3.5.5.8: thiocyanate hydrolase

This is an abbreviated version!
For detailed information about thiocyanate hydrolase, go to the full flat file.

Word Map on EC 3.5.5.8

3.5.5.8

thiobacillus

thioparus

sulfide

nitrile

hydratase

ammonia

nhases

metalloenzyme

thiosulfate

cyanase

coiii

cobalt-containing

non-heme

coke

nh3

Reaction

+ 2 H2O =

Synonyms

SCNase

ECTree

3 Hydrolases

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.5 In nitriles

3.5.5.8 thiocyanate hydrolase

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Results	in table
2	Activating Compound
220	AA Sequence
1	CAS Registry Number
6	Cloned(Commentary)
3	Crystallization (Commentary)
11	Inhibitors
1	Ki Value [mM]
4	KM Value [mM]
7	Metals/Ions
17	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
28	Organism
2	Oxidation Stability
1	Pathway
84	PDB ID
3	pH Optimum
2	Posttranslational Modification
4	Protein Variants
6	Purification (Commentary)
1	Reaction
1	Reaction Type
19	Reference
2	Source Tissue
4	Specific Activity [micromol/min/mg]
1	Storage Stability
22	Substrates and Products (Substrate)
13	Subunits
11	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
2	Temperature Range [°C]
3	Temperature Stability [°C]
3	Turnover Number [1/s]

Purification

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Purification on EC 3.5.5.8 - thiocyanate hydrolase

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2 entries

ammonium sulfate precipitation, DEAE-Sephacyl, hydroxylapatite

Thiobacillus thioparus

209767

DEAE-Sepharose column chromatography, Mono Q GI column chromatography, and Superdex 200 gel filtration

Thiohalophilus thiocyanatoxydans

678457

hydroxyapatite column chromatography (native enzyme) and glutathione Sepharose column chromatography (recombinant enzyme). The activity of the enzyme purified at room temperature is 1.4times that of the enzyme purified at 4°C

Alphaproteobacteria

734642

native enzyme from strain THI115, and recombinant enzyme from Echerichia coli strain BL21(DE3) by three steps of butyl-resin chromatography, hydroxyapatite and ion exchange chromatography, or by 4 steps of butyl-resin chromatography and gel filtration

Thiobacillus thioparus

668637