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4.3.2.1: argininosuccinate lyase

This is an abbreviated version!
For detailed information about argininosuccinate lyase, go to the full flat file.

Word Map on EC 4.3.2.1

Reaction

2-(Nomega-L-arginino)succinate
=
fumarate
 +
L-arginine

Synonyms

AL, ARG4, ArgH, arginine-succinate lyase, argininosuccinase, Argininosuccinate lyase, argininosuccinic acid lyase, Arginosuccinase, ASAL, ASL, delta2 crystallin, delta2-crystallin, hASL, L-argininosuccinate arginine-lyase, lyase, argininosuccinate, Rv1659

ECTree

     4 Lyases
         4.3 Carbon-nitrogen lyases
             4.3.2 Amidine-lyases
                4.3.2.1 argininosuccinate lyase

Temperature Stability

Temperature Stability on EC 4.3.2.1 - argininosuccinate lyase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38
-
30 min, stable
4
-
Tris-HCl buffer, pH 8.5, 50% loss of activity after 2.3 h
42.4
Tm-value: mutant enzyme V335L
42.5
Tm-value of mutant enzyme C441A
46.5
Tm-value: mutant enzyme R95C
47
Tm-value of wild-type enzyme
48
Tm-value: mutant enzyme R379C
48.1
Tm-value: mutant enzyme E189G
48.5
Tm-value: mutant enzyme I100T
49
-
20 min, 50% inactivation
49.9
Tm-value: mutant enzyme V178M
52
-
20 min, 50% inactivation
52.7
Tm-value: wild-type enzyme
53
Tm-value of mutant enzyme C441S
54
-
mid-point temperature transition 2
57
-
mid-point temperature transition 1 and 3
58
-
mid-point temperature transition 3, presence of alphaA-crystallin
65
-
complete denaturation
83
-
mid-point temperature transition 1, presence of alphaA-crystallin
85
-
mid-point temperature transition 2, presence of alphaA-crystallin
additional information
-
enzyme forms with higher isoelectric point are much more thermostable than those with lower pI values