Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoCl2 | - |
Anaerobic bacterium | |
CuCl2 | CuCl2 | Anaerobic bacterium | |
EDTA | - |
Anaerobic bacterium | |
FeCl2 | - |
Anaerobic bacterium | |
NiCl2 | - |
Anaerobic bacterium | |
Zn2+ | ZnCl2 | Anaerobic bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
benzoate | - |
Anaerobic bacterium | |
0.06 | - |
CoASH | - |
Anaerobic bacterium | |
0.56 | - |
ATP | - |
Anaerobic bacterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Anaerobic bacterium | |
Mg2+ | optimal concentration: 5 mM MgCl2 | Anaerobic bacterium | |
Mn2+ | can replace Mg2+ | Anaerobic bacterium | |
Mn2+ | optimal concentration: 5 mM MnCl2, 85% of the efficiency compared to MgCl2 | Anaerobic bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
7 or 8 * 58000, SDS-PAGE | Anaerobic bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anaerobic bacterium | - |
a strictly anaerobic, obligate synthrophic mixed culture | - |
Purification (Comment) | Organism |
---|---|
- |
Anaerobic bacterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Anaerobic bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-fluorobenzoate + CoA | 93% of the activity relative to benzoate | Anaerobic bacterium | AMP + diphosphate + 2-fluorobenzoyl-CoA | - |
? | |
ATP + 3-fluorobenzoate + CoA | 56% of the activity relative to benzoate | Anaerobic bacterium | AMP + diphosphate + 3-fluorobenzoyl-CoA | - |
? | |
ATP + 4-fluorobenzoate + CoA | 75% of the activity relative to benzoate | Anaerobic bacterium | AMP + diphosphate + 4-fluorobenzoyl-CoA | - |
? | |
ATP + benzoate + CoA | - |
Anaerobic bacterium | AMP + diphosphate + benzoyl-CoA | - |
? | |
ATP + isonicotinate + CoA | 29% of the activity relative to benzoate | Anaerobic bacterium | AMP + diphosphate + isonicotinyl-CoA | - |
? | |
ATP + nicotinate + CoA | 11% of the activity relative to benzoate | Anaerobic bacterium | AMP + diphosphate + nicotinyl-CoA | - |
? | |
ATP + picolinate + CoA | 46% of the activity relative to benzoate | Anaerobic bacterium | AMP + diphosphate + picolinyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 7 or 8 * 58000, SDS-PAGE | Anaerobic bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Anaerobic bacterium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 45 | 22°C: about 40% of maximal activity, 45°C: about 20% of maximal activity | Anaerobic bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Anaerobic bacterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | 7: about 40% of maximal activity, 9: about 30% of maximal activity | Anaerobic bacterium |