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4.1.3.36: 1,4-dihydroxy-2-naphthoyl-CoA synthase

This is an abbreviated version!
For detailed information about 1,4-dihydroxy-2-naphthoyl-CoA synthase, go to the full flat file.

Word Map on EC 4.1.3.36

Reaction

4-(2-carboxyphenyl)-4-oxobutanoyl-CoA
=
1,4-dihydroxy-2-naphthoyl-CoA
 +
H2O

Synonyms

1,4-dihydroxy-2-naphthoate synthetase, 1,4-dihydroxy-2-naphthoyl coenzyme A synthase, 1,4-dihydroxy-2-naphthoyl-CoA synthase, 1,4-dihydroxy-2-naphthoyl-coenzyme synthase, DHNA synthetase, DHNA-CoA synthase, dihydroxynaphthoate synthase, Dihydroxynaphthoic acid synthetase, MenB, naphthoate synthase, naphthoate synthase (MenB), Rv0548c, synthase, 1,4-dihydroxy-2-naphthoate

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.3 Oxo-acid-lyases
                4.1.3.36 1,4-dihydroxy-2-naphthoyl-CoA synthase

Crystallization

Crystallization on EC 4.1.3.36 - 1,4-dihydroxy-2-naphthoyl-CoA synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant enzyme free or in complex with bicarbonate or nitrate, hanging drop vapor diffusion method, 0.01 ml of protein solution containing 10 mg/mL purified ecMenB, 25 mM Tris, pH 8.0, and 10% glycerol, including 10 mM NaHCO3 or NaNO3 for the complexed enzyme, is mixed with 0.001 ml of reservoir solution containing reservoir solution containing 300 mM NaCl, 100 mM Tris, pH 7.5, 2% Tacsimate, and 20% PEG 335, equilibration against 0.5 ml of reservoir solution, 22°C, X-ray diffraction structure determination and analysis at 2.30 A resolution
-
purified recombinant enzyme, hanging drop method, mixing of protein solution containing 10 mg/ml protein in 10 mM NaHCO3, 10 mM 1-hydroxy-2-naphthoyl-CoA, and 25 mM Tris, pH 8.0, with reservoir solution containing 200 mM (NH4)2SO4 and 23% PEG 3,350 in 100 mM Bis-Tris, pH 5.5, in a 1:1 ratio, 1 week, X-ray diffraction structure determination and analysis at 1.84 A resolution
-
sitting drop vapor diffusion technique, PEG 3350, in complex with o-succinylbenzoyl-amino-CoA
-
sitting drop vapour diffusion method with 30% (v/v) pentaerythritol ethocylate, 0.05 M ammonium sulfate, and 0.05 M bis-Tris pH 6.5
-
hanging drop vapor diffusion technique, PEG 6000, in complex with o-succinylbenzoyl-aminoCoA
in complex with 1-hydroxy-2-naphthoyl-CoA or salicylyl-CoA, hanging drop vapor diffusion method, using 0.3 M NaCl, 0.1 M Tris-HCl pH 8.0, 25% (w/v) PEG 3350
sitting-drop or hanging-drop vapour-diffusion method, structure of native enzyme determined at 2.15 A resolution and structure of the naphthoyl-CoA complex is determined at 2.3 A resolution
sitting drop vapour diffusion method with 0.1 M NaHEPES pH 7.5, 1.6 M ammonium sulfate, 0.2 M NaCl
purified recombinant enzyme, hanging drop method, mixing of protein solution containing 10 mg/ml protein in 10 mM NaHCO3, 5 mM 1-hydroxy-2-naphthoyl-CoA, 5 mM salicyloyl-CoA, 20 mM glycine, pH 9.75, 1% glycerol, and 10 mM NaHCO3 or 0.15 M ammonium acetate, 4% tacsimate, 15% PEG 3350, and 100 mM Bis-Tris, pH 6.0, with reservoir solution containing 0.15 M ammonium acetate, 0.3 M ammonium sulfate, 16% PEG 3350, 100 mM Bis-Tris, pH 5.7, and 10 mM proline or 10 mM taurine, in a 1:1 ratio, 1 week, X-ray diffraction structure determination and analysis at 2.0-2.35 A resolution
purified recombinant enzyme, hanging drop vapor diffusion method, 0.01 ml of protein solution containing 10 mg/mL purified scMenB, 20 mM glycine, pH 9.75, and 1% glycerol, with or without 10 mM NaHCO3, is mixed with 0.001 ml of reservoir solution containing of 0.15 M ammonium acetate, 0.02 M L-proline, 0.1 M Bis-Tris, pH 6.1, and 45% MPD, equilibration against 0.5 ml of reservoir solution, 22°C, X-ray diffraction structure determination and analysis at 2.04 A resolution