BRENDA - Enzyme Database show
show all sequences of 4.1.3.36

Mechanism of the intramolecular Claisen condensation reaction catalyzed by MenB, a crotonase superfamily member

Li, H.J.; Li, X.; Liu, N.; Zhang, H.; Truglio, J.J.; Mishra, S.; Kisker, C.F.; Garcia-Diaz, M.; Tonge, P.J.; Biochemistry 50, 9532-9544 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3)
Mycobacterium tuberculosis
His-tagged version expressed in Escherichia coli BL21(DE3)
Escherichia coli
Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion technique, PEG 6000, in complex with o-succinylbenzoyl-aminoCoA
Mycobacterium tuberculosis
sitting drop vapor diffusion technique, PEG 3350, in complex with o-succinylbenzoyl-amino-CoA
Escherichia coli
Engineering
Amino acid exchange
Commentary
Organism
D185E
reduced kcat
Mycobacterium tuberculosis
D185G
no activity
Mycobacterium tuberculosis
D185N
reduced kcat
Mycobacterium tuberculosis
D192N
no activity
Mycobacterium tuberculosis
G156D
no activity
Escherichia coli
S190A
reduced kcat
Mycobacterium tuberculosis
Y287F
no activity
Mycobacterium tuberculosis
Y97F
no activity
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0031
-
2-succinylbenzoyl-CoA
D185N mutant protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.0048
-
2-succinylbenzoyl-CoA
D185E mutant protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.0224
-
2-succinylbenzoyl-CoA
wild type protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.0259
-
o-succinylbenzoyl-CoA
pH 7.0, 25C
Escherichia coli
0.0402
-
2-succinylbenzoyl-CoA
S190A mutant protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.106
-
2-(3-carboxypropyl)-benzoyl-CoA
wild type protein protein, pH 7.0, 25C
Mycobacterium tuberculosis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
6 * 30000, SDS-PAGE, crystallization
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Mycobacterium tuberculosis
P9WNP5
-
-
Purification (Commentary)
Commentary
Organism
-
Mycobacterium tuberculosis
His-tag affinity chromatography, tag removed
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-(3-carboxypropyl)-benzoyl-CoA
-
714273
Mycobacterium tuberculosis
?
-
-
-
?
2-succinylbenzoyl-CoA
-
714273
Mycobacterium tuberculosis
1,4-dihydroxy-2-naphthoyl-CoA + H2O
-
-
-
?
o-succinylbenzoyl-CoA
-
714273
Escherichia coli
1,4-dihydroxy-2-naphthoyl-CoA + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
hexamer
6 * 30000, SDS-PAGE, crystallization
Escherichia coli
hexamer
crystallization
Mycobacterium tuberculosis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00022
-
2-succinylbenzoyl-CoA
D185N mutant protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.00025
-
2-(3-carboxypropyl)-benzoyl-CoA
wild type protein protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.0017
-
2-succinylbenzoyl-CoA
S190A mutant protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.0023
-
2-succinylbenzoyl-CoA
D185E mutant protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.062
-
o-succinylbenzoyl-CoA
pH 7.0, 25C
Escherichia coli
0.462
-
2-succinylbenzoyl-CoA
wild type protein, pH 7.0, 25C
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3)
Mycobacterium tuberculosis
His-tagged version expressed in Escherichia coli BL21(DE3)
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion technique, PEG 6000, in complex with o-succinylbenzoyl-aminoCoA
Mycobacterium tuberculosis
sitting drop vapor diffusion technique, PEG 3350, in complex with o-succinylbenzoyl-amino-CoA
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D185E
reduced kcat
Mycobacterium tuberculosis
D185G
no activity
Mycobacterium tuberculosis
D185N
reduced kcat
Mycobacterium tuberculosis
D192N
no activity
Mycobacterium tuberculosis
G156D
no activity
Escherichia coli
S190A
reduced kcat
Mycobacterium tuberculosis
Y287F
no activity
Mycobacterium tuberculosis
Y97F
no activity
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0031
-
2-succinylbenzoyl-CoA
D185N mutant protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.0048
-
2-succinylbenzoyl-CoA
D185E mutant protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.0224
-
2-succinylbenzoyl-CoA
wild type protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.0259
-
o-succinylbenzoyl-CoA
pH 7.0, 25C
Escherichia coli
0.0402
-
2-succinylbenzoyl-CoA
S190A mutant protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.106
-
2-(3-carboxypropyl)-benzoyl-CoA
wild type protein protein, pH 7.0, 25C
Mycobacterium tuberculosis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
6 * 30000, SDS-PAGE, crystallization
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Mycobacterium tuberculosis
His-tag affinity chromatography, tag removed
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-(3-carboxypropyl)-benzoyl-CoA
-
714273
Mycobacterium tuberculosis
?
