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4.3.2.1: argininosuccinate lyase

This is an abbreviated version!
For detailed information about argininosuccinate lyase, go to the full flat file.

Word Map on EC 4.3.2.1

Reaction

2-(Nomega-L-arginino)succinate
=
fumarate
 +
L-arginine

Synonyms

AL, ARG4, ArgH, arginine-succinate lyase, argininosuccinase, Argininosuccinate lyase, argininosuccinic acid lyase, Arginosuccinase, ASAL, ASL, delta2 crystallin, delta2-crystallin, hASL, L-argininosuccinate arginine-lyase, lyase, argininosuccinate, Rv1659

ECTree

     4 Lyases
         4.3 Carbon-nitrogen lyases
             4.3.2 Amidine-lyases
                4.3.2.1 argininosuccinate lyase

Cloned

Cloned on EC 4.3.2.1 - argininosuccinate lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of argH gene encoding ASL
-
cloning of cDNA
-
expressed as His tag enzyme
-
expressed as His tag enzymes
-
expression in Escherichia coli
expression in Escherichia coli and COS-7 cells
-
expression in Escherichia coli argH auxotroph, deficient in argininosuccinate lyase
-
expression in Escherichia coli BL21-DE3
expression in mouse cells
-
expression in Saccharomyces cerevisiae and in Escherichia coli
-
expression of ASL constructs in human embryonic kidney 293T cells
gene argH, expression of Rv1659 as His-tagged protein, with an extra methionine at the N-terminus and an extended sequence LEHHHHHH at the C-terminus, in Escherichia coli strain BL21 (DE3)
gene asl, quantitative realtime PCR expression analysis in infected and control cells
-
gene OsASL1, two variants OsASL1.1 and OsASL1.2 resulting from different transcription start sites, map-based cloning, only OsASL1.1 is able to complement the ref1 mutant phenotype, functional expression of OsASL1.1 in Saccharomyces cerevisiae, plastid-localized expression of OsASL1.1-GFP fusion protein in onion epidermal cells
-
in vitro overexpression of ASL enzyme mutant R236W in COS7 cells andprimary fibroblasts
-
into the pGEM-T vector for sequence analysis
overexpressed in HEK293T cells or in Escherichia coli
-