3.7.1.9: 2-hydroxymuconate-6-semialdehyde hydrolase

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For detailed information about 2-hydroxymuconate-6-semialdehyde hydrolase, go to the full flat file.

Word Map on EC 3.7.1.9

3.7.1.9

catechols

extradiol

sphingomonas

2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate

2-hydroxy-6-oxohepta-2,4-dienoate

lignin-related

4-hydroxy-2-oxovalerate

xylf

paucimobilis

5-carboxyvanillate

4-oxalocrotonate

m-cresol

isopropylbenzene

toluate

3-methylcatechol

molecular biology

Reaction

Synonyms

1H-3-Hydroxy-4-oxoquinaldine 2,4-dioxygenase, 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate, 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase, 2-hydroxy-6-oxohepta-2,4-dienoate hydrolase, 2-hydroxymuconate semialdehyde hydrolase, 2-hydroxymuconate-semialdehyde hydrolase, 2-hydroxymuconic semialdehyde hydrolase, 6-isopropyl-HODA hydrolase, CumD, HcdC, HMSH, HOD, HOD hydrolase, hydrolase, 2-hydroxymuconate semialdehyde, hydroxymuconic semialdehyde hydrolase, meta-cleavage compound hydrolase, meta-cleavage product hydrolase, MfhA, TodF, XylF

ECTree

3 Hydrolases

3.7 Acting on carbon-carbon bonds

3.7.1 In ketonic substances

3.7.1.9 2-hydroxymuconate-6-semialdehyde hydrolase

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Results	in table
2	Activating Compound
391	AA Sequence
4	Application
1	CAS Registry Number
8	Cloned(Commentary)
2	Crystallization (Commentary)
6	General Information
19	Inhibitors
3	kcat/KM [mM/s]
74	KM Value [mM]
1	Metals/Ions
14	Molecular Weight [Da]
17	Natural Substrates/ Products (Substrates)
70	Organism
10	Pathway
11	PDB ID
6	pH Optimum
1	pH Range
3	pH Stability
1	pI Value
41	Protein Variants
8	Purification (Commentary)
1	Reaction
1	Reaction Type
33	Reference
10	Specific Activity [micromol/min/mg]
1	Storage Stability
96	Substrates and Products (Substrate)
20	Subunits
51	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
2	Temperature Range [°C]
3	Temperature Stability [°C]
48	Turnover Number [1/s]

Purification

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Purification on EC 3.7.1.9 - 2-hydroxymuconate-6-semialdehyde hydrolase

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2 entries

ATCC 17707, strain 345, isozyme HODHII: TOL-like plasmid encoded enzyme, strain RA1012, isozyme HODHI: chromosomally-encoded enzyme

Cupriavidus necator

247051

by affinity chromatography

Flavobacterium sp.

671782

His-tagged HOD purified by Ni2+/NTA affinity chromatography

Arthrobacter sp.

674348

recombinant enzyme from Escherichia coli strain JM109 by anion exchange chromatography and gel filtration

Pseudomonas sp.

G3KFX4

733435

to homogeneity, gel filtration

Pseudomonas fluorescens

674958