-
-
-
?
2-succinylbenzoyl-CoA
-
714273
Mycobacterium tuberculosis
1,4-dihydroxy-2-naphthoyl-CoA + H2O
-
-
-
?
o-succinylbenzoyl-CoA
-
714273
Escherichia coli
1,4-dihydroxy-2-naphthoyl-CoA + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
hexamer
6 * 30000, SDS-PAGE, crystallization
Escherichia coli
hexamer
crystallization
Mycobacterium tuberculosis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00022
-
2-succinylbenzoyl-CoA
D185N mutant protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.00025
-
2-(3-carboxypropyl)-benzoyl-CoA
wild type protein protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.0017
-
2-succinylbenzoyl-CoA
S190A mutant protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.0023
-
2-succinylbenzoyl-CoA
D185E mutant protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.062
-
o-succinylbenzoyl-CoA
pH 7.0, 25C
Escherichia coli
0.462
-
2-succinylbenzoyl-CoA
wild type protein, pH 7.0, 25C
Mycobacterium tuberculosis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0024
-
2-(3-carboxypropyl)-benzoyl-CoA
wild type protein protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.04
-
2-succinylbenzoyl-CoA
S190A mutant protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.07
-
2-succinylbenzoyl-CoA
D185N mutant protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.48
-
2-succinylbenzoyl-CoA
D185E mutant protein, pH 7.0, 25C
Mycobacterium tuberculosis
2.4
-
o-succinylbenzoyl-CoA
pH 7.0, 25C
Escherichia coli
21
-
2-succinylbenzoyl-CoA
wild type protein, pH 7.0, 25C
Mycobacterium tuberculosis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0024
-
2-(3-carboxypropyl)-benzoyl-CoA
wild type protein protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.04
-
2-succinylbenzoyl-CoA
S190A mutant protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.07
-
2-succinylbenzoyl-CoA
D185N mutant protein, pH 7.0, 25C
Mycobacterium tuberculosis
0.48
-
2-succinylbenzoyl-CoA
D185E mutant protein, pH 7.0, 25C
Mycobacterium tuberculosis
2.4
-
o-succinylbenzoyl-CoA
pH 7.0, 25C
Escherichia coli
21
-
2-succinylbenzoyl-CoA
wild type protein, pH 7.0, 25C
Mycobacterium tuberculosis
Other publictions for EC 4.1.3.36
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748290
Passi
RepTB a gene ontology based d ...
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24
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-
-
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12
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1
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1
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1
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12
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1
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-
-
-
1
-
-
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-
746618
Song
Ligand-dependent active-site ...
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70
2959-2969
2014
-
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1
1
-
-
-
-
-
-
-
2
-
4
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
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1
1
1
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-
-
2
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1
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-
-
2
1
-
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-
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-
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-
-
-
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-
728604
Sun
Structural basis of the induce ...
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PLoS ONE
8
e63095
2013
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2
2
4
-
4
4
-
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2
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4
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2
2
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4
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4
2
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2
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2
4
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4
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4
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2
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2
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-
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-
4
-
-
-
-
4
2
-
-
-
-
3
3
-
4
4
726985
Sun
Active site binding and cataly ...
Escherichia coli, Synechocystis sp.
