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Literature summary for 4.3.2.1 extracted from

  • Mishra, A.; Surolia, A.
    Biochemical characterization of argininosuccinate lyase from M. tuberculosis significance of a c-terminal cysteine in catalysis and thermal stability (2017), IUBMB Life, 69, 896-907 .
    View publication on PubMed
Search for more literature for 4.3.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21-DE3 Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
C441A striking decrease in the enzymatic activity, while retaining the overall secondary to quaternary structure of the protein Mycobacterium tuberculosis
C441S significant increase in activity, as compared to the wild-type enzyme, the mutant enzyme has higher thermal stability and maintains significant activity at high temperatures. The mutant shows a marked increase in enzymatic activity as it lacks this cysteine dependent feedback inhibition Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
fumarate a potential feedback mechanism is proposed, wherein the Cys441 is covalently modified to S-(2-succinyl) cysteine by one of the products, fumarate Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 2-(Nomega-L-arginino)succinate pH 7.4, 25°C, mutant enzyme C441S Mycobacterium tuberculosis
0.112
-
 2-(Nomega-L-arginino)succinate pH 7.4, 25°C, wild-type enzyme Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 non-denaturing PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-(Nomega-L-arginino)succinate Mycobacterium tuberculosis last enzyme of the arginine biosynthesis pathway fumarate + L-arginine
-
 ?
2-(Nomega-L-arginino)succinate Mycobacterium tuberculosis ATCC 25618 last enzyme of the arginine biosynthesis pathway fumarate + L-arginine
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WPY7
-
-
Mycobacterium tuberculosis ATCC 25618 P9WPY7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(Nomega-L-arginino)succinate
-
 Mycobacterium tuberculosis fumarate + L-arginine
-
 ?
2-(Nomega-L-arginino)succinate last enzyme of the arginine biosynthesis pathway Mycobacterium tuberculosis fumarate + L-arginine
-
 ?
2-(Nomega-L-arginino)succinate
-
 Mycobacterium tuberculosis ATCC 25618 fumarate + L-arginine
-
 ?
2-(Nomega-L-arginino)succinate last enzyme of the arginine biosynthesis pathway Mycobacterium tuberculosis ATCC 25618 fumarate + L-arginine
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 50900 Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
ArgH
-
 Mycobacterium tuberculosis
Rv1659
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42.5
-
 Tm-value of mutant enzyme C441A Mycobacterium tuberculosis
47
-
 Tm-value of wild-type enzyme Mycobacterium tuberculosis
53
-
 Tm-value of mutant enzyme C441S Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14.63
-
 2-(Nomega-L-arginino)succinate pH 7.4, 25°C, wild-type enzyme Mycobacterium tuberculosis
29.27
-
 2-(Nomega-L-arginino)succinate pH 7.4, 25°C, mutant enzyme C441S Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mycobacterium tuberculosis

General Information

General Information Comment Organism
metabolism last enzyme of the arginine biosynthesis pathway. Esential enzyme for the growth and survival of Mycobacterium tuberculosis Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
130
-
 2-(Nomega-L-arginino)succinate pH 7.4, 25°C, wild-type enzyme Mycobacterium tuberculosis
914
-
 2-(Nomega-L-arginino)succinate pH 7.4, 25°C, mutant enzyme C441S Mycobacterium tuberculosis