Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Mycobacterium tuberculosis |
His-tagged version expressed in Escherichia coli BL21(DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion technique, PEG 6000, in complex with o-succinylbenzoyl-aminoCoA | Mycobacterium tuberculosis |
sitting drop vapor diffusion technique, PEG 3350, in complex with o-succinylbenzoyl-amino-CoA | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D185E | reduced kcat | Mycobacterium tuberculosis |
D185G | no activity | Mycobacterium tuberculosis |
D185N | reduced kcat | Mycobacterium tuberculosis |
D192N | no activity | Mycobacterium tuberculosis |
G156D | no activity | Escherichia coli |
S190A | reduced kcat | Mycobacterium tuberculosis |
Y287F | no activity | Mycobacterium tuberculosis |
Y97F | no activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
2-succinylbenzoyl-CoA | D185N mutant protein, pH 7.0, 25°C | Mycobacterium tuberculosis | |
0.0048 | - |
2-succinylbenzoyl-CoA | D185E mutant protein, pH 7.0, 25°C | Mycobacterium tuberculosis | |
0.0224 | - |
2-succinylbenzoyl-CoA | wild type protein, pH 7.0, 25°C | Mycobacterium tuberculosis | |
0.0259 | - |
o-succinylbenzoyl-CoA | pH 7.0, 25°C | Escherichia coli | |
0.0402 | - |
2-succinylbenzoyl-CoA | S190A mutant protein, pH 7.0, 25°C | Mycobacterium tuberculosis | |
0.106 | - |
2-(3-carboxypropyl)-benzoyl-CoA | wild type protein protein, pH 7.0, 25°C | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
6 * 30000, SDS-PAGE, crystallization | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Mycobacterium tuberculosis | P9WNP5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
His-tag affinity chromatography, tag removed | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-(3-carboxypropyl)-benzoyl-CoA | - |
Mycobacterium tuberculosis | ? | - |
? | |
2-succinylbenzoyl-CoA | - |
Mycobacterium tuberculosis | 1,4-dihydroxy-2-naphthoyl-CoA + H2O | - |
? | |
o-succinylbenzoyl-CoA | - |
Escherichia coli | 1,4-dihydroxy-2-naphthoyl-CoA + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | crystallization | Mycobacterium tuberculosis |
hexamer | 6 * 30000, SDS-PAGE, crystallization | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
1,4-dihydroxy-2-naphthoyl-CoA synthase | - |
Escherichia coli |
1,4-dihydroxy-2-naphthoyl-CoA synthase | - |
Mycobacterium tuberculosis |
MenB | - |
Escherichia coli |
MenB | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00022 | - |
2-succinylbenzoyl-CoA | D185N mutant protein, pH 7.0, 25°C | Mycobacterium tuberculosis | |
0.00025 | - |
2-(3-carboxypropyl)-benzoyl-CoA | wild type protein protein, pH 7.0, 25°C | Mycobacterium tuberculosis | |
0.0017 | - |
2-succinylbenzoyl-CoA | S190A mutant protein, pH 7.0, 25°C | Mycobacterium tuberculosis | |
0.0023 | - |
2-succinylbenzoyl-CoA | D185E mutant protein, pH 7.0, 25°C | Mycobacterium tuberculosis | |
0.062 | - |
o-succinylbenzoyl-CoA | pH 7.0, 25°C | Escherichia coli | |
0.462 | - |
2-succinylbenzoyl-CoA | wild type protein, pH 7.0, 25°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
2-(3-carboxypropyl)-benzoyl-CoA | wild type protein protein, pH 7.0, 25°C | Mycobacterium tuberculosis | |
0.04 | - |
2-succinylbenzoyl-CoA | S190A mutant protein, pH 7.0, 25°C | Mycobacterium tuberculosis | |
0.07 | - |
2-succinylbenzoyl-CoA | D185N mutant protein, pH 7.0, 25°C | Mycobacterium tuberculosis | |
0.48 | - |
2-succinylbenzoyl-CoA | D185E mutant protein, pH 7.0, 25°C | Mycobacterium tuberculosis | |
2.4 | - |
o-succinylbenzoyl-CoA | pH 7.0, 25°C | Escherichia coli | |
21 | - |
2-succinylbenzoyl-CoA | wild type protein, pH 7.0, 25°C | Mycobacterium tuberculosis |