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Literature summary for 6.2.1.25 extracted from

  • Geissler, J.F.; Harwood, C.S.; Gibson, J.
    Purification and properties of benzoate-coenzyme A ligase, a Rhodopseudomonas palustris enzyme involved in the anaeriobic degradation of benzoate (1988), J. Bacteriol., 170, 1709-1714.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.25

Inhibitors

Inhibitors Comment Organism Structure
benzoyl-CoA product inhibition Rhodopseudomonas palustris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006 0.002 benzoate
-
 Rhodopseudomonas palustris
0.002 0.003 ATP
-
 Rhodopseudomonas palustris
0.09 0.12 reduced CoA
-
 Rhodopseudomonas palustris

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Rhodopseudomonas palustris
Mn2+ can replace Mg2+ Rhodopseudomonas palustris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 HPLC gel filtration Rhodopseudomonas palustris
60000
-
 1 * 60000, SDS-PAGE Rhodopseudomonas palustris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + benzoate + CoA Rhodopseudomonas palustris enzyme is involved in anaerobic degradation of benzoate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodopseudomonas palustris
-
-
-

Oxidation Stability

Oxidation Stability Organism
purified enzyme is insensitive to oxygen Rhodopseudomonas palustris

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodopseudomonas palustris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25
-
-
 Rhodopseudomonas palustris

Storage Stability

Storage Stability Organism
0°C, 1 mg/ml purified enzyme, half-life is 8 days Rhodopseudomonas palustris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-chlorobenzoate + CoA 10% of the activity relative to benzoate Rhodopseudomonas palustris AMP + diphosphate + 2-chlorobenzoyl-CoA
-
 ?
ATP + 2-fluorobenzoate + CoA 102% of the activity relative to benzoate Rhodopseudomonas palustris AMP + diphosphate + 2-fluorobenzoyl-CoA
-
 ?
ATP + 4-fluorobenzoate + CoA 72% of the activity relative to benzoate Rhodopseudomonas palustris AMP + diphosphate + 4-fluorobenzoyl-CoA
-
 ?
ATP + benzoate + CoA
-
 Rhodopseudomonas palustris AMP + diphosphate + benzoyl-CoA
-
 ?
ATP + benzoate + CoA enzyme is involved in anaerobic degradation of benzoate Rhodopseudomonas palustris ?
-
 ?
ATP + DELTA1-cyclohexenecarboxylate + CoA 13% of the activity relative to benzoate Rhodopseudomonas palustris AMP + diphosphate + DELTA1-cyclohexenecarboxyl-CoA
-
 ?
ATP + DELTA3-cyclohexenecarboxylate + CoA 54% of the activity relative to benzoate Rhodopseudomonas palustris AMP + diphosphate + DELTA3-cyclohexenecarboxyl-CoA
-
 ?
ATP + nicotinate + CoA 12% of the activity relative to benzoate Rhodopseudomonas palustris AMP + diphosphate + nicotinyl-CoA
-
 ?
ATP + picolinate + CoA 45% of the activity relative to benzoate Rhodopseudomonas palustris AMP + diphosphate + picolinyl-CoA
-
 ?
additional information no reaction with GTP Rhodopseudomonas palustris ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE Rhodopseudomonas palustris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4 8.9
-
 Rhodopseudomonas palustris

pH Range

pH Minimum pH Maximum Comment Organism
7 8.9 7.0: 10% of maximal activity, 8.0: 75% of maximal activity, 8.4-8.9: maximal activity Rhodopseudomonas palustris