Cloned (Comment) | Organism |
---|---|
into pUC119 and overexpression in Escherichia coli | Pseudomonas fluorescens |
Protein Variants | Comment | Organism |
---|---|---|
D224A | aggregates into inclusion bodies and thus can not be purified | Pseudomonas fluorescens |
H252A | aggregates into inclusion bodies and thus can not be purified | Pseudomonas fluorescens |
S103A | has 600000fold lower activity than that of the wild-type enzyme, can be found in the soluble fraction and thus can be purified | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PMSF | 0.01 mM strongly inhibits, CumD activity being undetectable | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00074 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
Pseudomonas fluorescens | |
0.0073 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | 150 mM phosphate, 25°C, pH 7.5 | Pseudomonas fluorescens | |
0.009 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | - |
Pseudomonas fluorescens | |
0.0095 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | - |
Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31489 | - |
2 * 32000, SDS-PAGE, 2 * 31489, amino acid sequence | Pseudomonas fluorescens |
32000 | - |
2 * 32000, SDS-PAGE, 2 * 31489, amino acid sequence | Pseudomonas fluorescens |
58000 | - |
gel filtration | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
IPO1 | - |
Pseudomonas fluorescens IPO1 | - |
IPO1 | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, gel filtration | Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.1 | - |
S103A mutant | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | slight hydrolysis | Pseudomonas fluorescens | ? | - |
? | |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | slight hydrolysis | Pseudomonas fluorescens IPO1 | ? | - |
? | |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O | - |
Pseudomonas fluorescens | ? | - |
? | |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O | - |
Pseudomonas fluorescens IPO1 | ? | - |
? | |
2-hydroxy-6-oxohepta-2,4-dienoate + H2O | - |
Pseudomonas fluorescens | ? | - |
? | |
2-hydroxy-6-oxohepta-2,4-dienoate + H2O | - |
Pseudomonas fluorescens IPO1 | ? | - |
? | |
2-hydroxy-6-oxoocta-2,4-dienoate + H2O | - |
Pseudomonas fluorescens | ? | - |
? | |
2-hydroxy-6-oxoocta-2,4-dienoate + H2O | - |
Pseudomonas fluorescens IPO1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, SDS-PAGE, 2 * 31489, amino acid sequence | Pseudomonas fluorescens |
Synonyms | Comment | Organism |
---|---|---|
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase | - |
Pseudomonas fluorescens |
CumD | - |
Pseudomonas fluorescens |
meta-cleavage compound hydrolase | - |
Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
47.5 | - |
- |
Pseudomonas fluorescens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 60 | full enzyme activity up to 20°C for 30 min, and ca. 80% between 30 and 50°C, almost completely inactivated above 60°C | Pseudomonas fluorescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
stable for 3 days | Pseudomonas fluorescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
Pseudomonas fluorescens | |
18 | - |
2-hydroxy-6-oxohepta-2,4-dienoate | - |
Pseudomonas fluorescens | |
21 | - |
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate | 150 mM phosphate, 25°C, pH 7.5 | Pseudomonas fluorescens | |
41 | - |
2-hydroxy-6-oxoocta-2,4-dienoate | - |
Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas fluorescens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Pseudomonas fluorescens |