylase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.16
biotin-carboxyl-carrier protein
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C-terminal 87 amino acids of the biotinylated biotin-carboxyl-carrier protein
62.2
CO2
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recombinant biotin carboxylase domain of pyruvate carboxylase
0.08
ADP
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pH 8, 25°C, wild-type enzyme, ATP synthesis reaction with carbamoyl phosphate as cosubstrate
0.18
ADP
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pH 8, 25°C, E211A mutant, ATP synthesis reaction with carbamoyl phosphate as cosubstrate
0.19
ADP
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pH 8, 25°C, wild-type enzyme, ATP synthesis reaction with carbamoyl phosphate as cosubstrate
0.23
ADP
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pH 8, 25°C, E288A mutant, ATP synthesis reaction with carbamoyl phosphate as cosubstrate
0.4
ADP
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pH 8, 25°C, N290A mutant, ATP synthesis reaction with carbamoyl phosphate as cosubstrate
0.83
ADP
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pH 8, 25°C, R292A mutant, ATP synthesis reaction with carbamoyl phosphate as cosubstrate
0.081
ATP
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25°C, wild-type enzyme, bicarbonate-dependent ATP hydrolysis in the absence of biotin
0.1 - 2
ATP
mutant enzyme R16E, in 100 mM HEPES (pH 8.0), 5 mM MgCl2, at 30°C
0.1 - 2
ATP
wild type enzyme, in 100 mM HEPES (pH 8.0), 5 mM MgCl2, at 30°C
4.23
ATP
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25°C, C230A mutant, bicarbonate-dependent ATP hydrolysis in the absence of biotin
6.87
ATP
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25°C, K238Q mutant, bicarbonate-dependent ATP hydrolysis in the absence of biotin
50.9
biotin
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recombinant biotin carboxylase domain of pyruvate carboxylase
0.51
Carbamoyl phosphate
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pH 8, 25°C, E211A mutant, ATP synthesis reaction with ADP as cosubstrate
2.3
Carbamoyl phosphate
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pH 8, 25°C, E288A mutant, ATP synthesis reaction with ADP as cosubstrate
2.8
Carbamoyl phosphate
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pH 8, 25°C, R292A mutant, ATP synthesis reaction with ADP as cosubstrate
4.8
Carbamoyl phosphate
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pH 8, 25°C, wild-type enzyme, ATP synthesis reaction with ADP as cosubstrate
11.2
Carbamoyl phosphate
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pH 8, 25°C, wild-type enzyme, ATP synthesis reaction with ADP as cosubstrate
0.37
HCO3-
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25°C, wild-type enzyme, bicarbonate-dependent ATP hydrolysis in the absence of biotin
0.7
HCO3-
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25°C, C230A mutant, bicarbonate-dependent ATP hydrolysis in the absence of biotin
1.9
HCO3-
wild type enzyme, with 1.5 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C
5
HCO3-
wild type enzyme, with 7.5 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C
6.5
HCO3-
wild type enzyme, with 10 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C
11.4
HCO3-
wild type enzyme, with 20 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C