Biochemistry
51
4580-4589
2012
-
-
2
2
-
-
-
-
-
2
-
2
-
2
-
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2
2
-
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-
2
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2
-
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-
2
-
2
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
6
6
-
-
-
714260
Chen
Stabilization of the second ox ...
Escherichia coli
Biochemistry
50
5893-5904
2011
-
-
1
-
8
-
4
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
1
-
-
8
-
-
4
4
-
-
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-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714273
Li
Mechanism of the intramolecula ...
Escherichia coli, Mycobacterium tuberculosis
Biochemistry
50
9532-9544
2011
-
-
2
2
8
-
-
6
-
-
1
-
-
4
-
-
2
-
-
-
-
-
3
2
-
-
-
6
-
-
-
-
-
-
-
-
-
2
-
2
8
-
-
-
-
6
-
-
1
-
-
-
-
2
-
-
-
-
3
2
-
-
-
6
-
-
-
-
-
-
-
-
6
6
726554
Li
CoA adducts of 4-oxo-4-phenylb ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
ACS Med. Chem. Lett.
2
818-823
2011
-
1
-
-
-
-
20
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
3
-
18
-
1
-
-
-
-
-
18
20
3
-
-
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-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
704888
Babujee
The proteome map of spinach le ...
Spinacia oleracea
J. Exp. Bot.
61
1441-1453
2010
-
-
-
-
-
-
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-
1
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1
-
6
-
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1
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1
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1
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1
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1
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1
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714417
Li
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Mycobacterium tuberculosis
Bioorg. Med. Chem. Lett.
20
6306-6309
2010
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1
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-
15
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-
3
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1
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-
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1
1
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-
-
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-
3
-
11
-
-
1
-
-
-
-
11
15
3
-
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1
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-
1
1
-
-
-
-
-
-
-
-
-
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-
-
-
-
690466
Lannergard
Identification of the genetic ...
Staphylococcus aureus
Antimicrob. Agents Chemother.
52
4017-4022
2008
-
1
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1
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4
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1
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1
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1
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1
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-
677366
Kanaujia
Cloning, expression, purificat ...
Geobacillus kaustophilus
Acta Crystallogr. Sect. F
63
103-105
2007
-
-
1
1
-
-
-
-
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1
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4
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1
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-
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1
1
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1
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1
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1
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1
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1
1
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-
677390
Ulaganathan
Structure of Staphylococcus au ...
Staphylococcus aureus
Acta Crystallogr. Sect. F
63
908-913
2007
-
-
1
1
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-
-
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1
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4
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1
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1
1
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1
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1
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1
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1
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1
1
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-
663500
Johnston
Structure of naphthoate syntha ...
Mycobacterium tuberculosis
Acta Crystallogr. Sect. D
61
1199-1206
2005
-
-
1
1
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-
-
-
-
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5
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1
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1
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1
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1
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-
93959
Kolkmann
4-(2'-Carboxyphenyl)-4-oxobuty ...
Escherichia coli, Galium mollugo, Mycolicibacterium phlei
Z. Naturforsch. C
42
1207-1214
1987
-
-
-
-
-
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-
-
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3
-
3
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3
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2
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6
-
3
-
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3
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3
-
2
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6
-
3
-
-
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-
-
-
-
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-
-
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-
93961
Shaw
-
Recombinent plasmids containin ...
Escherichia coli
FEMS Microbiol. Lett.
17
63-67
1983
-
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1
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1
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1
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1
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1
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93962
Heide
Enzymatic synthesis, character ...
Escherichia coli, Mycolicibacterium phlei
J. Biol. Chem.
257
7396-7400
1982
-
-
-
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2
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2
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2
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2
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2
2
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2
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2
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2
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2
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2
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2
2
-
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-
-
-
-
-
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-
-
-
-
93960
Heide
Enzymatic synthesis of the coe ...
Mycolicibacterium phlei
FEBS Lett.
128
201-204
1981
-
-
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93958
Meganathan
Menaquinone (vitamin K2) biosy ...
Mycolicibacterium phlei
J. Bacteriol.
140
92-98
1979
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