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6.2.1.25: benzoate-CoA ligase

This is an abbreviated version!
For detailed information about benzoate-CoA ligase, go to the full flat file.

Word Map on EC 6.2.1.25

Reaction

ATP
+
benzoate
+
CoA
=
AMP
+
diphosphate
+
benzoyl-CoA

Synonyms

AMP-forming BCL, AMP-forming benzoate-CoA ligase, BadA, BamY, Bcl, bclA, BCLM, benzoate CoA ligase, Benzoate coenzyme A ligase, benzoate-CoA ligase, Benzoate-CoA ligase (AMP-forming), Benzoyl coenzyme A synthetase, Benzoyl-CoA ligase, Benzoyl-CoA synthetase, BzdA enzyme, Synthetase, benzoyl coenzyme A, XabE

ECTree

     6 Ligases
         6.2 Forming carbon-sulfur bonds
             6.2.1 Acid-thiol ligases
                6.2.1.25 benzoate-CoA ligase

Purification

Purification on EC 6.2.1.25 - benzoate-CoA ligase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
at 4°C, by a method that includes DEAE-sepharose, source 30Q and reactive green chromatography
-
DEAE, anion-exchange, HAP, affinity on reactive red resin and anion-exchange on a Mono-Q chromatography
-
DEAE-Toyopearl column chromatography, Phenyl-Sepharose Fast Flow column chromatography, and Superose 12 gel filtration
His-tagged recombinant enzyme
-
Ni-NTA resin column chromatography
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration