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6.2.1.25: benzoate-CoA ligase

This is an abbreviated version!
For detailed information about benzoate-CoA ligase, go to the full flat file.

Word Map on EC 6.2.1.25

Reaction

ATP
+
benzoate
 +
CoA
=
AMP
 +
diphosphate
 +
benzoyl-CoA

Synonyms

AMP-forming BCL, AMP-forming benzoate-CoA ligase, BadA, BamY, Bcl, bclA, BCLM, benzoate CoA ligase, Benzoate coenzyme A ligase, benzoate-CoA ligase, Benzoate-CoA ligase (AMP-forming), Benzoyl coenzyme A synthetase, Benzoyl-CoA ligase, Benzoyl-CoA synthetase, BzdA enzyme, Synthetase, benzoyl coenzyme A, XabE

ECTree

     6 Ligases
         6.2 Forming carbon-sulfur bonds
             6.2.1 Acid-thiol ligases
                6.2.1.25 benzoate-CoA ligase

Specific Activity

Specific Activity on EC 6.2.1.25 - benzoate-CoA ligase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.07
-
pH 7.5, 22°C
0.081
soluble protein, for benzoyl-CoA synthesis, in 100 mM Tris-HCl (pH 7.8), 10mM KCl, 2.5mM MgCl2, 10mM phospho(enol)-pyruvate, 0.5 mM ATP, 0.25 mM CoA, 0.2mM NADH, 2 units myokinase, 2 units pyruvate kinase, 2U of lactate dehydrogenase, and 0.1 mM substrate
13.4
after 166fold purification, for benzoyl-CoA synthesis, in 100 mM Tris-HCl (pH 7.8), 10 mM KCl, 2.5 mM MgCl2, 10 mM phospho(enol)-pyruvate, 0.5 mM ATP, 0.25 mM CoA, 0.2 mM NADH, 2 units myokinase, 2 units pyruvate kinase, 2 units of lactate dehydrogenase, and 0.1 mM substrate
16.5
-
37°C
additional information