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Literature summary for 3.7.1.9 extracted from

  • Bayly, R.C.; Diberardino, D.; Hughes, E.J.L.; Skurray, R.A.
    The purification and properties of two isofunctional 2-hydroxy-6-oxohepta-2,4-dienoate hydrolase from Alcaligenes eutrophus strain 345 (1987), J. Gen. Microbiol., 133, 815-822.
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Search for more literature for 3.7.1.9

Activating Compound

Activating Compound Comment Organism Structure
Mn2+ isoenzyme HODHI is inhibited, isoenzyme HODHII is activated Cupriavidus necator

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Cupriavidus necator
Mn2+ isoenzyme HODHI is inhibited, isoenzyme HODHII is activated Cupriavidus necator
PCMB
-
 Cupriavidus necator
Zn2+
-
 Cupriavidus necator

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 2-hydroxy-6-oxohepta-2,4-dienoate isoenzyme HODHII Cupriavidus necator
0.058
-
 2-hydroxy-6-oxohepta-2,4-dienoate isoenzyme HODHI Cupriavidus necator
0.36
-
 2-hydroxymuconic semialdehyde isoenzyme HODHI Cupriavidus necator
62
-
 2-hydroxymuconic semialdehyde isoenzyme HODHII Cupriavidus necator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34400
-
 3 * 34400, isoenzyme HODHI, SDS-PAGE of 2-mercaptoethanol reduced enzyme Cupriavidus necator
36700
-
 3 * 36700, isoenzyme HODHII, Alcaligenes eutrophus, SDS-PAGE of 2-mercaptoethanol reduced enzyme Cupriavidus necator
104000
-
 sedimentation equilibrium analysis Cupriavidus necator
116000
-
 isozyme HODHII, sedimentation equilibrium analysis Cupriavidus necator

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
 ATCC 17707
-

Purification (Commentary)

Purification (Comment) Organism
ATCC 17707, strain 345, isozyme HODHII: TOL-like plasmid encoded enzyme, strain RA1012, isozyme HODHI: chromosomally-encoded enzyme Cupriavidus necator

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.1
-
 isoenzyme HODHII Cupriavidus necator
17.4
-
 isoenzyme HODHI Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-5-methyl-6-oxohepta-2,4-dienoate + H2O no activity with isoenzyme HODHI, 41% of the activity with 2-hydroxy-6-oxohepta-2,4-dienoate, isoenzyme HODHII Cupriavidus necator ?
-
 ?
2-hydroxy-5-methyl-muconic semialdehyde + H2O 3% of the activity with 2-hydroxy-6-oxohepta-2,4-dienoate, isoenzyme HODHI. 5% of the activity with 2-hydroxy-6-oxohepta-2,4-dienoate, isoenzyme HODHI Cupriavidus necator ?
-
 ?
2-hydroxy-6-oxohepta-2,4-dienoate + H2O
-
 Cupriavidus necator ?
-
 ?
2-hydroxy-7-methyl-6-oxoocta-2,4-dienoate + H2O 149% of the activity with 2-hydroxy-6-oxohepta-2,4-dienoate, isoenzyme HODHI, no activity with isoenzyme HODHII Cupriavidus necator ?
-
 ?
2-hydroxymuconate semialdehyde + H2O 31% of the activity with 2-hydroxy-6-oxohepta-2,4-dienoate, isoenzyme HODHI. 12% of the activity with 2-hydroxy-6-oxohepta-2,4-dienoate, isoenzyme HODHII Cupriavidus necator formate + 2-oxopent-4-enoate
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 34400, isoenzyme HODHI, SDS-PAGE of 2-mercaptoethanol reduced enzyme Cupriavidus necator
trimer 3 * 36700, isoenzyme HODHII, Alcaligenes eutrophus, SDS-PAGE of 2-mercaptoethanol reduced enzyme Cupriavidus necator

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 isoenzyme HODHII is inactivated after 2 min, isoenzyme HODHI is inactivated after 4 min Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
-
 Cupriavidus necator

pH Range

pH Minimum pH Maximum Comment Organism
5 10 pH 7.6: 75-80% of the activity at pH 8.6, pH 9.0: marked decrease in activity above pH 9.0, similar pH profile of both isoenzymes Cupriavidus necator